Fernando de Noronha (Distrito Estadual), Brazil
Fernando de Noronha (Distrito Estadual), Brazil

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Machado S.A.,University Estadual Of Maringapr | Oliveira A.V.,State University of Maringá | Fabrin T.M.C.,University Estadual Of Maringapr | Prioli S.M.A.P.,State University of Maringá | Prioli A.J.,State University of Maringá
Genetics and Molecular Research | Year: 2016

The pteridophytes Salvinia minima, S. herzogii, and S. auriculata are among the most abundant aquatic macrophytes in the upper Paraná River floodplain. Since some species have highly similar morphological features, it is very difficult to identify members of this genus to the species level. An indication of this difficulty is a set of poorly differentiated taxa comprising S. auriculata and S. herzogii known as the ‘S. auriculata complex’, which is found in the Paraná River together with other Salvinia species such as S. biloba and S. molesta. Some authors have reported the existence of inter-species hybrids. Despite the complex Salvinia taxonomy, few genetic studies have been performed on purported species within the genus to resolve this complexity. The present study was conducted to determine useful molecular sequences for the discrimination of Salvinia species of the upper Paraná River floodplain. Molecular data were compared with data of other species of the genus to clarify phylogenetic relationships, employing the nucleotide sequence trnL-trnF from the chloroplast DNA. The results revealed that Salvinia populations in the upper Paraná River floodplain belong to different species and indicated that species of the S. auriculata complex may be distinguished from one another after the division of the S. minima group, corroborating results by other researchers. Although the taxonomic position of S. oblongifolia was clarified, as high closeness between S. oblongifolia and the S. auriculata complex was reported, Salvinia kinship is still not thoroughly established and further investigations in morphology and molecular diversity are required. © 2016 The Authors.


Spositto F.L.E.,State University of Maringá | Campanerut P.A.Z.,University Estadual Of Maringapr | Ghiraldi L.D.,University Estadual Of Maringapr | Leite C.Q.F.,São Paulo State University | And 4 more authors.
Brazilian Journal of Microbiology | Year: 2014

We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species. © 2014, Sociedade Brasileira de Microbiologia.


Eguchi E.S.,Mato Grosso State University | Cecato U.,State University of Maringá | Muniz A.S.,University Estadual Of Maringapr | de Oliveira C.A.L.,State University of Maringá | And 2 more authors.
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2015

The objective of this study was to evaluate the productive and chemical characteristics of Marandu grass fertilized with poultry manure with or without the use of soil chiseling, during a period of 210 days. The experiment was conducted in a Dark-Red Latosol (Haplustox) of sandy texture, in a randomized block design with 4 replicates, in a 6 x 2 factorial scheme (0, 1.037, 2.074, 4.148, 6.222 t ha-1 of poultry manure and an additional treatment with chemical fertilizer based on 2.074 t ha-1, with and without soil chiseling). Grass-cutting management was performed between 95% of light interception by the canopy and the residual height of 0.15 m. The application of poultry manure resulted in an increase of forage production with higher number of cuts (NC). A linear model described the effect of the applied manure doses on dry matter accumulation (DMA), stems + sheaths (SS) and dead material (DM). There was significant interaction between manure doses and soil managements for leaf blade (LB), with greater variations in the treatment without soil chiseling. For plant chemical evaluation, a negative effect of poultry manure was observed on the contents of Ca, Mg, N, S, Mn and Zn, which may be due to the increased number of cuts. Poultry manure positively changed the production of Marandu grass. © 2015, Departamento de Engenharia Agricola - UFCG/Cnpq. All rights reserved.


De Souza L.C.,State University of Maringá | Zambom M.A.,University Estadual do Oeste do Parana | Gundt S.,University Estadual do Oeste do Parana | Pasqualotto M.,University Estadual do Oeste do Parana | And 3 more authors.
Bioscience Journal | Year: 2015

This study aimed to determine the chemical composition and degradability of forage and byproducts agroindustries commonly used in West Region Paraná. The foods studied were Sudan grass, the hay alfalfa, oats, ryegrass and Tifton 85, and the leucaena, titônia, wet waste cassava starch (RSFM), sugar cane yeast, wet brewery’s grain (RUC) compound®, corn grits, cookies residue and noodlesresidue. Foods were analyzed for dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF) and in vitro dry matter digestibility (IVDMD), cell wall (IVDCW) food collected in the region. From of characteristics analyzed was possible to group into voluminous foods, sudan grass, leucaena, titônio, Tifton hay, oats, ryegrass and alfalfa, and RUFM; energy foods: compond®, corn grits, cookies residue and noodles residue; protein foods: yeast and RUC. All foods studied have potential for use in ruminant feed, however more studies are necessary to define the criteria for the use of these foods dairy herd, within each category. © 2015, Biosci. J. All right reserved.


Antonelli-Ushirobira T.M.,State University of Maringá | Blainski A.,State University of Maringá | Fernandes H.G.,State University of Maringá | Moura-Costa G.F.,State University of Maringá | And 7 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Limonium brasiliense (Boiss.) Kuntze, Plumbaginaceae, popularly known as baicuru, has been used in folk medicine to treat menstrual cramps and to regulate menstrual periods. However, little is known about its safety. This study evaluated the safety through in vivo tests of the acute, long-term, and liver toxicity, and the mutagenicity of the crude extract (CE) from rhizomes of L. brasiliense. Materials and methods The acute toxicity was assessed in Swiss mice, and the chronic toxicity in Wistar rats. Male and female mice received the CE orally in single doses of 1.0, 2.0, 3.0, 4.0, or 5.0 g/kg. Clinical changes and mortality rate were used as parameters to assess the acute toxicity. In the long-term evaluation, male and female Wistar rats were treated orally with daily doses of the CE (50, 100, or 200 mg/kg) for 90 days. Assessments of weight, behavior and food intake, urinalysis, biochemical and hematological analyses, as well as macro- and microscopic observations of several organs were performed. The redox state of the liver was evaluated as a means of investigating the liver toxicity, and the micronucleus test to assess mutagenicity was also performed. Results Evaluation of acute toxicity indicated no apparent clinical change in the animals; the LD50 was 4.8 g/kg. Evaluation after 90 days administration showed that the CE, even in higher doses than are considered therapeutic, appeared to be safe. The micronucleus test demonstrated a low mutagenic potential for the CE. Conclusion Our results showed that treatment with the CE from L. brasiliense caused low or no toxicity, as assessed using these doses and evaluation methods. © 2015 Elsevier Ireland Ltd.All rights reserved.


da Costa A.R.,UNICESUMAR | Rezende R.,University Estadual Of Maringapr | de Freitas P.S.L.,University Estadual Of Maringapr | Andrade Goncalves A.C.,University Estadual Of Maringapr | Frizzone J.A.,University of Sao Paulo
IRRIGA | Year: 2015

The objective of this study was to evaluate growth and production of Italian zucchini plants under different levels of nitrogen and potassium by fertigation. The experiment was conducted in a greenhouse in the Technical Center of Irrigation at Maringá State University, PR. Seedling transplanting to the experimental area was carried out 21 days after sowing in polystyrene trays. Seedlings were transplanted to soil using 1.0 m spacing between rows and 0.7 m between plants. Targeted irrigation using a drip emitter pipe was used in each plant. A completely randomized and factorial design (4 × 4) was used with three replicates. The treatments consisted of different Nitrogen (0 kg ha-1 N, 90 kg ha-1 N, 180 kg ha-1 N, 270 kg ha-1 N) and Potassium (0 kg ha-1 K2O, 90 kg ha-1 K2O, 180 kg ha-1 K2O, 270 kg ha-1 K2O) levels. The following growth and production parameters were evaluated: plant height, stalk diameter and fruit set index and yield. Nitrogen and Potassium fertigation improved the growth and production parameters, which make it an efficient tool to provide nutrients for the Italian zucchini. © 2015, Universidade Estadual Paulista - UNESP. All rights reserved.


Bredow C.,University Estadual Of Maringapr | Azevedo J.L.,University of Sao Paulo | Pamphile J.A.,University Estadual Of Maringapr | Mangolin C.A.,University Estadual Of Maringapr | Rhoden S.A.,Intituto Federal Catarinense
Genetics and Molecular Research | Year: 2015

Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size, and availability of databases for comparison. The objective of this study was to analyze endophytic bacterial diversity in agricultural crops using published papers, sequence databases, and phylogenetic analysis. Fourteen papers were selected in which the ribosomal 16S rRNA gene was used to identify endophytic bacteria, in important agricultural crops, such as coffee, sugar cane, beans, corn, soybean, tomatoes, and grapes, located in different geographical regions (America, Europe, and Asia). The corresponding 16S rRNA gene sequences were selected from the NCBI database, aligned using the Mega 5.2 program, and phylogenetic analysis was undertaken. The most common orders present in the analyzed cultures were Bacillales, Enterobacteriales, and Actinomycetales and the most frequently observed genera were Bacillus, Pseudomonas, and Microbacterium. Phylogenetic analysis showed that only approximately 1.56% of the total sequences were not properly grouped, demonstrating reliability in the identification of microorganisms. This study identified the main genera found in endophytic bacterial cultures from plants, providing data for future studies on improving plant agriculture, biotechnology, endophytic bacterium prospecting, and to help understand relationships between endophytic bacteria and their interactions with plants. © FUNPEC-RP.


Orlandelli R.C.,University Estadual Of Maringapr | de Almeida T.T.,University Estadual Of Maringapr | Alberto R.N.,University Estadual Of Maringapr | Polonio J.C.,University Estadual Of Maringapr | And 2 more authors.
Brazilian Journal of Microbiology | Year: 2015

Endophytes are being considered for use in biological control, and the enzymes they secrete might facilitate their initial colonization of internal plant tissues and direct interactions with microbial pathogens. Microbial proteases are also biotechnologically important products employed in bioremediation processes, cosmetics, and the pharmaceutical, photographic and food industries. In the present study, we evaluated antagonism and competitive interactions between 98 fungal endophytes and Alternaria alternata, Colletotrichum sp., Phyllosticta citricarpa and Moniliophthora perniciosa. We also examined the proteolytic activities of endophytes grown in liquid medium and conducted cup plate assays. The results showed that certain strains in the assemblage of P. hispidum endophytes are important sources of antifungal properties, primarily Lasiodiplodia theobromae JF766989, which reduced phytopathogen growth by approximately 54 to 65%. We detected 28 endophytes producing enzymatic halos of up to 16.40 mm in diameter. The results obtained in the present study highlight the proteolytic activity of the endophytes Phoma herbarum JF766995 and Schizophyllum commune JF766994, which presented the highest enzymatic halo diameters under at least one culture condition tested. The increased activities of certain isolates in the presence of rice or soy flour as a substrate (with halos up to 17.67 mm in diameter) suggests that these endophytes have the potential to produce enzymes using agricultural wastes. © 2015, Sociedade Brasileira de Microbiologia.


Felismino M.F.,Federal University of Mato Grosso do Sul | Maior R.L.S.,University Estadual Of Maringapr | Damasceno G.A.,Federal University of Mato Grosso do Sul | Pott A.,Federal University of Mato Grosso do Sul | Pagliarini M.S.,University Estadual Of Maringapr
Genetics and Molecular Research | Year: 2015

This is the first report of meiotic division in Urochloa adspersa (Trin.) collected from the Brazilian Chaco. Meiotic analyses were performed on three specimens of U. adspersa named G10, G15, and G16. Inflorescences were collected and fixed in a mixture of ethanol and acetic acid (3:1, v/v) for 24 h and then stored in 70% alcohol. Diakinesis revealed different chromosome numbers and ploidy levels. All three plants were polyploids: G10 and G15 exhibited 2n = 6x = 54 chromosomes (arranged in 27 bivalents), while G16 exhibited 2n = 4x = 36 chromosomes (18 bivalents). Meiotic behavior was mainly normal in the hexaploid G15 and the tetraploid G16 (5.3 and 6.2% of the cells were abnormal, respectively), revealing only a few meiotic abnormalities that are common to polyploids, i.e., those related to irregular chromosome segregation. G10 exhibited other meiotic abnormalities during meiosis II, such as chromosome stickiness, irregular spindle orientation, and irregular cytokinesis, which led to the formation of a few triads, resulting in 16.9% of the cells being abnormal. The origin of these abnormalities is discussed, and we suggest that the genes that control meiotic steps may be present in the Urochloa gene pool. © FUNPEC-RP.

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