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Gupta A.K.,University Institute of Pharmacy | Kumar P.,Moradabad Educational Trust Group of Institutions
International Journal of Diabetes in Developing Countries | Year: 2010

Background: Onosma hispidum (Ratanjot) belongs to the family Boraginaceae. The genus Onosma has about 150 known species in Asia. It has been found to be effective as an antioxidant, anti-inflammatory, and antimicrobial agent and promotes healing in burns, foot ulcers, and wounds. Aim: To study the effect of O. hispidum on blood glucose level in glucose-loaded, normal, and hyperglycemic rats. Settings: Department of Pharmacy, Bundelkhand University, Jhansi, Uttar Pradesh, India. Design: An experimental study was designed. Materials and Methods: The methanolic root extracts of O. hispidum are compared with glibenclamide for their influence on fasting blood glucose in glucose-loaded, normoglycemic, and alloxan-induced (120 mg/kg i.p) hyperglycemic rats. Statistical Analysis: The data were analysed by one way analysis of variance followed by Dunnett′s test (P < 0.01). Results: In glucose-loaded, normal and hyperglycemic rats, the methanolic root extract of O. hispidum at the dose of 100 mg/kg p.o. reduced blood glucose significantly as compared to control, and it was almost as effective as glibenclamide.Conclusion: The methanolic root extract of O. hispidum has hypoglycemic action. As diabetes is associated with hyperglycemia and several other pathological changes such as infections, inflammation, foot ulcers and impairment of wound healing, these additional effects besides hypoglycemic effect of O. hispidum may be proved as a breakthrough in the treatment of diabetes. Biochemical and receptor oriented molecular studies are required to find out the exact mechanism of hypoglycemic action of O. hispidum. Source

Gupta P.C.,University Institute of Pharmacy | Gupta P.C.,Bhagwant University | Rao C.V.,National Botanical Research Institute
Indian Journal of Natural Products and Resources | Year: 2012

Cleome viscosa Linn. (Family: Capparaceae) is an annual, sticky herb and commonly known as wild or dog mustard. It has various ethnomedicinal values as various traditional communities find diverse medicinal properties. This work aimed to study the macro- and microscopically, physicochemical, photochemical and fluorescence analysis of different parts of the C. viscosa. Macro- and microscopically studies showed the presence of palmate compound leaf with 3-5 leaflets, obviate to lanceolate, glandular and simple covering trachoma's and animistic arrangement of stomata. Sclerenchmatous caped vascular bundle in petiole, arc shaped per cyclic fibers in stem and distinct uni to biseriate medullary rays of root are some of the diagnostic features noted from anatomical study of the plant. Powder microscopy revealed the presence of fibres, spiral and pitted vessels, trichomes and calcium oxalate crystal. Total ash of leaf was about two times higher than root and about four times higher than seed. The fluorescent analysis under day light and UV light by treatment with different chemical reagents showed different colour. Phytochemical evaluation revealed the presence of triterpenoids, saponins, tannins, flavonoids and steroids. Source

Gupta P.C.,University Institute of Pharmacy | Rao C.V.,National Botanical Research Institute
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To study morpho-anatomical characters and physicochemical analysis of . Fumaria indica (. F. indica) (Hausskn.) Pugsley, (Fumariaceae), an important medicinal plant used extensively for treating a variety of ailments in various system of indigenous medicine. Methods: Evaluation of the different parts of the plant was carried out to determine the morpho-anatomical, physicochemical, phytochemical and HPTLC fingerprinting profile of . F. indica and other WHO recommended methods were performed for standardization. Results: Morpho-anatomical studies showed compound and pinnatifid leaf, 4 to 6 cm in length, linear and oblong in shape and anomocytic arrangement of stomata, thin walled parenchymatous cells, scattered, sclerenchymatous, capped vascular bundles and radiating medullary rays. Physicochemical studies showed foreign matter 0.2%, loss on drying 6.8%, total ash 16.77%, alcohol and water soluble extractives 8.92% and 20.26%, respectively, sugar 17.75%, starch 22.97% and tannins 2.37%. Phytochemical evaluation revealed the presence of carbohydrate, alkaloids, flavonoids, saponins, tannins and sterol. Thin layer chromatography was carried out with different solvents and the best solvent system was chloroform and methanol in 80:20 ratio and revealed 12 spots with different Rf value under UV light 366λ. Conclusion: The results of the study can serve as a valuable source of information and provide suitable standards for identification of this plant material for future investigations and applications. © 2012 Asian Pacific Tropical Biomedical Magazine. Source

Sachan A.K.,Institute of Pharmacy | Sachan N.K.,University Institute of Pharmacy | Kumar S.,Institute of Pharmacy | Sachan A.,Institute of Pharmacy | Gangwar S.S.,Gsvm Government Medical College
European Journal of Scientific Research | Year: 2010

Conventional means for delivery of essential oils are elixirs or the soft gels. In present study, the chewable tablets are proposed as means for their utility in delivery of volatile therapeutic ingredients like essential oils as a better alternative of conventional options. This study envisage the pharmaceutical characterization and evaluation of caraway oil and peppermint oil through marker compounds using TLC, HPLC, HPTLC and gas chromatography. The two oils are used internally for symptomatic treatment of irritable bowel syndrome, and digestive disorders such as flatulence, gastritis antispasmodic, carminative, analgesic and for cure of dyspepsia. The oils were loaded to granules previously standardized through GC and HPTLC for their identity and quantification of marker compound, and their compatibility testing with excipients was performed using TLC and HPLC. Accelerated stability testing, for availability of marker compound in formulated tablets, conducted by storing the tablets under different stress conditions. © EuroJournals Publishing, Inc. 2010. Source

Gupta P.C.,University Institute of Pharmacy | Sharma N.,University Institute of Pharmacy | Rao C.V.,National Botanical Research Institute
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To study detailed pharmacognostic profile of leaves and stem of Careya arborea (C. arborea) Roxb. (Lecthyidaceae), an important medicinal plant in the Indian system of medicine. Methods: Leaf and stem samples of C. arborea were studied by macroscopical, microscopical, physicochemical, phytochemical, fluorescence analysis of powder of the plant and other methodsfor standardization recommended by WHO. Results: Macroscopically, the leaves are simple, broadly obovate in shape, acuminate apex with crenate, dentate margin, petioles (0.1-1.8 cm) long. Microscopically, the leaf showed the presence of median large size vascular bundle covered with fibrous bundle sheath, arrangement of xylem in cup shape and presence of cortical vascular bundle, patches of sclerenchyma, phloem fibers in groups and brown pigment containing cells in stem aresome of the diagnostic features noted from anatomical study. Powder microscopy of leaf revealed thepresence of parenchyma cells, xylem with pitted vessels and epidermis with anisocytic stomata. Theinvestigations also included leaf surface data; quantitative leaf microscopy and fluorescence analysis. Physiochemical parameters such as loss on drying, swelling index, extractive values and ash valueswere also determined and results showed that total ash of the stem bark was about two times higherthan leaf and water soluble extractive value of leaf and stem bark was two times higher than alcoholsoluble extractive value. Preliminary phytochemical screening showed the presence of triterpenoids, saponins, tannins and flavonoids. Conclusions: The results of the study can serve as a valuablesource of information and provide suitable standards for identification of this plant material in futureinvestigations and applications. Source

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