University Federal Of Santa Mariars

Brazil

University Federal Of Santa Mariars

Brazil
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Reginato C.F.,Federal University of Santa Maria | Bandeira L.A.,Federal University of Santa Maria | Zanette R.A.,Grande Rio University | Santurio J.M.,University Federal Of Santa Mariars | And 2 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2017

Introduction: This study evaluated the susceptibilities of oral candidiasis-derived Candida albicans, fluconazole-resistant (FR) Candida dubliniensis, and fluconazole-susceptible (FS) C. dubliniensis to synthetic antiseptics [chlorhexidine gluconate (CHX), cetylpyridinium chloride (CPC), and triclosan (TRC)] and natural compounds (carvacrol, eugenol and thymol). Methods: Susceptibility tests were performed based on the M27-A3 reference method. The fluconazole-resistant C. dubliniensis strains were obtained after prolonged in vitro exposure to increasing fluconazole concentrations. The geometric mean values for minimum inhibitory concentrations and minimum fungicidal concentrations were compared among the groups. Results: FS C. dubliniensis was more sensitive to CPC and TRC than FR C. dubliniensis and C. albicans. However, eugenol and thymol were more active against FR C. dubliniensis. The fungicidal activities of CHX and TRC were similar for the three groups, and FR C. dubliniensis and C. albicans had similar sensitivities to CPC. Conclusions: The resistance of C. dubliniensis to fluconazole affects its sensitivity to the synthetic antiseptics and natural compounds that were tested. © 2017, Sociedade Brasileira de Medicina Tropical. All rights reserved.


Favero J.F.,Santa Catarina State University | de Araujo H.L.,Federal University of Fluminense | Lilenbaum W.,Federal University of Fluminense | Machado G.,University of Minnesota | And 4 more authors.
Microbial Pathogenesis | Year: 2017

Leptospirosis is a cosmopolitan infectious disease that causes severe reproductive disorders in cattle, especially those related to abortion. This disease has rodents as main reservoirs; however, cattle are responsible for maintenance of the disease. Thus, the aim of this study was to identify the factors associated with infection and cause-effect relation of leptospirosis in dairy herds from Southern of Brazil. Serum samples of 1242 cows were collected from herds classified as of medium and high density, and tested by microscopic agglutination test (MAT). These farms were located in the West part of Santa Catarina State (Brazil). A total of 80 cows (6.44%) were considered positives for the infection with titration of 1:100. Using a multivariate analysis, we identified two factors associated to bovine leptospirosis: dog access to pastures (p < 0.001) and feed exposure to rodents (p = 0.05). Cause-effect analysis demonstrated that the occurrence of reproductive disorders was significantly (p = 0.01) linked to leptospirosis. Thus, we conclude that leptospirosis is prevalent in dairy cattle in the west part of Santa Catarina state, as well as the access of dogs to pastures and contact of rodents with feed increase the chance of cattle infection by Leptospira spp. © 2017 Elsevier Ltd


Campigotto G.,Santa Catarina State University | Volpato A.,Santa Catarina State University | Galli G.M.,Santa Catarina State University | Glombowsky P.,Santa Catarina State University | And 5 more authors.
Experimental Parasitology | Year: 2017

Many reproductive problems has been described in male and female animals infected by Trypanosoma evansi. Thus, the aim of this study was to evaluate the occurrence of vertical (Experiment I) and venereal (Experiment II) transmission of T. evansi in rats experimentally infected. In the experiment I, eight female Wistar rats were used: three animals as negative controls, and five rats were infected by T. evansi on day ten of gestation. Out of these eight females, half puppies were used for molecular analysis (polymerase chain reaction - PCR) for T. evansi. Two infected females showed delivery problems, such as stillbirth, and fetal death that also led to female death. Three female rats infected had normal delivery of stunted offspring at term that died 2 days after birth. Rats from the control group had normal delivery of healthy offspring. T. evansi PCR was positive for 80% (12/15) of pups in the infected group. For the experiment II, five male rats were infected by T. evansi, and monitored by blood smears to check the parasitemia level. When the male rats showed parasitemia between 2 and 5 parasites per field, they were individually housed with one female adult rat. After approximately 21 days, the females delivered their offspring. Blood sample was collected from the females for blood smears and T. evansi PCR tests, which revealed negative results. Therefore, we were able to prove the occurrence of transplacental transmission of T. evansi and its negative effect on female rats and their offspring. © 2017 Elsevier Inc.


Simoes R.R.,Federal University of Santa Maria | Simoes R.R.,Federal University of Santa Catarina | Coelho I.S.,Federal University of Santa Catarina | Junqueira S.C.,Federal University of Santa Catarina | And 8 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance The Condalia buxifolia root bark infusion is used in traditional medicine in Brazil as antipyretic, anti-inflammatory and against dysentery. This study was designed to investigate whether the methanolic extract of the root bark of Condalia buxifolia (MECb) exhibits antinociceptive and anti-inflammatory effects in mice. Furthermore, also was investigated the involvement of glutamatergic and opioidergic system in the antinociceptive effect induced by MECb. Materials and methods The antinociceptive and anti-inflammatory effects of intra-gastric gavage (i.g.) administered MECb (10-300 mg/kg) were evaluated in mice subjected to chemical (formalin, acetic-acid, glutamate) or thermal (hot plate) models of pain. The involvement of opioid system in the antinociceptive effect of the MECb was investigated in formalin test. Furthermore, a nonspecific effect of MECb was evaluated by measuring locomotor activity and exploratory behavior in open field test. Finally, was performed a phytochemical analysis of MECb. Results The phytochemical analysis of MECb was performed through HPLC analysis showing that the alkaloid Condaline-A is the main constituent. The intragastric administration of MECb (100-300 mg/kg) significantly inhibited the nociception caused by acetic acid (48±2%), inflammatory phase (49±3%) and paw edema (32±6) caused by formalin, and MECb (100 mg/kg, i.g.) also inhibited nociception caused by glutamate (41±7%). In addition, MECb (100-300 mg/kg, i.g.) increased the paw withdrawal latency in hot-plate test, without affecting the locomotor activity and exploratory behavior in open field test. Finally, the antinociceptive effects of MECb (100 mg/kg, i.g.) were significantly reversed by naloxone (1 mg/kg, i.p.) in the formalin test. Conclusion These data show, for the first time, that MECb has significant antinociceptive and anti-inflammatory effects, which appear to be related to the inhibition of the glutamatergic system and the activation of opioid mechanism, besides present central effects. These results support the use of Condalia buxifolia in traditional medicine and demonstrate that this plant has therapeutic potential for the development of phytomedicines with antinociceptive and anti-inflammatory properties. © 2015 Elsevier Ireland Ltd. All rights reserved.


Heimfarth L.,Federal University of Rio Grande do Sul | da Silva Ferreira F.,Federal University of Rio Grande do Sul | Pierozan P.,Federal University of Rio Grande do Sul | Mingori M.R.,Federal University of Rio Grande do Sul | And 3 more authors.
Toxicology | Year: 2017

Diphenylditelluride (PhTe)2 is a neurotoxin that disrupts cytoskeletal homeostasis. We are showing that different concentrations of (PhTe)2 caused hypophosphorylation of glial fibrillary acidic protein (GFAP), vimentin and neurofilament subunits (NFL, NFM and NFH) and altered actin organization in co-cultured astrocytes and neurons from cerebral cortex of rats. These mechanisms were mediated by N-methyl-D-aspartate (NMDA) receptors without participation of either L-type voltage-dependent calcium channels (L-VDCC) or metabotropic glutamate receptors. Upregulated Ca2+ influx downstream of NMDA receptors activated Ca2+-dependent protein phosphatase 2B (PP2B) causing hypophosphorylation of astrocyte and neuron IFs. Immunocytochemistry showed that hypophosphorylated intermediate filaments (IF) failed to disrupt their organization into the cytoskeleton. However, phalloidin-actin-FITC stained cytoskeleton evidenced misregulation of actin distribution, cell spreading and increased stress fibers in astrocytes. βIII tubulin staining showed that neurite meshworks are not altered by (PhTe)2, suggesting greater susceptibility of astrocytes than neurons to (PheTe)2 toxicity. These findings indicate that signals leading to IF hypophosphorylation fail to disrupt the cytoskeletal IF meshwork of interacting astrocytes and neurons in vitro however astrocyte actin network seems more susceptible. Our findings support that intracellular Ca2+ is one of the crucial signals that modulate the action of (PhTe)2 in co-cultured astrocytes and neurons and highlights the cytoskeleton as an end-point of the neurotoxicity of this compound. Cytoskeletal misregulation is associated with cell dysfunction, therefore, the understanding of the molecular mechanisms mediating the neurotoxicity of this compound is a matter of increasing interest since tellurium compounds are increasingly released in the environment. © 2017 Elsevier B.V.


Heimfarth L.,Federal University of Rio Grande do Sul | da Silva Ferreira F.,Federal University of Rio Grande do Sul | Pierozan P.,Federal University of Rio Grande do Sul | Loureiro S.O.,Federal University of Rio Grande do Sul | And 4 more authors.
Biochimica et Biophysica Acta - General Subjects | Year: 2016

Background Diphenylditelluride (PhTe)2 is a potent neurotoxin disrupting the homeostasis of the cytoskeleton. Methods Cultured astrocytes and neurons were incubated with (PhTe)2, receptor antagonists and enzyme inhibitors followed by measurement of the incorporation of [32P]orthophosphate into intermediate filaments (IFs). Results (PhTe)2 caused hyperphosphorylation of glial fibrillary acidic protein (GFAP), vimentin and neurofilament subunits (NFL, NFM and NFH) from primary astrocytes and neurons, respectively. These mechanisms were mediated by N-methyl-D-aspartate (NMDA) receptors, L-type voltage-dependent calcium channels (L-VDCCs) as well as metabotropic glutamate receptors upstream of phospholipase C (PLC). Upregulated Ca2 + influx activated protein kinase A (PKA) and protein kinase C (PKC) in astrocytes causing hyperphosphorylation of GFAP and vimentin. Hyperphosphorylated (IF) together with RhoA-activated stress fiber formation, disrupted the cytoskeleton leading to altered cell morphology. In neurons, the high intracellular Ca2 + levels activated the MAPKs, Erk and p38MAPK, beyond PKA and PKC, provoking hyperphosphorylation of NFM, NFH and NFL. Conclusions Our findings support that intracellular Ca2 + is one of the crucial signals that modulate the action of (PhTe)2 in isolated cortical astrocytes and neurons modulating the response of the cytoskeleton against the insult. General significance Cytoskeletal misregulation is associated with neurodegeneration. This compound could be a valuable tool to induce molecular changes similar to those found in different pathologies of the brain. © 2016 Elsevier B.V.


da Silva D.S.,University Federal Of Pelotasrs | da Silva C.E.H.,University Federal Of Pelotasrs | Soares M.S.P.,University Federal Of Pelotasrs | Azambuja J.H.,University Federal Of Pelotasrs | And 6 more authors.
European Journal of Medicinal Chemistry | Year: 2016

The present study assessed the biological potential of fourteen 1,3-thiazolidin-4-ones evaluating the antiglioma effect through decreasing of cell viability of glioblastoma multiform cells. The new compounds were efficient synthesized through multicomponent or multicomponent one-pot procedures in moderate to good yields (22–86%) from two arenealdehydes (4-(methylthio)benzaldehyde and 4-(methylsulfonyl)benzaldehyde), seven amines (aromatic and aliphatic) and mercaptoacetic acid. The compounds were identified and characterized by GC/MS and NMR, five of them by HRMS. Six thiazolidinones showed significant effect of decreasing cell viability compared to standard drug TMZ at 100 μM in 72 h in C6 cell line by MTT assay. The compounds 5b, 5e, 5g and 6e showed the best results in the screening at 100 μM and were analyzed at different concentrations (5, 25, 50, 100 and 250 μM). Compounds 5b and 5e showed statistical difference at 5 μM, 6e at 25 μM and 5g at 50 μM in 72 h of treatment. The cytotoxicity study in primary astrocytes cells was evaluated and none of fourteen compounds showed toxicity at 100 μM, eight of them were not cytotoxic at 250 μM, both in 72 h. In addition, the propidium iodide assay demonstrated that the compounds might induce cell death by necrosis. In conclusion, this work reports at least four compounds (5b, 5e, 5g and 6e) with potential anti-tumor effect against glioblastoma multiform cell presenting activity at low concentrations and safe profile of cytotoxicity. © 2016 Elsevier Masson SAS


Baldissera M.D.,Federal University of Santa Maria | Souza C.F.,Federal University of Santa Maria | Doleski P.H.,Federal University of Santa Maria | Junior G.B.,Federal University of Santa Maria | And 2 more authors.
Microbial Pathogenesis | Year: 2016

The aim of this study was to evaluate the cholinergic (acetylcholinesterase (AChE) and butyrylcholinesterase (BChE)) and purinergic (adenosine deaminase (ADA)) systems in head kidney, spleen, total blood and serum samples in experimentally infected fish with A. hydrophila, and the involvement of these systems during the inflammatory process. Silver catfish (Rhamdia quelen) juveniles were divided into two groups with seven fish each: uninfected (negative control) and infected (positive control). On day 2 post-infection, animals were euthanized and the head kidney, spleen, total blood and serum were collected. AChE and ADA activities in head kidney and spleen decreased in infected animals compared to uninfected animals, as well as AChE in total blood and seric ADA activities. BChE activity was not expressed in the evaluated tissues. Therefore, our results lead to the hypothesis that cholinergic and purinergic systems play an important role on the immune response against A. hydrophila with an anti-inflammatory effect. In summary, AChE and ADA activities reduced probably in order to protect against tissue inflammatory damage caused by infection. © 2016 Elsevier Ltd


Dalla Nora M.,University Federal Of Santa Mariars | Horner R.,University Federal Of Santa Mariars | de Carli D.M.,Servico Of Gastroenterologia Do Hospital Universitario | da Rocha M.P.,Servico Of Patologia Do Hospital Universitario | And 3 more authors.
Arquivos de Gastroenterologia | Year: 2016

Background - The diagnosis of H. pylori infection can be performed by non-invasive and invasive methods. The identification through a fecal antigen test is a non-invasive, simple, and relatively inexpensive test. Objective - To determine the diagnostic performance of fecal antigen test in the identification of H. pylori infection. Methods - H. pylori antigens were identified in the stools of dyspeptic patients undergoing upper gastrointestinal endoscopy. For the identification of H. pylori antigen, we use ImmunoCard STAT! HpSA with immunochromatography technique. Histopathology plus urease test were the gold standard. Results - We studied 163 patients, 51% male, mean age of 56.7± 8.5years. H. pylori infection was present in 49%. Fecal test presented: sensitivity 67.5% (CI95% 60.6-72.9); specificity 85.5% (CI95% 78.9-90.7); positive predictive value 81.8% (CI95% 73.4-88.4) and negative predictive value 73,2% (CI95% 67.5-77.6); Positive likelihood ratio was 4.7 (CI95% 2.9-7.9) and Negative Likelihood Ratio 0.4 (CI95% 0.3-0.5). The prevalence odds ratio for a positive test was 12.3 (CI95% 5.7-26.3). The index kappa between FAT and histology/urease test was 0.53 (CI95% 0.39-0.64). Conclusion - Immunochromatographic FAT is less expensive than the other methods and readily accepted by the patients but its diagnostic performance does not recommend its use in the primary diagnosis, when the patient may have an active infection. © 2016, Arquivos de Gastroenterologia. All rights reserved.


Sartor L.R.,Federal Technological University of Paraná | Lopes L.,Federal Technological University of Paraná | Martin T.N.,University Federal Of Santa Mariars | Ortiz S.,Federal Technological University of Paraná
Bioscience Journal | Year: 2015

Agrosilvipastoris systems are expanding the spaces occupied and gaining new adherents in order expansions productive in the same areas. But some issues, such as allelopathic effects of some cultures, in which case the pine, are still poorly known. This research aimed to evaluate the allelopathic effect of aqueous extract from the pine needles on the germination and development of lettuce, corn and beggarticks seedlings. The experiment was carried in a completely randomized design with treatments arranged in a factorial (2x5), where: conditions of the needles (green and dry) x extracts concentrations (0, 25, 50, 75 and 100%) in four replications, under temperature, humidity and light controlled. Evaluations were carried out time intervals of 24 hours, from 0 to 144 hours. It was evaluated the germination, average speed of germination, the length of the primary roots of lettuce and the length from the seedlings roots of lettuce, beggarticks and corn. Extracts the base of green needles affected the variables, where the effect increased with increase in concentration, the effect being more marked in beggarticks. In the statement the basis of dry pine needles, there were minimal results when compared to control. © 2015, Universidade Federal de Uberlandia. All rights reserved.

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