Scholler P.,French National Center for Scientific Research |
Scholler P.,French Institute of Health and Medical Research |
Scholler P.,Universites Of Montpellier 1And2 |
Scholler P.,British Petroleum |
And 14 more authors.
Progress in Molecular Biology and Translational Science | Year: 2013
High-throughput screening requires easy-to-monitor, rapid, robust, reliable, and miniaturized methods to test thousands of compounds on a target in a short period, in order to find active drugs. Only a few methods have been proved to fulfill all these requirements. New screening approaches based on fluorescence and especially on the principle of resonance energy transfer are being developed to study one of the main targets in the pharmaceutical industry, namely, the G protein-coupled receptors (GPCRs). Two types of approaches are clearly defined: generic approaches that are immediately applicable to a lot of targets such as second messenger kits or kinase kits; target-specific approaches that sense the receptor itself such as fluorescent ligands or fluorescent partners. This chapter focuses on sensors and approaches using the time-resolved Förster resonance energy transfer and homogeneous time-resolved fluorescence principle, their use, and their prospective applications for screening drugs acting on GPCRs. © 2013 Elsevier Inc.
Wilkinson R.N.,University of Sheffield |
Jopling C.,French National Center for Scientific Research |
Jopling C.,French Institute of Health and Medical Research |
Jopling C.,Universites Of Montpellier 1And2 |
Van Eeden F.J.M.,University of Sheffield
Progress in Molecular Biology and Translational Science | Year: 2014
The zebrafish has been rapidly adopted as a model for cardiac development and disease. The transparency of the embryo, its limited requirement for active oxygen delivery, and ease of use in genetic manipulations and chemical exposure have made it a powerful alternative to rodents. Novel technologies like TALEN/CRISPR-mediated genome engineering and advanced imaging methods will only accelerate its use. Here, we give an overview of heart development and function in the fish and highlight a number of areas where it is most actively contributing to the understanding of cardiac development and disease. We also review the current state of research on a feature that we only could wish to be conserved between fish and human; cardiac regeneration. © 2014 Elsevier Inc.