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João Pessoa, Brazil

Luciano M.N.,Laboratory of Pharmaceutical Technology | Ribeiro T.P.,Laboratory of Pharmaceutical Technology | Franca-Silva M.S.,Laboratory of Pharmaceutical Technology | Do Nascimento R.J.B.,Laboratory of Pharmaceutical Technology | And 8 more authors.
Journal of Cardiovascular Pharmacology | Year: 2011

The aim of this study was to investigate the mechanisms underlying the vasorelaxant effect induced by the polyphenolic compounds found in red wine from Vale do Saõ Francisco. In phenylephrine (10 1/4M) precontracted mesenteric artery rings, the red wine caused a concentration-dependent relaxation (maximum response to phenylephrine 10 1/4M ≤ 87.5% ± 6.5%, n ≤ 10). After endothelium removal, the vasorelaxant effect elicited by red wine was attenuated (28.4% ± 4.9%, n ≤ 10). In addition, the vasorelaxant effect induced by red wine in rings pretreated with 100 1/4M of N-nitro-l-arginine methyl ester and 10 1/4M of 1H-[1,2,4] oxadiazolo-[4,3-a]- quinoxalin-1-one was attenuated (23.4% ± 5.1%, n ≤ 7 and 11.8% ± 2.7%, n ≤ 6, respectively). Pretreatment with atropine did not affect the vasorelaxant effect induced by red wine (81% ± 3.9%, n ≤ 6). Furthermore, in rabbit aortic endothelial cell line, red wine 100 and 300 1/4g/mL caused concentration-dependent increases in nitric oxide levels (58 ± 1; 82 ± 7.9; "% of fluorescence, n ≤ 5, respectively). In conclusion, we suggest that the alcohol free-lyophilized red wine induces an endothelium-dependent vasorelaxant effect due, at least in part, to a secondary increase in the concentration of nitric oxide and that this effect might be associated with phenolic compounds found in the red wine. © 2011 by Lippincott Williams & Wilkins.

Rodrigues-Mascarenhas S.,University Federal da Parayba | Rodrigues-Mascarenhas S.,University Federal da Parayba | De Vasconcelos D.I.B.,University Federal da Parayba | De Vasconcelos D.I.B.,University Federal da Parayba | And 7 more authors.
Mediators of Inflammation | Year: 2011

Ouabain, an inhibitor of the Na +/K +-ATPase pump, was identified as an endogenous substance of human plasma. Ouabain has been studied for its ability to interfere with various regulatory mechanisms. Despite the studies portraying the ability of ouabain to modulate the immune response, little is known about the effect of this substance on the inflammatory process. The aim of this work was to study the effects triggered by ouabain on inflammation and nociceptive models. Ouabain produced a reduction in the mouse paw edema induced by carrageenan, compound 48/80 and zymosan. This anti-inflammatory potential might be related to the inhibition of prostaglandin E2, bradykinin, and mast-cell degranulation but not to histamine. Ouabain also modulated the inflammation induced by concanavalin A by inhibiting cell migration. Besides that, ouabain presented antinociceptive activity. Taken these data together, this work demonstrated, for the first time, that ouabain presented in vivo analgesic and anti-inflammatory effects. Copyright © 2011 Danielle Ingrid Bezerra de Vasconcelos et al.

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