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Santo André, Brazil

Smith L.B.,Institute Botanica | Tavares A.R.,Institute Botanica | Kanashiro S.,Institute Botanica | Lima R.F.G.,Institute Botanica | And 3 more authors.
Acta Horticulturae | Year: 2013

Bromeliads are widespread in Brazil, especially at the Atlantic Forest and their cultivation is also an economically viable alternative for floriculture. The current investigation aimed to understand the in vitro growth and shooting of the bromeliad Neoregelia marmorata, as a tool for its preservation and commercial production. Plantlets of N. marmorata obtained from in vitro germinated seeds, were cultured on MS media added with different concentrations of auxins and cytokinins. The experimental design was entirely randomized, comprising of 12 treatments with NAA (Naphthalene Acetic Acid - 0; 1.34 or 2.69 μM) x BAP (6-benzylaminopurine - 0; 2.22; 4.44 or 8.87 μM) as well as 12 treatments with NAA (0; 1.34 or 2.69 μM) x TDZ (Thidiazuron - 0; 0.57; 1.14 or 2.27 μM). Increasing concentrations of BAP (2.22, 4.44 or 8.87 μM) in combination with 2.69 μM NAA produced the highest number of shoots. TDZ did not show any positive response regarding the number of shoots, in fact probably that the concentrations used were phytotoxic. NAA in conjunction with 2.22 or 4.44 μM BAP was also important in increasing the number of shoots, once again showing the importance of this auxin is shoot induction when associated with BAP.

Fukuda T.Y.,Setor de Fisioterapia | Fukuda T.Y.,Federal University of Sao Paulo | Jesus J.F.,Setor de Fisioterapia | Santos M.G.,Setor de Fisioterapia | And 4 more authors.
Revista Brasileira de Fisioterapia | Year: 2010

Background: Despite the increase in the use of low-level laser therapy (LLLT), there is still a lack of consensus in the literature regarding how often the equipment must be calibrated. Objective: To evaluate the real average power of LLLT devices in the Greater São Paulo area. Methods: For the evaluation, a LaserCheck power meter designed to calibrate continuous equipment was used. The power meter was programmed with data related to the laser's wavelength to gauge the real average power being emitted. The LLLT devices were evaluated in two ways: fi rst with the device cooled down and then with the device warmed up for 10 minutes. For each condition, three tests were performed. The laser probe was aligned with the power meter, which provided the real average power being emitted by the LLLT device. All of the data and information related to the laser application were collected with the use of a questionnaire fi lled in by the supervising therapists. Results: The 60 devices evaluated showed defi cit in real average power in the cooled-down and warmedup condition. The statistical analysis (ANOVA) showed a signifi cant decrease (p<0.05) in the real average power measured in relation to the manufacturer's average power. On average, the most common dose in the clinics was 4 J/cm2, and the most desired effects were healing and anti-infl ammatory effects. According to the World Association for Laser Therapy (WALT), 1 to 4 J of fi nal energy are necessary to achieve these effects, however only one device was able to reach the recommended therapeutic window. Conclusion: The LLLT devices showed a defi cit in real average power that emphasized a lack of order in the application of this tool. The present study also showed the need for periodical calibration of LLLT equipment and a better technical knowledge of the therapists involved.

This qualitative study aimed to identify difficulties experienced by Community Health Agents (CHA) in the course of daily practice of care. Data were collected from semi-structured interviews with twelve CHA from four Basic Health Units in a city of the state of São Paulo, Brazil. From an analysis of the speech of subjects, we found that the deficiency of health services, the workload of the nurses responsible for the team and the service rejection by users make it difficult to implement the health actions. This research emphasizes the limitations expressed by the health agents, and points towards the necessity of an evolving discussion of this theme, seeking strategies that enable the consolidation of community health principles.

Schlesinger D.,University of Sao Paulo | Schlesinger D.,Instituto Israelita Of Ensino E Pesquisa Albert Einstein | Grinberg L.T.,University of Sao Paulo | Grinberg L.T.,Memory and Aging Center | And 15 more authors.
Molecular Psychiatry | Year: 2013

Previous studies in dementia epidemiology have reported higher Alzheimer's disease rates in African-Americans when compared with White Americans. To determine whether genetically determined African ancestry is associated with neuropathological changes commonly associated with dementia, we analyzed a population-based brain bank in the highly admixed city of São Paulo, Brazil. African ancestry was estimated through the use of previously described ancestry-informative markers. Risk of presence of neuritic plaques, neurofibrillary tangles, small vessel disease, brain infarcts and Lewy bodies in subjects with significant African ancestry versus those without was determined. Results were adjusted for multiple environmental risk factors, demographic variables and apolipoprotein E genotype. African ancestry was inversely correlated with neuritic plaques (P0.03). Subjects with significant African ancestry (n112, 55.4%) showed lower prevalence of neuritic plaques in the univariate analysis (odds ratio (OR) 0.72, 95% confidence interval (CI) 0.55-0.95, P0.01) and when adjusted for age, sex, APOE genotype and environmental risk factors (OR 0.43, 95% CI 0.21-0.89, P0.02). There were no significant differences for the presence of other neuropathological alterations. We show for the first time, using genetically determined ancestry, that African ancestry may be highly protective of Alzheimer's disease neuropathology, functioning through either genetic variants or unknown environmental factors. Epidemiological studies correlating African-American race/ethnicity with increased Alzheimer's disease rates should not be interpreted as surrogates of genetic ancestry or considered to represent African-derived populations from the developing nations such as Brazil. © 2013 Macmillan Publishers Limited.

Jaimes R.F.V.V.,University do Grande | Afonso M.L.C.d.A.,Technological and Nuclear Institute of Portugal | Rogero S.O.,Brazilian Nuclear Energy Research Institute (IPEN) | Agostinho S.M.L.,University of Sao Paulo | Barbosa C.A.,Villares Metals SA
Materials Letters | Year: 2010

Nickel, a component of stainless steels (SS) applied in orthopedic implants may cause allergic processes in human tissues. P558 nickel free SS was studied to verify its viability as a substitute for stainless steel containing nickel. Its performance is compared to ISO 5832-9 and F138 most used nowadays grades in implants fabrications, in minimum essential medium, MEM, at 37 °C. Potentiodynamic polarization curves, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and "in vitro" cytotoxicity were used as techniques. From the electrochemical point of view P558 SS is comparable to ISO 5832-9 SS in MEM. It remains passivated until the transpassivation potential, above which generalized corrosion occurs. F138 presents pitting corrosion at 370 mV/SCE. The cytotoxicity results showed that P558, ISO 5832-9 and F138 do not present cytotoxic character. Therefore, these results suggest that P558 SS can be applied in orthopedic implants. © 2010 Elsevier B.V. All rights reserved.

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