Santo André, Brazil
Santo André, Brazil

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Fukuda T.Y.,Setor de Fisioterapia | Fukuda T.Y.,Federal University of São Paulo | Jesus J.F.,Setor de Fisioterapia | Santos M.G.,Setor de Fisioterapia | And 4 more authors.
Revista Brasileira de Fisioterapia | Year: 2010

Background: Despite the increase in the use of low-level laser therapy (LLLT), there is still a lack of consensus in the literature regarding how often the equipment must be calibrated. Objective: To evaluate the real average power of LLLT devices in the Greater São Paulo area. Methods: For the evaluation, a LaserCheck power meter designed to calibrate continuous equipment was used. The power meter was programmed with data related to the laser's wavelength to gauge the real average power being emitted. The LLLT devices were evaluated in two ways: fi rst with the device cooled down and then with the device warmed up for 10 minutes. For each condition, three tests were performed. The laser probe was aligned with the power meter, which provided the real average power being emitted by the LLLT device. All of the data and information related to the laser application were collected with the use of a questionnaire fi lled in by the supervising therapists. Results: The 60 devices evaluated showed defi cit in real average power in the cooled-down and warmedup condition. The statistical analysis (ANOVA) showed a signifi cant decrease (p<0.05) in the real average power measured in relation to the manufacturer's average power. On average, the most common dose in the clinics was 4 J/cm2, and the most desired effects were healing and anti-infl ammatory effects. According to the World Association for Laser Therapy (WALT), 1 to 4 J of fi nal energy are necessary to achieve these effects, however only one device was able to reach the recommended therapeutic window. Conclusion: The LLLT devices showed a defi cit in real average power that emphasized a lack of order in the application of this tool. The present study also showed the need for periodical calibration of LLLT equipment and a better technical knowledge of the therapists involved.


Smith L.B.,Institute Botanica | Tavares A.R.,Institute Botanica | Kanashiro S.,Institute Botanica | Lima R.F.G.,Institute Botanica | And 3 more authors.
Acta Horticulturae | Year: 2013

Bromeliads are widespread in Brazil, especially at the Atlantic Forest and their cultivation is also an economically viable alternative for floriculture. The current investigation aimed to understand the in vitro growth and shooting of the bromeliad Neoregelia marmorata, as a tool for its preservation and commercial production. Plantlets of N. marmorata obtained from in vitro germinated seeds, were cultured on MS media added with different concentrations of auxins and cytokinins. The experimental design was entirely randomized, comprising of 12 treatments with NAA (Naphthalene Acetic Acid - 0; 1.34 or 2.69 μM) x BAP (6-benzylaminopurine - 0; 2.22; 4.44 or 8.87 μM) as well as 12 treatments with NAA (0; 1.34 or 2.69 μM) x TDZ (Thidiazuron - 0; 0.57; 1.14 or 2.27 μM). Increasing concentrations of BAP (2.22, 4.44 or 8.87 μM) in combination with 2.69 μM NAA produced the highest number of shoots. TDZ did not show any positive response regarding the number of shoots, in fact probably that the concentrations used were phytotoxic. NAA in conjunction with 2.22 or 4.44 μM BAP was also important in increasing the number of shoots, once again showing the importance of this auxin is shoot induction when associated with BAP.


Fukuda T.Y.,Federal University of São Paulo | Tanji M.M.,University of Sao Paulo | Tanji M.M.,University do Grande | Jesus J.F.,University do Grande | And 3 more authors.
Lasers in Surgery and Medicine | Year: 2010

Background and Objective: The results of low-level infrared laser (LLL) systemic action on inflammatory modulation process, specifically diminishing proinflammatory and producing anti-inflammatory cytokines are extremely controversial in the literature. More studies are necessary to clarify the biomodulation process. The main objective was to investigate the effect of a single session of an AsGaAl laser on spleen cells interleukin-6 (IL-6) and tumor necrosis factor - alpha (TNF-α) release, in vivo, in mice. Study Design/Materials and Methods: In a pilot study, 18 isogenic mice were distributed in three groups: control (no surgical procedure, n = 6), sham (surgical procedure with three standard cutaneous incisions, followed by abdominal muscle incision followed by suture, n = 6) and LLL (same procedure followed by a single LLL exposure 12 hours after the procedure, n = 6). The animals in the LLL group received a single infrared continuous laser session (780 nm wavelength, power of 20 mW, energy density of 10 J/cm 2) on three points (20 seconds per point), and final energy of 0.4 J. All animals of the sham and LLL groups were sacrificed 36 hours after surgical procedure; the spleen mononuclear cells were isolated and cultivated for 48 hours. The IL-6 and TNF-α were measured by the ELISA method. Results: IL-6 and TNF-α concentrations released by the mononuclear cells showed significant differences between the control and sham group (P<0.07). However, there were no differences between the control and LLL group and between the sham and LLL groups (P>0.07). Conclusion: The single session of infrared LLL showed a tendency of decreasing the IL-6 and TNF-α release by mononuclear spleen cells in mice after application, although there was not a significant difference between the sham and LLL group. Conclusions regarding effectiveness of a single session procedure cannot be made due to the low statistical power of this pilot study. © 2010 Wiley-Liss, Inc.


Schlesinger D.,University of Sao Paulo | Schlesinger D.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein | Grinberg L.T.,University of Sao Paulo | Grinberg L.T.,Memory and Aging Center | And 15 more authors.
Molecular Psychiatry | Year: 2013

Previous studies in dementia epidemiology have reported higher Alzheimer's disease rates in African-Americans when compared with White Americans. To determine whether genetically determined African ancestry is associated with neuropathological changes commonly associated with dementia, we analyzed a population-based brain bank in the highly admixed city of São Paulo, Brazil. African ancestry was estimated through the use of previously described ancestry-informative markers. Risk of presence of neuritic plaques, neurofibrillary tangles, small vessel disease, brain infarcts and Lewy bodies in subjects with significant African ancestry versus those without was determined. Results were adjusted for multiple environmental risk factors, demographic variables and apolipoprotein E genotype. African ancestry was inversely correlated with neuritic plaques (P0.03). Subjects with significant African ancestry (n112, 55.4%) showed lower prevalence of neuritic plaques in the univariate analysis (odds ratio (OR) 0.72, 95% confidence interval (CI) 0.55-0.95, P0.01) and when adjusted for age, sex, APOE genotype and environmental risk factors (OR 0.43, 95% CI 0.21-0.89, P0.02). There were no significant differences for the presence of other neuropathological alterations. We show for the first time, using genetically determined ancestry, that African ancestry may be highly protective of Alzheimer's disease neuropathology, functioning through either genetic variants or unknown environmental factors. Epidemiological studies correlating African-American race/ethnicity with increased Alzheimer's disease rates should not be interpreted as surrogates of genetic ancestry or considered to represent African-derived populations from the developing nations such as Brazil. © 2013 Macmillan Publishers Limited.


Jaimes R.F.V.V.,University do Grande | Afonso M.L.C.d.A.,Technological and Nuclear Institute of Portugal | Rogero S.O.,Brazilian Nuclear Energy Research Institute (IPEN) | Agostinho S.M.L.,University of Sao Paulo | Barbosa C.A.,Villares Metals SA
Materials Letters | Year: 2010

Nickel, a component of stainless steels (SS) applied in orthopedic implants may cause allergic processes in human tissues. P558 nickel free SS was studied to verify its viability as a substitute for stainless steel containing nickel. Its performance is compared to ISO 5832-9 and F138 most used nowadays grades in implants fabrications, in minimum essential medium, MEM, at 37 °C. Potentiodynamic polarization curves, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and "in vitro" cytotoxicity were used as techniques. From the electrochemical point of view P558 SS is comparable to ISO 5832-9 SS in MEM. It remains passivated until the transpassivation potential, above which generalized corrosion occurs. F138 presents pitting corrosion at 370 mV/SCE. The cytotoxicity results showed that P558, ISO 5832-9 and F138 do not present cytotoxic character. Therefore, these results suggest that P558 SS can be applied in orthopedic implants. © 2010 Elsevier B.V. All rights reserved.


Jaimes R.F.V.V.,University do Grande | Afonso M.L.C.A.,ITN | Rogero S.O.,Brazilian Nuclear Energy Research Institute (IPEN) | Barbosa C.A.,Villares Metals SA | And 2 more authors.
Revista Escola de Minas | Year: 2010

The electrochemical behavior of ISO 5832-9 stainless steel at 37 °C in 0.9% NaCl, Ringer Lactate and minimum essential medium (MEM) has been studied, using linear voltammetry, and surface analysis by SEM and EDS. Mechanical and toxicity tests were made. ISO 5832-9 is passivated at corrosion potential (Ecorr) and it does not present pitting corrosion on the media studied from Ecorr to 50 mV above the transpassivation potential (Ei). SEM and EDS analysis have shown that the sample previously immersed in MEM presents a different behavior at 50 mV above Ei: the manganese oxide inclusions are absent in the surface. Ecorr values and passivation current density values jpass changed according to the following. The stainless steel was characterized as non toxic in the cytotoxicity assay.


PubMed | University do Grande and University of Sao Paulo
Type: Journal Article | Journal: Revista latino-americana de enfermagem | Year: 2014

analyze contextual aspects of the beginning of tobacco use in different social groups, from everyday representations about the act of smoking.five focus groups were conducted to promote discussion about the context of beginning of tobacco use, with groups of people who represented different patterns of social reproduction. The data analysis was based on the theory of social representations, which contextualizes how each group presents the tobacco consumption.the contexts of the beginning of tobacco use were diverse, according to patterns of social reproduction; there were common representations to all groups, but there were also unique representations of each social group. Tobacco is represented as indispensable for groups in unstable social reproduction situations, and as an instrument of pleasure and stress relief for those who can access other material assets.the study contributed to exposing the concepts on tobacco consumption that are socially disseminated, which can serve as an instrument to planning programs and health actions.


Dos Santos Fernandes J.P.,Mackenzie Presbyterian University | Savino G.,Mackenzie Presbyterian University | Amarante A.C.G.,Mackenzie Presbyterian University | De Sousa M.R.,University Camilo Castelo Branco | And 4 more authors.
Quimica Nova | Year: 2013

Parabens are p-hydroxybenzoic acid esters widely used as preservatives. With the aim of teaching the structure-activity relationships (SAR) knowledge in a practical form, this paper proposed a practical class to view the SAR of parabens as antimicrobial agents. Methyl, ethyl, n-propyl, isopropyl and isopentyl paraben compounds were synthesized and their respective antimicrobial activities were assessed through determination of minimum inhibitory concentrations (MIC) against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 stains. With the MIC values, it was possible to verify theircorrelation with calculated lipophilicity (ClogP). This method can be applied in practical Medicinal Chemistry classes.


This qualitative study aimed to identify difficulties experienced by Community Health Agents (CHA) in the course of daily practice of care. Data were collected from semi-structured interviews with twelve CHA from four Basic Health Units in a city of the state of São Paulo, Brazil. From an analysis of the speech of subjects, we found that the deficiency of health services, the workload of the nurses responsible for the team and the service rejection by users make it difficult to implement the health actions. This research emphasizes the limitations expressed by the health agents, and points towards the necessity of an evolving discussion of this theme, seeking strategies that enable the consolidation of community health principles.

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