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Gohar M.,University Diagnostic Laboratory | Sheikh A.A.,University Diagnostic Laboratory | Anjum A.A.,University Diagnostic Laboratory | Hussain T.,University of Veterinary and Animal Sciences | And 4 more authors.
Journal of Animal and Plant Sciences | Year: 2015

Confirmed E. coli (n = 25) were analyzed for the presence of plasmids and cured using temperature and sodium dodecyl sulphate (SDS) treatments. Plasmid profiling of the isolates showed that 96% isolates were harboring plasmids of varying size and number. The average plasmid number among the isolates was 2.28, ranging from 0-5 plasmids per isolate and plasmid size varied from 100bp-12kb which was distributed randomly in these isolates. Statistically no significant correlation (p>0.05) was found between antibiotic resistance and plasmid profiles. Plasmid curing study showed 3.9% and 16.7% curing efficiency with that of temperature and SDS treatment, respectively. Statistical analysis revealed that resistant isolates, treated with either physical or chemical treatments produced significant results (p<0.05) of plasmid curing. Antimicrobial resistance genes might be located on plasmid and statistically no relationship was observed between antibiotic resistance and plasmid profiles. © 2015, Pakistan Agricultural Scientists Forum. All Rights Reserved.

Shabbir M.Z.,University Diagnostic Laboratory | Rabbani M.,University Diagnostic Laboratory | Yaqub T.,University Diagnostic Laboratory | Ahmad A.,University Diagnostic Laboratory | And 2 more authors.
Pakistan Journal of Zoology | Year: 2010

Toxocariosis is a parasitic zoonosis and has worldwide distribution affecting dogs and cats. This retrospective study describes the comparative clinical epidemiology of toxocariosis in dogs and cats according to feeding pattern, sex, age and different seasons in and around Lahore. A total of 1874 fecal samples consisting of 1203 dogs (64.19%) and 671 cats (35.80%) were examined through fecal floatation technique, A total of 270 dogs (22.44%) and 198 cats (29.50%) were found positive to T. canis and T cati, respectively. The significant incidence of Toxocara was recorded in cats (P = 0.0007) as compared to dogs. The study revealed that Toxocara incidence is high in uncontrolled fed population (P = 0.0001), more in young puppies (P = 0.000) and kittens (P = 0.014), and sex dependent (P = 0.000) with higher percentage in queens (45.70) and male dogs (49.46). Season wise, a significant difference in both dogs (P = 0.0000) and cats (P = 0. 001) was observed with higher prevalence in summer (32.93%, 40.1%) followed by spring (20.95%, 28%), autumn (23.46%, 23.94%) and winter (15.16%, 21.96%).Copyright 2010 Zoological Society of Pakistan.

Sohail M.U.,Government of Pakistan | Ijaz A.,University of Veterinary and Animal Sciences | Younus M.,University of Veterinary and Animal Sciences | Shabbir M.Z.,University Diagnostic Laboratory | And 7 more authors.
Journal of Applied Poultry Research | Year: 2013

A study was conducted to elucidate the effects of supplementation of mannan oligosaccharide (MOS), a prebiotic, and probiotic mixture (PM) on growth performance, relative weights (RW) of viscera, and population of selected intestinal bacteria to broilers reared under cyclic heat stress (HS). Two hundred fifty 1-d-old broiler chicks were randomly divided into 5 treatment groups with 5 replicates. From d 22 onward, the broilers were reared either in a thermoneutral zone (TN) or exposed to HS until d 42. Birds were fed either a corn-based basal diet (TN and HS groups) or the same diet supplemented with 0.5% MOS, 0.1% PM, or their combination as synbiotic. Birds were killed on d 42 to collect viscera and jejunal and cecal digesta. Digesta was used to enumerate Clostridium perfringens, total coliforms, and Escherichia coli. The HS decreased mean BW gain, feed consumption, and FE of broilers compared with the TN broilers. The TN group had higher RW of spleen, bursa of Fabricius, and ceca compared with the HS group. The supplementation of PM and SYN improved mean BW gain and FE compared with the HS group. Increase in RW of spleen, bursa, intestine, and ceca was observed in the supplemented groups compared with the HS group. Based on these findings, we concluded that HS has significant negative effects on mean RW of viscera and growth performance of broilers. Moreover, feeding a diet supplemented with either MOS or PM alone or as combination can partially or completely ameliorate these effects. © 2013 Poultry Science Association, Inc.

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