Mogi das Cruzes, Brazil
Mogi das Cruzes, Brazil

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PubMed | University of Santiago de Compostela, University of Vigo, São Paulo State University, University Braz Cubas and 2 more.
Type: Journal Article | Journal: Acta stomatologica Croatica | Year: 2016

The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real-time reverse transcriptase reaction.The sample included exfoliative cytology from the oral cavity mucosa of patients with no visible clinical changes, using Orcellex Rovers BrushTrizol group revealed higher average concentration, followed by Direct-zolConsidering all aspects, concentration, purity and time spent in the procedures, the Direct-zol


Motta L.J.,Odontopediatria | Bachiega J.C.,Nove de Julho University | Guedes C.C.,University Braz Cubas | Laranja L.T.,Odontopediatria | Bussadori S.K.,Nove de Julho University
Clinics | Year: 2011

OBJECTIVE: To determine whether there is a correlation between halitosis and mouth breathing in children. STUDY DESIGN: Fifty-five children between 3 and 14 years of age were divided into two groups (nasal and mouth breathing) for the assessment of halitosis. A descriptive analysis was conducted on the degree of halitosis in each group. The chi-square test was used for comparison between groups, with a 5% level of significance. RESULTS: There was a significantly greater number of boys with the mouth-breathing pattern than girls. A total of 23.6% of the participants had no mouth odor, 12.7% had mild odor, 12.7% had moderate odor and 50.9% had strong odor. There was a statistically significant association between halitosis and mouth breathing. CONCLUSIONS: The occurrence of halitosis was high among the children evaluated, and there was a statistically significant association between halitosis and mouth breathing. © 2011 CLINICS.


Lino J.L.S.,University Braz Cubas | Christ B.,Institute Estudos Avancados IEAV CTA
Chinese Journal of Physics | Year: 2012

In the present paper we study the Schwinger multichannel (SMC) method with plane waves as a basis set (SMC-PW) for the elastic integral cross sections of He, Ne, H 2, and CH 4 by positron impact and also the inelastic integral cross sections of H 2 (transition X 1Σ g +→ B 1Σ u +). The essence of this work is based on the fact that the main limitation of the SMC method for positron scattering resides in what makes it a general method, i.e., the expansion of the scattering function is done in an L 2 basis (Cartesian Gaussian functions) and this is very effective for short range potentials. Our effort is to improve the SMC method through the use of plane waves as trial functions (SMC-PW). In order to do this, we have to evaluate reliable codes involving matrix elements of the type 〈 →kf |V G o (+)V |k i → which is here done by direct numerical quadrature. We find that the 〈 →kf |V G o (+)V ||ki → term is adequate when a minimum number of points is used for the radial and angular Gauss-Legendre quadratures. These quadratures are recommended for the efficient calculation of elastic positron scattering by atoms and molecules. We also present a preliminary study on the positron-impact electronic excitation of H 2 as investigated also by Arretche and Lima [Phys. Rev. A 74, 042713 (2006)] using the SMC method in the range 13.5-30 eV. The propose of this work was to study the numerical stability of the SMC-PW and also investigate the influence of open channels (energetically accessible electronic states). Cross sections were performed for the elastic case at intermediate energies in the range 50-200 eV. The results (elastic and inelastic) are consistent with the experimental data and theoretical studies. © 2012 The Physical Society of the Republic of China.


Roman E.A.F.R.,University of Campinas | Reis D.,University Braz Cubas | Romanatto T.,University of Medical Sciences of Costa Rica | Maimoni D.,University Braz Cubas | And 5 more authors.
Molecular and Cellular Endocrinology | Year: 2010

Central leptin action requires PI3K activity to modulate glucose homeostasis and peripheral metabolism. However, the mechanism behind this phenomenon is not clearly understood. We hypothesize that hypothalamic PI3K activity is important for the modulation of the AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) pathway, PGC1α, and AKT in skeletal muscle (SM). To address this issue, we injected leptin into the lateral ventricle of rats. Hypothalamic JAK2 and AKT were activated by intracerebroventricular (ICV) injection of leptin in a time-dependent manner. Central leptin improved tolerance to glucose (GTT), increased PGC1α expression, and AKT, AMPK, ACC and JAK2 phosphorylation in the soleus muscle. Previous ICV administration of either LY294002 or propranolol (IP) blocked these effects. We concluded that the activation of the hypothalamic PI3K pathway is important for leptin-induced AKT phosphorylation, as well as for active catabolic pathway through AMPK and PGC1α in SM. Thus, a defective leptin signalling PI3K pathway in the hypothalamus may contribute to peripheral resistance to insulin associated to diet-induced obesity. © 2009 Elsevier Ireland Ltd. All rights reserved.


Corazza P.H.,University Of Passo Fundo | De Castro H.L.,University Braz Cubas | Feitosa S.A.,São Paulo State University | Kimpara E.T.,São Paulo State University | Della Bona A.,University Of Passo Fundo
American Journal of Dentistry | Year: 2015

Purpose: To investigate the influence of CAD-CAM diamond bur deterioration on surface roughness (Ra) and maximum failure load (Lf) of Y-TZP-based ceramic (YZ) substructures (SB) veneered with a feldspathic porcelain. Methods: Two sets of burs (B1 and B2) were used to fabricate 30 YZ SB each in a CAD/CAM system (Cerec InLab). The SB were identified (1-30) according to the milling sequence (MS). SEM images of the burs were recorded before milling, and after milling 15 and 30 SB. The SB Ra was measured. All SB were veneered, cemented onto a fiber reinforced epoxy resin die, and loaded to failure. Specimens from B1 group were cyclic fatigued (106 cycles) before loading to failure. Fractographic analysis was performed. Data were statistically analyzed using Student's t-test, Weibull analysis, Pearson's correlation and ANOVA (α= 0.05). Results: The mean Ra value of B1 specimens was statistically greater than B2. Weibull modulus of B1 and B2 were statistically similar. The correlation between MS and Lf was not statistically significant for the groups. MS and Ra had significant correlation for both groups (Bl: r= -0.514, P= 0.015; B2: r= -0.462, P= 0.03). Although the visual aspect (SEM) of the burs was similar after 30 millings, the mean Ra values were significantly different after 27 millings for Bl and 24 millings for B2.


Bussadori S.K.,Nove de Julho University | Guedes C.C.,University Braz Cubas | Bachiega J.C.,University Braz Cubas | Santis T.O.,Nove de Julho University | Motta L.J.,University Braz Cubas
Journal of Clinical Pediatric Dentistry | Year: 2011

Background: The chemical-mechanical removal of caries involves the chemical softening of the infected dentin, following by the mechanical removal of the softened tissue using non-cutting manual instruments. Papacárie® (Fórmula&Ação, Sao Paulo, Brazil) is a product in gel form used for the chemical-mechanical softening of the affected tissue. Objective: The purpose of the present study was to evaluate the effectiveness of Papacárie®. Methods: Clinical and radiographic evaluations were performed on 14 young permanent molars treated with Papacárie® and restored with glass ionomer cement (VitroMolar, DFL Ind. Com LTDA, Rio de Janeiro, Brazil) 24 months following intervention. Results: Success was achieved in 13 of the 14 cases. Conclusion: Papacárie® was effective in the treatment of carious lesions and constitutes a conservative alternative that offers benefits to patients.


PubMed | University Braz Cubas, São Paulo State University and Heliopolis Hospital
Type: | Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology | Year: 2016

The genotoxic impact of HIV infection on the oral cavity malignancies is unknown. The aim of this study was to evaluate the effect of HIV infection in micronucleus (MN) frequency on the oral mucosa of HIV+ patients and establish a relationship with early cytogenetic changes in oral carcinogenesis.Thirty HIV+ individuals who are under highly active antiretroviral therapy (HAART) and 30 non-HIV patients were evaluated. Two smears were taken from the lateral border of the tongue and mouth floor and stained by Feulgen. The frequency of MN was examined in 3000 cells per subject under common microscopy.MN analysis showed no significant difference between groups by Mann-Whitney U-test for total MNs (P = 0.178). The presence of single MN was greater in control group with statistical significance (P = 0.009), while in HIV group, multiple MNs were exhibited in higher mean.HIV patients under HAART therapy and low viral load values showed higher frequency of multiple MNs, which, although not statistically significant, may be caused by the action of the Vpr gene, an accessory gene of HIV. These results corroborate the theory of HIV infection cytogenetic damage.


PubMed | University Braz Cubas and São Paulo State University
Type: Journal Article | Journal: Brazilian dental journal | Year: 2016

Adenosquamous carcinoma is an aggressive variant of squamous cell carcinoma. This report describes a case of adenosquamous carcinoma with clinical features of a benign lesion and discusses the differential diagnoses, especially regarding histopathological and immunohistochemical analyses. A 45-year-old male was referred to our outpatient clinic complaining about a rapid-growing enlargement in hard palate. Clinical examination revealed an erythematous and pedunculated nodule with lobulated non-ulcerated surface. Excisional biopsy was performed following clinical diagnosis of pyogenic granuloma. Histologically, the specimen consisted of areas characterizing both well-differentiated squamous cell carcinoma and true adenocarcinoma. After a broad list of immunohistochemical markers was evaluated (AE1/AE3, CEA, CK5, CK7, CK8/18, p53, p63 and Ki67), the diagnosis of adenosquamous carcinoma was rendered and the patient referred to complementary surgery. Adenosquamous carcinoma represents a challenge in diagnostic routine due to its rarity, diverse range of clinical presentations and histological features. Furthermore, classical clinical benign features may be present in malignant lesions; hence the submission of every surgical specimen to histological analysis is mandatory to provide the patient the adequate treatment.


PubMed | University Braz Cubas, Sao Leopoldo Mandic Research Center and University of Sao Paulo
Type: Journal Article | Journal: The journal of contemporary dental practice | Year: 2016

Radiopacity is a fundamental requisite to check marginal adaptation of restorations. Our objective was to assess the radiopacity of 28 brands of light-cured composite resins and compare their radiopacity with that of enamel, dentin, and aluminum of equivalent thickness.Composite resin disks (0.2, 0.5, and 1 mm) were radiographed by the digital method, together with an aluminum penetrometer and a human tooth equivalent tooth section. The degree of radiopacity of each image was quantified using digital image processing. Wilcoxon nonparametric test was used for comparison of the mean thickness of each material.All of the materials tested had an equal or greater radiopacity than that of aluminum of equivalent thickness. Similar results for enamel were found with the exception of Durafill, which was less radiopaque than enamel (p < 0.05). All the specimens were more radiopaque than dentin, except for P90 (which was equally radiopaque) and Durafill (which was less radiopaque). The thickness of the specimens may influence the similarity to the enamels radiopacity. All of the composite resins comply with specification #27 of the American Dental Association. The radiopacity of Amelogen Plus, Aph, Brilhiante, Charisma, Concept Advanced, Evolux X, Exthet X, Inten S, Llis, Master Fill, Natural Look, Opallis, P60, Tetric, Tph, Z100, and Z250 was significantly higher than that of enamel (p < 0.05).With these composites, it is possible to observe the boundaries between restoration and tooth structure, thus allowing clinicians to establish the presence of microleakage or restoration gap.Suitable radiopacity is an essential requisite for good-quality esthetic restorative materials. We demonstrate that only some composites have the sufficient radiopacity to observe the boundaries between restoration and tooth structure, which is the main cause of restoration failure.


PubMed | University Braz Cubas, São Paulo State University and University of Sao Paulo
Type: | Journal: Archives of oral biology | Year: 2016

The aim of this study was to evaluate the effect of chronic smoking on the expression profile of the repair genes MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers and never smokers.The sample consisted of thirty exfoliative cytology smears per group obtained from Smokers and Never Smokers. Total RNA was extracted and expression of the MLH1, MSH2 and ATM genes were evaluated by quantitative real-time and immunocytochemistry. The gene and protein expression data were correlated to the clinical data. Gene expression was analyzed statistically using the Student t-test and Pearsons correlation coefficient, with p<0.05.MLH1, MSH2 and ATM genes were downregulated in the smoking group compared to the control with significant values for MLH1 (p=0.006), MSH2 (p=0.0001) and ATM (p=0.0001). Immunocytochemical staining for anti-MLH1, anti-MSH2 and anti-ATM was negative in Never Smokers; in Smokers it was rarely positive. No significant correlation was observed among the expression of MLH1, MSH2, ATM and age, number of cigarettes consumed per day, time of smoking during life, smoking history or levels of CO in expired air.The expression of genes and proteins related to DNA repair mechanism MLH1, MSH2 and ATM in the normal oral mucosa of chronic smokers was reduced.

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