Universities for Human Disease Cell Signalling and Regulation

Luzhou, China

Universities for Human Disease Cell Signalling and Regulation

Luzhou, China

Time filter

Source Type

Dai R.Y.,Luzhou Medical College | Dai R.Y.,Universities for Human Disease Cell Signalling and Regulation | Chen S.K.,Luzhou Medical College | Yan D.M.,Luzhou Medical College | And 7 more authors.
Folia Biologica | Year: 2010

The potential pro-survival role of phosphatidylinositol 3-kinase (PI3K)/Akt during endoplasmic reticulum stress has been well-characterized. However, the detailed mechanisms remain largely unknown. Here, we showed that PI3K/Akt inhibition promoted endoplasmic reticulum stress-induced apoptosis in a glucose-regulated protein 78 (GRP78)-dependent manner. During endoplasmic reticulum stress, high levels of Akt phosphorylation were sustained for at least 18 h in HEK293 cells. Importantly, PI3K/Akt enhanced GRP78 accumulation through increasing its stability following endoplasmic reticulum stress. Furthermore, Akt1, but not Akt2 or Akt3, was involved in GRP78 stability regulation. These results suggest that PI3K/Akt inhibits endoplasmic reticulum stress-induced apoptosis in HEK293 cells, at least in part, by promoting GRP78 protein stability.

Loading Universities for Human Disease Cell Signalling and Regulation collaborators
Loading Universities for Human Disease Cell Signalling and Regulation collaborators