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Zorzatto C.,State University of Maringa | Chiari L.,Embrapa Gado de Corte | De Araujo Bitencourt G.,University Estadual Of Sao Paulo Julio Of Mesquita Filho | Do Valle C.B.,Embrapa Gado de Corte | And 3 more authors.
Plant Breeding

A bulked segregant analysis using RAPD technique was carried out to identify molecular markers linked to apomixis in a Brachiaria humidicola F1 population that segregated 1 : 1 for the mode of reproduction (apomixis and sexual). A marker related to the apo-locus was found. Segregation data, together with this marker were used to generate a map of the region. This marker was located at 4.61 cM of the target locus, and it can be used in deploying marker-assisted selection for mode of reproduction in the hybrid progenies of this species. © 2010 Blackwell Verlag GmbH. Source

Pereira S.S.,University Estadual Of Sao Paulo Julio Of Mesquita Filho | Guimaraes F.C.M.,Embrapa Soja | Carvalho J.F.C.,Embrapa Soja | Stolf-Moreira R.,State University Londrina | And 5 more authors.
Genetics and Molecular Research

To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed their expression levels under drought stress. The NCBI software was used to find the predicted amino acid sequences of the transcription factors, and the Clustal X software was used to align soybean and other plant species sequences. Phylogenetic trees were built using the Mega 4.1 software by neighbor joining and the degree of confidence test by Bootstrap. Expression level studies were carried out using hydroponic culture; the experiments were designed in completely randomized blocks with three repetitions. The blocks consisted of two genotypes, MG/BR46 Conquista (drought-tolerant) and BR16 (drought-sensitive) and the treatments consisted of increasingly long dehydration periods (0, 25, 50, 75, and 100 min). The transcription factors presented domains and/or conserved regions that characterized them as belonging to the bzip, c2h2, myb, and nac families. Based on the phylogenetic trees, it was found that the myb, bzip and nac genes are closely related to myb78, bzip48 and nac2 of soybean and that c2h2 is closely related to c2h2 of Brassica napus. Expression of all genes was in general increased under drought stress in both genotypes. Major differences between genotypes were due to the lowering of the expression of the mybj7 and c2h2 genes in the drought-tolerant variety at some times. Over-expression or silencing of some of these genes has the potential to increase stress tolerance. © FUNPEC-RP. Source

Rendon M.Y.,University of Campinas | Gratao P.L.,University Estadual Of Sao Paulo Julio Of Mesquita Filho | Salva T.J.G.,Instituto Agronomico Of Campinas | Azevedo R.A.,University of Sao Paulo | Bragagnolo N.,University of Campinas
European Food Research and Technology

The development of the germination process and drought stress during the drying of coffee can generate reactive oxygen species, which can be neutralized by way of antioxidant mechanisms. No studies related to antioxidant enzymes during the drying of coffee were found in the literature, and considering their importance, the enzymatic activities of superoxide dismutase (SOD), guaiacol peroxidase (GPOX) and glutathione reductase (GR), and also the hydrogen peroxide content were evaluated during the drying of two types of coffee bean, one processed as natural coffee and the other as pulped natural coffee. The results showed a reduction in the SOD, GPOX and GR enzymatic activities of the natural coffee as compared to the pulped natural coffee during the drying period. Moreover, the hydrogen peroxide content of the natural coffee was greater than that of the pulped natural coffee. These results suggest the development of oxidative stress during the coffee drying process, controlled more efficiently in pulped natural coffee by the early action of GPOX during the drying process. Nevertheless, differential responses by SOD isoenzymes and possibly the role of other peroxidases also appear to be involved in the responses observed. © 2013 Springer-Verlag Berlin Heidelberg. Source

Carneiro F.S.,University Estadual Of Sao Paulo Julio Of Mesquita Filho
Genetics and molecular research : GMR

The Neotropical tree Hymenaea courbaril, locally known as Jatobá, is a valuable source of lumber and also produces comestible and medicinal fruit. We characterized Mendelian inheritance, linkage and genotypic disequilibrium at nine microsatellite loci isolated from H. courbaril, in order to determine if they would provide accurate estimates of population genetic parameters of this important Amazon species. The study was made on 250 open-pollinated offspring originated from 14 seed trees. Only one of nine loci presented significant deviation from the expected Mendelian segregation (1:1). Genotypic disequilibrium between pairwise loci was investigated based on samples from 55 adult and 56 juvenile trees. No genetic linkage between any paired loci was observed. After Bonferroni's corrections for multiple tests, we found no evidence of genotypic disequilibrium between pairs of loci. We conclude that this set of loci can be used for genetic diversity/ structure, mating system, gene flow, and parentage analyses in H. courbaril populations. Source

Vieira N.A.,University of Sao Paulo | Borgo H.C.,University of Sao Paulo | Dalben G.S.,University of Sao Paulo | Bachega M.I.,University of Sao Paulo | Pereira P.C.M.,University Estadual Of Sao Paulo Julio Of Mesquita Filho
Brazilian Journal of Microbiology

This study isolated and quantified intestinal bacteria of children with cleft palate before and after palatoplasty. A prospective study was conducted from May 2007 to September 2008 on 18 children with cleft palate, aged one to four years, of both genders, attending a tertiary cleft center in Brazil for palatoplasty, to analyze the effect of surgical palate repair on the concentration of anaerobes Bacteroides sp, Bifidobacterium sp and microaerophiles Lactobacillus sp in feces of infants with cleft palate before and 24 hours after treatment with cefazolin for palatoplasty. There was significant reduction of Lactobacillus sp (p < 0.002), Bacteroides sp (p < 0.001) and Bifidobacterium sp (p = 0.021) after palatoplasty, revealing that surgery and utilization of cefazolin significantly influenced the fecal microbiota comparing collections before and after surgery. However, due to study limitations, it was not possible to conclude that other isolated factors, such as surgical stress, anesthetics and other medications used in palatoplasty might have a significant influence on the microbiota. Considering the important participation of the intestinal microbiota on both local and systemic metabolic and immunological activities of the host, professionals should be attentive to the possible influence of these changes in patients submitted to cleft repair. © 2013, Sociedade Brasileira de Microbiologia. Source

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