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David R.O.,University of Alcala | David R.O.,Complutense University of Madrid | Gonzalez-Munoz M.J.,University of Alcala | Benedi J.,University Complutese Of Madrid | And 2 more authors.
European Journal of Lipid Science and Technology

The origin and presence of triglyceride polymers in small intestine have been poorly studied. The present study combined a short in vivo absorption experiment and high-performance size-exclusion chromatography determination. Groups of six male Wistar rats were administered by esophageal probe 1 g/100 g body weight unused palm olein and palm oleins used in 40 and 90 potato frying operations. Small intestines were dissected, cleaned of luminal fat, and analyzed for the presence of triglyceride polymers (oligomers and/or dimers) after 4 h oil administration. The intestinal fat content did not change but the polymers content was positively and signicantly correlated (r = 0.5983; p<0.01) with the amount of polymers present in the oil tested. The small intestine contained 5.05 mg [median and percentile 25 (1.57 mg)-percentile 75 (10.40 mg)] of polymers after 4-h exposure to palm olein used for frying 90 times. The results suggested that 2.7-4.9% of the triglyceride polymers administered were present in the small intestine 4 h after ingestion. TBARS levels (p<0.05) and the redox index (oxidized glutathione/total glutathione) (p<0.01) in the small intestine increased signicantly after exposure to the palm olein used in 90 frying operations. In conclusion, administration of altered oil increased the presence of resynthesized polymers in the small intestine, thus contributing to small intestine oxidative stress. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Fesharaki O.,University Complutese Of Madrid | Arribas J.,University Complutese Of Madrid | Arribas J.,Complutense University of Madrid | Lopez Martinez N.,University Complutese Of Madrid
Journal of Iberian Geology

The western area of the Cenozoic Madrid Basin has not been adequately studied. This, combined with the high homogeneity of detrital facies makes the stratigraphic correlation with other areas of the basin rather difficult. Consequently, only a detailed characterization of different study zones can allow subsequent correlation over this area. Over the last years there have been discovered several vertebrate fossil sites in this area that allow the dating of the sediments which host the fossil remains and provide data about palaeoclimatic trends. In this paper we present the results of the light minerals petrographic analysis carried out in one of these sites (Somosaguas paleontological site). Previous palaeontological and isotopic studies in this site indicate a climate event of cooling and rising aridity that has been described globally for the period after the Miocene Climatic Optimum. The petrographic data and indices presented here corroborate this trend towards a more arid climate through the Somosaguas sedimentary succession. Besides we study the grades of alteration of plagioclase grains as a proxy in the evaluation of palaeoclimatic variations. The increase towards the top of the succession of less altered plagioclase grains suggests a decrease in precipitations and thus more aridity. Part of the quartz and K-feldspar grains display several features like embayments and alterations pointing to palaeosoils formation and reworking processes. These characteristics and other observations suggest several sedimentary pulses in a geotectonic setting of “basement uplift” and a mixed lithological provenance for the Somosaguas deposits (granites, gneisses and minor quantities of low-grade metamorphic rocks). © 2015, Universidad Complutense de Madrid. All Rights Reserved. Source

Zakka L.R.,Center for Blistering Diseases | Keskin D.B.,Dana-Farber Cancer Institute | Reche P.,University Complutese Of Madrid | Ahmed A.R.,Center for Blistering Diseases
Clinical and Experimental Immunology

In this report,we present 15 patients with histological and immunopathologically proven pemphigus vulgaris (PV). After a mean of 80 months since the onset of disease, when evaluated serologically, they had antibodies typical of PV and pemphigoid (Pg). Similarly, 18 patients with bullous pemphigoid (BP) and mucous membrane pemphigoid (MMP) were diagnosed on the basis of histology and immunopathology.After a mean of 60 months since the onset of disease, when their sera were evaluated they were found to have Pg and PV autoantibodies. In both groups of patients the diseases were characterized by a chronic course, which included several relapses and recurrences and were non-responsive to conventional therapy. The major histocompatibility complex class II (MHC II) genes were studied in both groups of patients and phenotypes associated typically with them were observed. Hence, in 33 patients, two different pathogenic autoantibodies were detected simultaneously. The authors provide a computer model to show that each MHC II gene has relevant epitopes that recognize the antigens associated with both diseases. Using the databases in these computer models, the authors present the hypothesis that these two autoantibodies are produced simultaneously due to the phenomena of epitope spreading. © 2010 The Authors Clinical and Experimental Immunology © 2010 British Society for Immunology. Source

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