Time filter

Source Type

Duarte M.S.,University Federal Of Vicosamg | Paulino P.V.R.,University Federal Of Vicosamg | Nascimento C.S.,University Federal Of Vicosamg | Botelho M.E.,University Federal Of Vicosamg | And 7 more authors.
Journal of Animal Science

Twenty-four pregnant Nellore cows were randomly assigned into 2 feeding level groups (control [CTL]; fed 1.0 times the maintenance requirement; n = 12; and overnourished [ON]; fed at 1.5 times the maintenance requirement; n = 12) to evaluate effects of maternal overnutrition on fetal skeletal muscle development. Cows were slaughtered at 135, 190, and 240 d of gestation and samples of fetal LM were collected for analysis of mRNA expression analysis and for histological evaluation of collagen content and number of muscle cells. There was no interaction between gestational period and maternal nutrition for the variables evaluated (P > 0.05). The mRNA expression ofCadherin-associated protein, β 1 (β-catenin) tended to be greater in fetuses from ON cows (P = 0.08), while myogenic differentiation 1 (MyoD;P = 0.56), myogenin (MyoG; P = 0.70), and the number of muscle cells (P= 0.90) were not affected by maternal overnutrition. Gestational period did not affect the mRNA expression of β-catenin (P = 0.60) and MyoG (P= 0.21). The mRNA expression of MyoD tended to increase with days of gestation (P = 0.06). The mRNA expression of zinc finger protein 423(Zfp423; P < 0.0001), C/EBPα (P = 0.01), and PPARγ (P < 0.0001) were enhanced in ON fetuses. No effects of days of gestation were observed for mRNA expression of Zfp423 (P = 0.75) and C/EBPα (P = 0.48). The mRNA expression of PPARγ in fetuses at 190 d of gestation tended to be greater than those at 135 and 240 d of gestation (P = 0.06). The mRNA expression of transforming growth factor β (TGF-β; P < 0.0001), collagen type III, α I (COL3A1; P < 0.0001), and collagen content (P = 0.01) were increased in ON fetuses. Gestational period did not affect the mRNA expression of collagen type I, α I (COL1A1; P = 0.65). The mRNA expression of COL3A1 (P = 0.09) in fetuses at 190 d of gestation tended to be greater than fetuses at 135 and 240 d of gestation. The mRNA expression of TGF-β in fetuses at 190 d of gestation was greater than in fetuses at 135 d of gestation (P = 0.03), and the values observed in fetuses at 240 d of gestation did not differ from the other gestational time points. The least value of collagen content (P = 0.01) was observed in fetuses at 135 d of gestation, and no differences were observed among the other gestational time points. These data shows that maternal overnutrition enhances fibrogenesis and likely adipogenesis without compromising myogenesis in fetal skeletal muscle of cattle. © 2014 American Society of Animal Science. All rights reserved. Source

Righi T.,University Federal Of Vicosamg | De Carvalho C.A.,University Federal Of Vicosamg | Ribeiro L.M.,University Federal Of Vicosamg | da Cunha D.N.Q.,University Federal Of Vicosamg | And 4 more authors.
Revista Brasileira de Medicina do Esporte

Introduction: Biomarkers have been used to monitor the use of alcohol and currently the most sensitive and specific are the liver enzymes, for example, gamma-glutamyl transferase (GGT), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). Objective: To determine through animal experiments the changes caused by alcohol and the physical activity in liver and pancreatic enzymes. Methods: Twenty-four Wistar rats were randomly assigned into experimental groups, housed in cages with controlled environment, divided according to the treatment received. In the initial treatment, alcohol was administered to sedentary alcohol (SA) and exercised alcohol (EA) groups and at the end of the fourth week the program of physical training on a treadmill began with the AE and control exercised (CE) groups. Blood collection was performed by cardiac puncture at the end of each experiment. For the statistical analysis we used analysis of variance (ANOVA) followed by Tukey test and Kruskal-Wallis test. Results: The AS group had significantly higher values of ALT and ALP when compared to CE and EA groups, respectively. No significant differences were observed between the four groups for the parameters AST, GGT and amylase. Conclusion: The association between alcohol consumption and physical inactivity increased the release of the enzymes ALT and ALP in Wistar rats; the practice of aerobic exercise after alcohol withdrawal prevented the increased release of ALP in the plasma of these animals. © 2016, Redprint Editora Ltda. All rights reserved. Source

Discover hidden collaborations