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Heimfarth L.,Federal University of Rio Grande do Sul | da Silva Ferreira F.,Federal University of Rio Grande do Sul | Pierozan P.,Federal University of Rio Grande do Sul | Loureiro S.O.,Federal University of Rio Grande do Sul | And 4 more authors.
Biochimica et Biophysica Acta - General Subjects | Year: 2016

Background Diphenylditelluride (PhTe)2 is a potent neurotoxin disrupting the homeostasis of the cytoskeleton. Methods Cultured astrocytes and neurons were incubated with (PhTe)2, receptor antagonists and enzyme inhibitors followed by measurement of the incorporation of [32P]orthophosphate into intermediate filaments (IFs). Results (PhTe)2 caused hyperphosphorylation of glial fibrillary acidic protein (GFAP), vimentin and neurofilament subunits (NFL, NFM and NFH) from primary astrocytes and neurons, respectively. These mechanisms were mediated by N-methyl-D-aspartate (NMDA) receptors, L-type voltage-dependent calcium channels (L-VDCCs) as well as metabotropic glutamate receptors upstream of phospholipase C (PLC). Upregulated Ca2 + influx activated protein kinase A (PKA) and protein kinase C (PKC) in astrocytes causing hyperphosphorylation of GFAP and vimentin. Hyperphosphorylated (IF) together with RhoA-activated stress fiber formation, disrupted the cytoskeleton leading to altered cell morphology. In neurons, the high intracellular Ca2 + levels activated the MAPKs, Erk and p38MAPK, beyond PKA and PKC, provoking hyperphosphorylation of NFM, NFH and NFL. Conclusions Our findings support that intracellular Ca2 + is one of the crucial signals that modulate the action of (PhTe)2 in isolated cortical astrocytes and neurons modulating the response of the cytoskeleton against the insult. General significance Cytoskeletal misregulation is associated with neurodegeneration. This compound could be a valuable tool to induce molecular changes similar to those found in different pathologies of the brain. © 2016 Elsevier B.V.

Pinto J.A.V.,University Federal Fronteira Sul | Thewes F.R.,Federal University of Santa Maria | Schorr M.R.W.,Federal University of Santa Maria | Ceconi D.L.,Federal University of Santa Maria | And 3 more authors.
Bioscience Journal | Year: 2015

In order to obtain more consumers approval, fruit require high quality during commercialization, making the improvement of new storage technologies necessary. Thus, the aim of this study was to evaluate the best mass loss level on ‘Murcott’ tangor quality maintenance after cold storage during 10 weeks. The treatments evaluated were: [1] 0% of mass loss (100 % of relative humidity); [2] 3% of mass loss; [3] 6% of mass loss and [4] 9% of mass loss, with 5 replicates of 18 fruits each treatment. The storage temperature was maintained at 4.0°C (±0.2°C). The experiment was conducted in a completely randomized design. After 10 weeks of storage plus seven days of shelf life at 20°C it was verified that with the mass loss increase, the decay incidence and the succulence are suppressed, mass loss also increased the soluble solids and titratable acidity. The best mass loss level for ‘Murcott’ tangor storage stay between 3 up to 6% because it reduces the decay incidence and turgor loss and maintain chemical qualities, such as ascorbic acid, soluble solids and titratable acidity. © 2015, Universidade Federal de Uberlandia. All rights reserved.

da Silva D.S.,University Federal Of Pelotasrs | da Silva C.E.H.,University Federal Of Pelotasrs | Soares M.S.P.,University Federal Of Pelotasrs | Azambuja J.H.,University Federal Of Pelotasrs | And 6 more authors.
European Journal of Medicinal Chemistry | Year: 2016

The present study assessed the biological potential of fourteen 1,3-thiazolidin-4-ones evaluating the antiglioma effect through decreasing of cell viability of glioblastoma multiform cells. The new compounds were efficient synthesized through multicomponent or multicomponent one-pot procedures in moderate to good yields (22–86%) from two arenealdehydes (4-(methylthio)benzaldehyde and 4-(methylsulfonyl)benzaldehyde), seven amines (aromatic and aliphatic) and mercaptoacetic acid. The compounds were identified and characterized by GC/MS and NMR, five of them by HRMS. Six thiazolidinones showed significant effect of decreasing cell viability compared to standard drug TMZ at 100 μM in 72 h in C6 cell line by MTT assay. The compounds 5b, 5e, 5g and 6e showed the best results in the screening at 100 μM and were analyzed at different concentrations (5, 25, 50, 100 and 250 μM). Compounds 5b and 5e showed statistical difference at 5 μM, 6e at 25 μM and 5g at 50 μM in 72 h of treatment. The cytotoxicity study in primary astrocytes cells was evaluated and none of fourteen compounds showed toxicity at 100 μM, eight of them were not cytotoxic at 250 μM, both in 72 h. In addition, the propidium iodide assay demonstrated that the compounds might induce cell death by necrosis. In conclusion, this work reports at least four compounds (5b, 5e, 5g and 6e) with potential anti-tumor effect against glioblastoma multiform cell presenting activity at low concentrations and safe profile of cytotoxicity. © 2016 Elsevier Masson SAS

Baldissera M.D.,Federal University of Santa Maria | Souza C.F.,Federal University of Santa Maria | Doleski P.H.,Federal University of Santa Maria | Junior G.B.,Federal University of Santa Maria | And 2 more authors.
Microbial Pathogenesis | Year: 2016

The aim of this study was to evaluate the cholinergic (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)) and purinergic (adenosine deaminase (ADA)) systems in head kidney, spleen, total blood and serum samples in experimentally infected fish with A. hydrophila, and the involvement of these systems during the inflammatory process. Silver catfish (Rhamdia quelen) juveniles were divided into two groups with seven fish each: uninfected (negative control) and infected (positive control). On day 2 post-infection, animals were euthanized and the head kidney, spleen, total blood and serum were collected. AChE and ADA activities in head kidney and spleen decreased in infected animals compared to uninfected animals, as well as AChE in total blood and seric ADA activities. BChE activity was not expressed in the evaluated tissues. Therefore, our results lead to the hypothesis that cholinergic and purinergic systems play an important role on the immune response against A. hydrophila with an anti-inflammatory effect. In summary, AChE and ADA activities reduced probably in order to protect against tissue inflammatory damage caused by infection. © 2016 Elsevier Ltd

Dalla Nora M.,University Federal Of Santa Mariars | Horner R.,University Federal Of Santa Mariars | de Carli D.M.,Servico Of Gastroenterologia Do Hospital Universitario | da Rocha M.P.,Servico Of Patologia Do Hospital Universitario | And 3 more authors.
Arquivos de Gastroenterologia | Year: 2016

Background - The diagnosis of H. pylori infection can be performed by non-invasive and invasive methods. The identification through a fecal antigen test is a non-invasive, simple, and relatively inexpensive test. Objective - To determine the diagnostic performance of fecal antigen test in the identification of H. pylori infection. Methods - H. pylori antigens were identified in the stools of dyspeptic patients undergoing upper gastrointestinal endoscopy. For the identification of H. pylori antigen, we use ImmunoCard STAT! HpSA with immunochromatography technique. Histopathology plus urease test were the gold standard. Results - We studied 163 patients, 51% male, mean age of 56.7± 8.5years. H. pylori infection was present in 49%. Fecal test presented: sensitivity 67.5% (CI95% 60.6-72.9); specificity 85.5% (CI95% 78.9-90.7); positive predictive value 81.8% (CI95% 73.4-88.4) and negative predictive value 73,2% (CI95% 67.5-77.6); Positive likelihood ratio was 4.7 (CI95% 2.9-7.9) and Negative Likelihood Ratio 0.4 (CI95% 0.3-0.5). The prevalence odds ratio for a positive test was 12.3 (CI95% 5.7-26.3). The index kappa between FAT and histology/urease test was 0.53 (CI95% 0.39-0.64). Conclusion - Immunochromatographic FAT is less expensive than the other methods and readily accepted by the patients but its diagnostic performance does not recommend its use in the primary diagnosis, when the patient may have an active infection. © 2016, Arquivos de Gastroenterologia. All rights reserved.

de Avila L.A.,Federal University of Pelotas | Martini L.F.D.,Federal University of Pelotas | Mezzomo R.F.,University Federal Of Santa Mariars | Refatti J.P.,Federal University of Pelotas | And 6 more authors.
Agronomy Journal | Year: 2015

In the Brazilian state of Rio Grande do Sul, rice (Oryza sativa L.) is predominantly grown using continuous food irrigation, which requires large quantities of fresh water. Due to increasing scarcity and demand for water, modern agricultural systems need to produce more food with less water. The objective of this study was to evaluate the effect of different irrigation management systems on water use effciency, quantity of water loss due to runof, and rice grain yield. The experiments were conducted from 2007 through 2010 at an experimental station of the Plant Protection Department of the Universidade Federal de Santa Maria, Rio Grande do Sul state, Brazil, on an Albaqualf sandy-loam soil. Treatments consisted of two irrigation management systems: continuous fooding (CF) at approximately a 10-cm food depth and intermittent irrigation (II) where the food was allowed to fully subside before being re-established to a 10-cm depth. Intermittent irrigation increased storage of rainwater by reducing runof water by 56% compared with CF. In addition, II reduced irrigation water use by 22 to 76% resulting in a 15 to 346% increase in water use effciency (WUE). Rice yield was not affected by II compared with CF. Intermittent irrigation provides greater water conservation, does not reduce rice yield compared with CF, and improves the WUE of rice. © 2015 by the American Society of Agronomy, 5585 Guilford Road, Madison, WI 53711. All rights reserved.

Sartor L.R.,Federal Technological University of Paraná | Lopes L.,Federal Technological University of Paraná | Martin T.N.,University Federal Of Santa Mariars | Ortiz S.,Federal Technological University of Paraná
Bioscience Journal | Year: 2015

Agrosilvipastoris systems are expanding the spaces occupied and gaining new adherents in order expansions productive in the same areas. But some issues, such as allelopathic effects of some cultures, in which case the pine, are still poorly known. This research aimed to evaluate the allelopathic effect of aqueous extract from the pine needles on the germination and development of lettuce, corn and beggarticks seedlings. The experiment was carried in a completely randomized design with treatments arranged in a factorial (2x5), where: conditions of the needles (green and dry) x extracts concentrations (0, 25, 50, 75 and 100%) in four replications, under temperature, humidity and light controlled. Evaluations were carried out time intervals of 24 hours, from 0 to 144 hours. It was evaluated the germination, average speed of germination, the length of the primary roots of lettuce and the length from the seedlings roots of lettuce, beggarticks and corn. Extracts the base of green needles affected the variables, where the effect increased with increase in concentration, the effect being more marked in beggarticks. In the statement the basis of dry pine needles, there were minimal results when compared to control. © 2015, Universidade Federal de Uberlandia. All rights reserved.

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