Blom E.,Herlev University Hospital Herlev |
Koziorowski J.,Herlev University Hospital Herlev
Journal of Radioanalytical and Nuclear Chemistry | Year: 2014
In cancer cells hypoxia can cause resistance to both radio- and chemo-therapy. Being able to quantify, the degree of hypoxia in the cells is a useful tool in therapy planning. The positron emitting 1-[18F]fluoro- 3-(2-nitro-1H-imidazol-1-yl)propan-2-ol ([18F]FMISO) is the most extensively used tracer for imaging hypoxia. Automated synthesis of [ 18F]FMISO was set up on IBA Synthera®. The precursor 1-(2′-nitro-1′-imidazolyl)-2-O-tetrahydropyranyl-3-O-p- toluenesulfonyl propanediol (NITTP) was heated at 100 C for 10 min with [K/K 2.2.2.]+[18F]- and thereafter hydrolyzed with 0.1 M hydrochloric acid at 90 C for 2 min. Purification was performed on solid-phase extraction (SPE) cartridges. [18F]FMISO was obtained in 50 ± 3 % (n = 6) radiochemical yield (decay-corrected) in 35 min synthesis time with radiochemical purity of ≥98 %. The use of disposable Integrated Fluid Processors (IFP™:s) and cartridge purification simplifies the handling and shortens the synthesis time. This is a no frills setup based on all commercially available materials and the synthesis is performed with minor changes from the FDG time-list. © 2013 Akadémiai Kiadó, Budapest, Hungary.