Bielefeld, Germany
Bielefeld, Germany

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Burney S.,University Paris - Sud | Wenzel R.,Free University of Berlin | Kottke T.,Universitatsstrasse 25 | Roussel T.,University Paris - Sud | And 5 more authors.
Angewandte Chemie - International Edition | Year: 2012

A quick switch: A single amino acid substitution at a conserved residue (D396N) of Arabidopsis cryptochrome-1 (Atcry1) confers single-stranded DNA repair activity in vitro, conferring photolyase activity onto the cryptochrome (see graph). The mutant protein undergoes photoreduction of flavin to the fully reduced anionic form, similar to photolyases and unlike wild-type cryptochromes. © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Budke C.,Universitatsstrasse 25 | Budke C.,Bielefeld University | Dreyer A.,Universitatsstrasse 25 | Dreyer A.,TU Hamburg - Harburg | And 11 more authors.
Crystal Growth and Design | Year: 2014

Experimental investigations of ice recrystallization inhibition (IRI) efficacy have been performed for a large number of different substances, including natural antifreeze proteins (AFP) and antifreeze glycoproteins (AFGP), several synthetic AFGP analogues, as well as synthetic polymers. Here we define IRI efficacy as that concentration at which the ice recrystallization rate is dominated by the IRI compound. The investigated 39 compounds show IRI efficacies from about 2 mmol L-1 for the least effective compound still showing activity to about 1 nmol L-1, which corresponds to the highest efficacy found for natural AFGP samples. Hence, the assay employed allows for a quantitative comparison of IRI efficacy over a range of at least 6 orders of magnitude, thereby enabling studies of distinguishing effects induced by even subtle structural variations in AFGP analogues that were synthesized. Our results show that AFGP are by far the most effective IRI agents in our assay, and we surmise that this particular efficacy may be due to their disaccharide moieties. This supposition is supported by the fact that IRI efficacy is strongly reduced for monosaccharide AFGP analogues, as well as for AFGP analogues with acetyl-protected monosaccharide moieties. © 2014 American Chemical Society.

Shah A.B.,Bielefeld University | Schielzeth H.,Bielefeld University | Schielzeth H.,Friedrich - Schiller University of Jena | Albersmeier A.,Universitatsstrasse 25 | And 2 more authors.
Ecology and Evolution | Year: 2016

Despite recent advances in high-throughput sequencing, difficulties are often encountered when developing microsatellites for species with large and complex genomes. This probably reflects the close association in many species of microsatellites with cryptic repetitive elements. We therefore developed a novel approach for isolating polymorphic microsatellites from the club-legged grasshopper (Gomphocerus sibiricus), an emerging quantitative genetic and behavioral model system. Whole genome shotgun Illumina MiSeq sequencing was used to generate over three million 300 bp paired-end reads, of which 67.75% were grouped into 40,548 clusters within RepeatExplorer. Annotations of the top 468 clusters, which represent 60.5% of the reads, revealed homology to satellite DNA and a variety of transposable elements. Evaluating 96 primer pairs in eight wild-caught individuals, we found that primers mined from singleton reads were six times more likely to amplify a single polymorphic microsatellite locus than primers mined from clusters. Our study provides experimental evidence in support of the notion that microsatellites associated with repetitive elements are less likely to successfully amplify. It also reveals how advances in high-throughput sequencing and graph-based repetitive DNA analysis can be leveraged to isolate polymorphic microsatellites from complex genomes. © 2016 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Antoniadis V.,University of Thessaly | Shaheen S.M.,Kafr El Sheikh University | Shaheen S.M.,University of Wuppertal | Boersch J.,University of Wuppertal | And 5 more authors.
Journal of Environmental Management | Year: 2016

Although soil contamination by potentially toxic elements (PTEs) in Europe has a history of many centuries, related problems are often considered as having been dealt with due to the enforcement of tight legislations. However, there are many unsolved issues. We aimed to assess PTE levels in highly contaminated soils and in garden edible vegetables using human health risk indices in order to evaluate the availability and mobilization of arsenic (As), copper (Cu), manganese (Mn), mercury (Hg), lead (Pb), and zinc (Zn). In four gardens in Germany, situated on, or in the vicinity of, a mine dump area, we planted beans (Phaseolus vulgaris ssp. nanus), carrots (Daucus sativus) and lettuce (Lactuca sativa ssp. capitata). We examined soil-to-plant mobilization of elements using transfer coefficient (TC), as well as soil contamination using contamination factor (CF), enrichment factor (EF), and bioaccumulation index (I geo). In addition, we tested two human health risk assessment indices: Soil-induced hazard quotient (HQS) (representing the "direct soil ingestion" pathway), and vegetable-induced hazard quotient (HQV) (representing the "vegetable intake" pathway). The studied elements were highly elevated in the soils. The values in garden 2 were especially high (e.g., Pb: 13789.0 and Hg: 36.8 mg kg-1) and largely exceeded the reported regulation limits of 50 (for As), 40 (Cu), 400 (Pb), 150 (Zn), and 5 (Hg) mg kg-1. Similarly, element concentrations were very high in the grown vegetables. The indices of CF, EF and I geo were enhanced even to levels that are rarely reported in the literature. Specifically, garden 2 indicated severe contamination due to multi-element deposition. The contribution of each PTE to the total of measured HQS revealed that Pb was the single most important element causing health risk (contributing up to 77% to total HQS). Lead also posed the highest risk concerning vegetable consumption, contributing up to 77% to total HQV. The presence of lead in both cases was followed by that of As, Mn and Hg. We conclude that in multi-element contamination cases, along with high-toxicity elements (here, Pb, As and Hg) other elements may also be responsible for increasing human health risks (i.e., Mn), due to the possibility of adverse synergism of the PTEs. © 2016.

Kuck D.,Bielefeld University | Kuck D.,Universitatsstrasse 25 | Linke J.,Bielefeld University | Teichmann L.C.,Bielefeld University | And 6 more authors.
Physical Chemistry Chemical Physics | Year: 2016

The solid-state molecular structure of centrohexaindane (1), a unique hydrocarbon comprising six benzene rings clamped to each other in three dimensions around a neopentane core, and the molecular packing in crystals of 1·CHCl3 are reported. The molecular Td-symmetry and the Cartesian orientation of the six indane wings of 1 in the solid state have been confirmed. The course and limitation of electrophilic aromatic substitution of 1 are demonstrated for the case of nitration. Based on nitration experiments of a lower congener of 1, tribenzotriquinacene 5, the six-fold nitrofunctionalisation of 1 has been achieved in excellent yield, giving four constitutional isomers, two nonsymmetrical (14 and 17) and two C3-symmetrical ones (15 and 16), all of which contain one single nitro group in each of the six benzene rings. The relative yields of the four isomers (∼3:1:1:3) point to a random electrophilic attack of the electrophiles at the twelve formally equivalent outer positions of the aromatic periphery of 1, suggesting electronic independence of its six aromatic π-electron systems. In turn, the pronounced conformational rigidity of the centrohexacyclic framework of 1 enables the unequivocal structural identification of the isomeric hexanitrocentrohexaindanes 14-17 by 1H NMR spectroscopy. © 2016 the Owner Societies.

Neubauer K.,University of Rostock | Huhns M.,University of Rostock | Hausmann T.,University of Rostock | Klemke F.,Humboldt University of Berlin | And 6 more authors.
Journal of Biotechnology | Year: 2012

A chimeric cyanophycin synthetase gene composed of the cphA Te coding region from the cyanobacterium Thermosynechococcus elongatus BP-1, the constitutive 35S promoter and the plastid targeting sequence of the integral photosystem II protein PsbY was transferred to the tobacco variety Petit Havanna SRI and the commercial potato starch production variety Albatros. The resulting constitutive expression of cyanophycin synthetase leads to polymer contents in potato leaf chloroplasts of up to 35mg/g dry weight and in tuber amyloplasts of up to 9mg/g dry weight. Both transgenic tobacco and potato were used for the development of isolation methods applicable for large-scale extraction of the polymer. Two different procedures were developed which yielded polymer samples of 80 and 90% purity, respectively. © 2011 Elsevier B.V.

Lier F.,CITEC | Siepmann F.,CITEC | Paul-Stueve T.,CITEC | Wrede S.,Universitatsstrasse 25 | And 2 more authors.
ACM International Conference Proceeding Series | Year: 2012

Researchers and other knowledge workers frequently produce and use diverse research artefacts such as papers, data sets, experiment specifications, software, etc. In this, they are often faced with unclear relationships (e.g., which version of a software was in use for a particular paper), creating unnecessary work and potentially errors. Semantic web technologies can provide metadata as well as explicit, specific links between the artefacts. However, data acquisition and perceived utility are potential stumbling blocks for adoption. Therefore, we propose a system which is focused on integrating and augmenting existing data (thus protecting the existing investment), and examine it using an interactionoriented perspective, on users without semantic web experience. Specifically, we first study requirements of the target group and then present an exploratory study of managing research artefacts related to software-centric projects. The results confirm that diverse data sources are in common use, that re-using existing repositories is perceived as efficient (e.g., more convenient, shorter cycle time), and that the experimented aggregates are perceived as functionally relevant. Furthermore, the integration of quality assurance mechanisms, such as continuous integration, is perceived as beneficial, despite some added effort. Copyright 2012 ACM.

Lier F.,CITEC | Schulz S.,CITEC | Lutkebohle I.,Universitatsstrasse 25
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2012

Simulated environments often provide the first, and are usually the most frequent, test environment for robotic systems, primarily due to their cost and safety advantages. Unfortunately, changing aspects of both, the simulation and the real robot, as well as actuator control algorithms are often not taken into account when relying on simulation results. In this paper we present a continuous integration approach to verify simulated robot models in an integrated and frequent manner, comprising a simulated and a real robot for comparison. The central aspect of our concept is to iteratively assess the fidelity of simulated robot models. In an exemplary case study we distilled a first set of requirements and metrics, which can be used by developers to verify their algorithms and to automatically detect further system changes. © 2012 Springer-Verlag Berlin Heidelberg.

Gorzolka K.,Universitatsstrasse 25 | Bednarz H.,Universitatsstrasse 25 | Niehaus K.,Universitatsstrasse 25
Planta | Year: 2014

Mass spectrometry imaging was applied on germinated barley for the detailed localization of metabolites in longitudinal and transversal seed sections. Among others, 20 m/z signals occurred in three regular peak clusters with specific, distinct localizations in embryo tissues. High resolution FT-ICR MS, MALDI-TOF MS/MS, and UHPLC-ESI MS/MS served for the identification and structural characterization of these compounds. Only five metabolites were published in their structures, namely the antifungal compounds hordatine A and B in non-glycosylated and glycosylated forms. All other non-identified cluster compounds were of hordatine-like structure and differed by systematic O-methylations, hydroxylations, and glycosylations. These differences in molecular structures correlated to distinct localization patterns within the embryo and might serve for the regulation of antifungal properties. Based on the structural investigations by mass spectrometry, an array of different hordatines that comprises the five published hordatines, 15 novel hordatine derivates and their six precursors could be localized in the embryo of germinated barley. Implications for the biosynthetic pathway and transport processes are discussed. © 2014 Springer-Verlag Berlin Heidelberg.

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