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Fernando de Noronha (Distrito Estadual), Brazil

De Souza L.C.,State University of Maringa | Zambom M.A.,University Estadual do Oeste do Parana | Gundt S.,University Estadual do Oeste do Parana | Pasqualotto M.,University Estadual do Oeste do Parana | And 3 more authors.
Bioscience Journal | Year: 2015

This study aimed to determine the chemical composition and degradability of forage and byproducts agroindustries commonly used in West Region Paraná. The foods studied were Sudan grass, the hay alfalfa, oats, ryegrass and Tifton 85, and the leucaena, titônia, wet waste cassava starch (RSFM), sugar cane yeast, wet brewery’s grain (RUC) compound®, corn grits, cookies residue and noodlesresidue. Foods were analyzed for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and in vitro dry matter digestibility (IVDMD), cell wall (IVDCW) food collected in the region. From of characteristics analyzed was possible to group into voluminous foods, sudan grass, leucaena, titônio, Tifton hay, oats, ryegrass and alfalfa, and RUFM; energy foods: compond®, corn grits, cookies residue and noodles residue; protein foods: yeast and RUC. All foods studied have potential for use in ruminant feed, however more studies are necessary to define the criteria for the use of these foods dairy herd, within each category. © 2015, Biosci. J. All right reserved.

Spositto F.L.E.,State University of Maringa | Campanerut P.A.Z.,University Estadual Of Maringapr | Ghiraldi L.D.,University Estadual Of Maringapr | Leite C.Q.F.,Sao Paulo State University | And 4 more authors.
Brazilian Journal of Microbiology | Year: 2014

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species. © 2014, Sociedade Brasileira de Microbiologia.

Antonelli-Ushirobira T.M.,State University of Maringa | Blainski A.,State University of Maringa | Fernandes H.G.,State University of Maringa | Moura-Costa G.F.,State University of Maringa | And 7 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, popularly known as baicuru, has been used in folk medicine to treat menstrual cramps and to regulate menstrual periods. However, little is known about its safety. This study evaluated the safety through in vivo tests of the acute, long-term, and liver toxicity, and the mutagenicity of the crude extract (CE) from rhizomes of L. brasiliense. Materials and methods The acute toxicity was assessed in Swiss mice, and the chronic toxicity in Wistar rats. Male and female mice received the CE orally in single doses of 1.0, 2.0, 3.0, 4.0, or 5.0 g/kg. Clinical changes and mortality rate were used as parameters to assess the acute toxicity. In the long-term evaluation, male and female Wistar rats were treated orally with daily doses of the CE (50, 100, or 200 mg/kg) for 90 days. Assessments of weight, behavior and food intake, urinalysis, biochemical and hematological analyses, as well as macro- and microscopic observations of several organs were performed. The redox state of the liver was evaluated as a means of investigating the liver toxicity, and the micronucleus test to assess mutagenicity was also performed. Results Evaluation of acute toxicity indicated no apparent clinical change in the animals; the LD50 was 4.8 g/kg. Evaluation after 90 days administration showed that the CE, even in higher doses than are considered therapeutic, appeared to be safe. The micronucleus test demonstrated a low mutagenic potential for the CE. Conclusion Our results showed that treatment with the CE from L. brasiliense caused low or no toxicity, as assessed using these doses and evaluation methods. © 2015 Elsevier Ireland Ltd.All rights reserved.

Felismino M.F.,Federal University of Mato Grosso do Sul | Maior R.L.S.,University Estadual Of Maringapr | Damasceno G.A.,Federal University of Mato Grosso do Sul | Pott A.,Federal University of Mato Grosso do Sul | Pagliarini M.S.,University Estadual Of Maringapr
Genetics and Molecular Research | Year: 2015

This is the first report of meiotic division in Urochloa adspersa (Trin.) collected from the Brazilian Chaco. Meiotic analyses were performed on three specimens of U. adspersa named G10, G15, and G16. Inflorescences were collected and fixed in a mixture of ethanol and acetic acid (3:1, v/v) for 24 h and then stored in 70% alcohol. Diakinesis revealed different chromosome numbers and ploidy levels. All three plants were polyploids: G10 and G15 exhibited 2n = 6x = 54 chromosomes (arranged in 27 bivalents), while G16 exhibited 2n = 4x = 36 chromosomes (18 bivalents). Meiotic behavior was mainly normal in the hexaploid G15 and the tetraploid G16 (5.3 and 6.2% of the cells were abnormal, respectively), revealing only a few meiotic abnormalities that are common to polyploids, i.e., those related to irregular chromosome segregation. G10 exhibited other meiotic abnormalities during meiosis II, such as chromosome stickiness, irregular spindle orientation, and irregular cytokinesis, which led to the formation of a few triads, resulting in 16.9% of the cells being abnormal. The origin of these abnormalities is discussed, and we suggest that the genes that control meiotic steps may be present in the Urochloa gene pool. © FUNPEC-RP.

da Costa A.R.,UNICESUMAR | Rezende R.,University Estadual Of Maringapr | de Freitas P.S.L.,University Estadual Of Maringapr | Andrade Goncalves A.C.,University Estadual Of Maringapr | Frizzone J.A.,University of Sao Paulo
IRRIGA | Year: 2015

The objective of this study was to evaluate growth and production of Italian zucchini plants under different levels of nitrogen and potassium by fertigation. The experiment was conducted in a greenhouse in the Technical Center of Irrigation at Maringá State University, PR. Seedling transplanting to the experimental area was carried out 21 days after sowing in polystyrene trays. Seedlings were transplanted to soil using 1.0 m spacing between rows and 0.7 m between plants. Targeted irrigation using a drip emitter pipe was used in each plant. A completely randomized and factorial design (4 × 4) was used with three replicates. The treatments consisted of different Nitrogen (0 kg ha-1 N, 90 kg ha-1 N, 180 kg ha-1 N, 270 kg ha-1 N) and Potassium (0 kg ha-1 K2O, 90 kg ha-1 K2O, 180 kg ha-1 K2O, 270 kg ha-1 K2O) levels. The following growth and production parameters were evaluated: plant height, stalk diameter and fruit set index and yield. Nitrogen and Potassium fertigation improved the growth and production parameters, which make it an efficient tool to provide nutrients for the Italian zucchini. © 2015, Universidade Estadual Paulista - UNESP. All rights reserved.

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