Universitatea Of Medicina Si Farmacie Craiova

Craiova, Romania

Universitatea Of Medicina Si Farmacie Craiova

Craiova, Romania

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Boujedidi H.,French Institute of Health and Medical Research | Boujedidi H.,University Paris - Sud | Bouchet-Delbos L.,French Institute of Health and Medical Research | Bouchet-Delbos L.,University Paris - Sud | And 16 more authors.
Alcoholism: Clinical and Experimental Research | Year: 2012

Background: Quantification of gene expression using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) requires normalization to an endogenous reference gene termed housekeeping gene (HKG). Many of the commonly used HKGs are regulated and vary under experimental conditions and disease stages. Alcoholic liver disease (ALD) is associated with several different liver histological lesions that may modulate HKG expression. We investigated the variability of commonly used HGKs (18S, β-actin, glyceraldehyde-3-phosphate [GAPDH], and arginine/serine-rich splicing factor [SFRS4]) in the liver of patients with ALD. Methods: Fifty consecutive patients at different stages of ALD underwent liver biopsy. The stability of HKG was assessed according to liver histological lesions. Results: β-actin had the highest coefficient of dispersion (COD) (23.9). β-actin tended to decrease with steatosis and to increase with alcoholic hepatitis; β-actin also increased in patients with both alcoholic hepatitis and cirrhosis. GAPDH and SFRS4 COD were 2.8 and 2.1, respectively. GAPDH was decreased with steatosis and increased with alcoholic hepatitis and fibrosis. 18S had the lowest COD (1.4). Both 18S and SFRS4 levels were not significantly modified with respect to all alcohol-induced liver histological lesions. Conclusions: In patients with ALD, the most constantly expressed HKGs are 18S and SFRS4. These genes are appropriate reference genes for normalization of RT-qPCR in the liver of patients with ALD. The use of other HKGs such as β-actin or GAPDH would lead to misinterpretation of the results. © 2011 by the Research Society on Alcoholism.


Patouraux S.,French Institute of Health and Medical Research | Patouraux S.,University of Nice Sophia Antipolis | Bonnafous S.,French Institute of Health and Medical Research | Bonnafous S.,University of Nice Sophia Antipolis | And 26 more authors.
PLoS ONE | Year: 2012

Background: Osteopontin (OPN) plays an important role in the progression of chronic liver diseases. We aimed to quantify the liver, adipose tissue and serum levels of OPN in heavy alcohol drinkers and to compare them with the histological severity of hepatic inflammation and fibrosis. Methodology/Principal Findings: OPN was evaluated in the serum of a retrospective and prospective group of 109 and 95 heavy alcohol drinkers, respectively, in the liver of 34 patients from the retrospective group, and in the liver and adipose tissue from an additional group of 38 heavy alcohol drinkers. Serum levels of OPN increased slightly with hepatic inflammation and progressively with the severity of hepatic fibrosis. Hepatic OPN expression correlated with hepatic inflammation, fibrosis, TGFβ expression, neutrophils accumulation and with the serum OPN level. Interestingly, adipose tissue OPN expression also correlated with hepatic fibrosis even after 7 days of alcohol abstinence. The elevated serum OPN level was an independent risk factor in estimating significant (F≥2) fibrosis in a model combining alkaline phosphatase, albumin, hemoglobin, OPN and FibroMeter® levels. OPN had an area under the receiving operator curve that estimated significant fibrosis of 0.89 and 0.88 in the retrospective and prospective groups, respectively. OPN, Hyaluronate (AUROC: 0.88), total Cytokeratin 18 (AUROC: 0.83) and FibroMeter® (AUROC: 0.90) estimated significance to the same extent in the retrospective group. Finally, the serum OPN levels also correlated with hepatic fibrosis and estimated significant (F≥2) fibrosis in 86 patients with chronic hepatitis C, which suggested that its elevated level could be a general response to chronic liver injury. Conclusion/Significance: OPN increased in the liver, adipose tissue and serum with liver fibrosis in alcoholic patients. Further, OPN is a new relevant biomarker for significant liver fibrosis. OPN could thus be an important actor in the pathogenesis of this chronic liver disease. © 2012 Patouraux et al.


Jalba C.S.,Spitalul Clinic De Urgenta Sfantul Pantelimon Bucharest | Jalba B.A.,Spitalul Mai De Urgenta Profesor Dr. Dimitrie Gerota | Vladoi A.-D.,Academy of Economic Studies Bucharest | Zlatian O.,Universitatea Of Medicina Si Farmacie Craiova | And 4 more authors.
Annals of the Romanian Society for Cell Biology | Year: 2010

To study the expression status and clinical relevance of vascular endothelial growth factor- A (VEGF-A) in colorectal cancer (CRC) tissues. VEGF-A expression was investigated by immunohistochemistry in 89 cases with CRC. Some demographic and histopathologic variables were compared with VEGF-A expression to determine the prognostic significance in CRC. VEGF-A (-) was found in 24 cases; (+), (++) and (+++) stainings were detected in 24, 35 and 6 cases, respectively. VEGF-A (-) was found in 20 of 58 cases with left colon cancer, while only 4 of 31 cases with right colon cancer were VEGF-A (-)(P = 0.024). There was a trend for lower tumor grade and lesser serosal invasion in cases with VEGF-A (-) samples (P = 0.07 and P = 0.079, respectively). Although the correlation was not statistically significant, there was a trend for lower death rate in cases with VEGF-A (-) tumor (P = 0.087). The longest survival was found in cases with VEGF-A (-) tumor and the shortest survival was found in cases with VEGF-A (+++) tumor. Median survival for patients with VEGF-A (-), (+), (++) and (+++) tumors was 59, 47, 35 and 11 months, respectively (P = 0.02). The Cox proportional hazards model identified stage IV disease and VEGF-A (+++) tumor as having the most important influences upon overall survival (odds ratio: 5.1, 95% confidence interval: 2.0-13.0 and odds ratio: 3.6, 95% confidence interval: 1.0-12.7, respectively), followed by serosal invasion (odds ratio: 2.4, 95% confidence interval: 1.0-5.9). This study shows that VEGF-A is a poor prognostic factor in cases with CRC but the relatively small size of the study group precluded the correlation with all the known prognostic indicator.


Jalba B.A.,Spitalul Mai De Urgenta Profesor Dr. Dimitrie Gerota | Jalba C.S.,Spitalul Clinic De Urgenta Sfantul Pantelimon Bucharest | Vladoi A.-D.,Academy of Economic Studies Bucharest | Gherghina F.,Universitatea Of Medicina Si Farmacie Craiova | And 2 more authors.
Annals of the Romanian Society for Cell Biology | Year: 2010

Osteoarthritis is characterized by a progressive degradation of cartilage structure and function. This study tests the hypothesis that disease severity is characterized by alterations in expression of cartilage-specific genes for aggrecan and collagen type II. Cartilage, discarded from six subjects undergoing knee replacement, was subdivided into homogeneous portions by the surgeon according to the Outerbridge classification. For four subjects, it was possible to separate the tissue into two or three fractions with different disease severity. Portions of each sample were prepared either for histological analysis and ranking according to the Mankin system or for RNA extraction. Quantitative, competitive RT-PCR assays were used for measurement of mRNA for aggrecan, collagen type II, and glyceraldehyde-3- phosphate dehydrogenase. Clinical grading was correlated with histological score (Spearman r=0.60, p=0.043). There was a striking decrease in expression of aggrecan and collagen II that was correlated with increase in the grade in regions of cartilage within an individual subject. In the series of 12 samples, there was an inverse correlation between aggrecan expression and osteoarthritis grade (Spearman r= -0.59, p=0.042). In conclusion, there was an inverse relationship between regional disease severity in osteoarthritis and expression of aggrecan. Use of quantitative, competitive RT-PCR is practical for assessment of chondrocyte gene signatures.


Ene R.-D.,Polytechnic University of Timişoara | Szabo M.A.,Polytechnic University of Timişoara | Danoiu S.,Universitatea Of Medicina Si Farmacie Craiova
Materiale Plastice | Year: 2015

The viscous flow over a shrinking permeable sheet with partial slip is investigated. The flow is governed by a third-order nonlinear differential equation and heat transfer by a second-order differential equation. The equations of motion are solved analitically by Optimal Homotopy Perturbation Method (OHPM). This procedure is highly efficient and it controls the convergence of the approximate solutions. A few examples are presented, showing the exceptionally good agreement between the analytical and numerical solutions. OHPM is very efficient in practice, ensuring a very rapid convergence after only one iteration.


Jalba C.S.,Spitalul Clinic De Urgenta Sfantul Pantelimon | Jalba B.A.,Spitalul Mai De Urgenta Profesor Dr. Dimitrie Gerota | Casabalian D.,Spitalul Mai De Urgenta Profesor Dr. Dimitrie Gerota | Marculescu M.,Spitalul Mai De Urgenta Profesor Dr. Dimitrie Gerota | And 2 more authors.
Annals of the Romanian Society for Cell Biology | Year: 2010

Purpose: Vascular endothelial growth factor (VEGF) or its family may be considered to play an important role in lymphangiogenesis and lymphatic tumor spread, thereby affecting prognosis of colorectal cancer. Accordingly, the present study analyzed VEGF gene polymorphisms and their effect on the prognosis for patients with colorectal cancer. Experimental Design: Four hundred and forty-five consecutive patients with surgically treated colorectal adenocarcinoma were enrolled in the present study. The genomic DNA was extracted from fresh colorectal tissue and three VEGF (-2578C>A, -634G>C, and + 936C>T) gene polymorphisms were determined using a PCR/denaturing high-performance liquid chromatography assay. Results: Multivariate survival analysis showed that the survival for the patients with the - 634G/C genotype [overall survival (OS): hazard ratio (HR), 0.158; P < 0.001] or C/C genotype (OS: HR, 0.188; P < 0.001) were better than for the patients with the -634G/G genotype, whereas the +936C/T genotype (OS: HR, 12.809; P < 0.001) or T/T genotype (OS: HR, 37.260; P < 0.001) was associated with a worse survival compared with the +936 C/C genotype. In haplotype analysis, the -2578A/-634G/+936T haplotype exhibited a significantly worse survival when compared with the wild -2578C/-634G/+936C haplotype (OS: HR, 3.866; P < 0.001). Conclusions: VEGF gene polymorphisms were found to be an independent prognostic marker for patients with colorectal cancer. Accordingly, the analysis of VEGF gene polymorphisms can help identify patient subgroups at high risk of a poor disease outcome.


Didilescu C.,Universitatea Of Medicina Si Farmacie Craiova | Tanasescu M.,Institutul Of Pneumologie Marius Nasta
Pneumologia | Year: 2012

In the actual context of an increased TB endemia (notification rate of 90.5%ooo, meaning 21457 cases in 2010, but with a constant decreasing trend in the last 8 years), we wanted to see what is the share and structure of extra-respiratory tuberculosis in the period 2007-2010. In the interval 2007-2010 have been registered annually between 1252-1267 extra-respiratory TB cases. Extra-respiratory TB have been between 30% and 42,1% from all TB cases registered annually with the extra-pulmonary TB. In the descending order of cases recorded with TB extra respiratory in 2010, the first was extra-thoracic ganglionary TB (244 cases), followed by osteo-articulary (233) and those of meningo- encephalitis and CNS TB (133). Location of TB on the spine remains the most common form of skeletal TB, representing 62.2% (145 cases) of all osteo-articulary locations. The number of registered cases of pericardial effusions TB annually remains steady at 40-50 cases. The number still high of meningo encephalitis TB (severe prognosis, epidemiological severity) involves enhanced accountability measures in TB control of the territory. The collaboration between the pulmonologist and the body specialist constitutes compulsory condition of quality assistance in case of TB extra respiratory sites.


Tanasescu M.,Institutul Of Pneumologie Marius Nasta | Didilescu C.,Universitatea Of Medicina Si Farmacie Craiova | Marica C.,Carol Davila University of Medicine and Pharmacy
Pneumologia | Year: 2013

Tuberculosis is still one of the diseases with a major medical and social impact, and in terms of early diagnosis (which would imply a fair treatment and established at the time), difficulties related to the delay bacilli isolation in culture, decreased susceptibility testing methods to antituberculosis drugs, lack of methods for differentiation of M. Tuberculosis complex germs of non-TB Mycobacteria, may have important clinical implications. Traditional testing of anti-TB drug susceptibility on solid Löwenstein-Jensen medium (gold standard) or liquid media can only be performed using grown samples. Determining the time it takes up to 42 days on solid media and 12 days for liquid media. For MDR/XDR TB cases it is absolutely essential to reduce the detection time. In these cases rapid diagnostic methods prove their usefulness. Automatic testing in liquid medium, molecular hybridization methods are currently recommended by the current WHO guidelines. Rapid diagnosis of MDR-TB is extremely useful for the early establishment of an effective treatment tailored more accurately on the spectrum of sensitivity of the resistant strain (thus reducing the risk of developing additional resistance to other drugs) and control the spread of these strains. Genetic diagnostic methods, approved and recommended by the WHO, can reduce the time of diagnosis of TB case and, importantly, the case of MDR-TB. They do not replace the current standard diagnostic methods and resistance profile, but complete them in selected cases.


Prejbeanu I.,Universitatea Of Medicina Si Farmacie Craiova
Revista medico-chirurgicalǎ̌ a Societǎ̌ţii de Medici ş̧i Naturaliş̧ti din Iaş̧i | Year: 2010

The purpose of the study was to evaluate the amplitude of tobacco and alcohol consumption among two populations living in urban, respectively rural areas in the South-Western part of the country. The presence of the two risky behaviours during the pregnancy was also analysed. A number of 1,200 people, men and women, aged 18-75 years, with different educational levels, living in urban and rural communities were asked to answer a questionnaire with 111 items regarding their knowledge and attitudes about the sexual-reproductive health; 11 items referred to tobacco smoking and alcohol drinking. About half of the subjects have never smoked. The mean age of starting smoking was 16.97 years. A number of 295 subjects (24.6%) were daily active smokers, men more frequently than women (p < 0.001). A percentage of 77.3% of the subjects used to drink alcohol, 16.4% of them daily. The daily alcohol consumption was more frequent among men, low educated people and rural inhabitants comparing to women, respectively high educated or urban inhabitants (p < 0.001); 11.2% of the respondents got frequently drunk. During the last pregnancy in the couple, women and high educated people stopped smoking and drinking more frequently then men, respectively low educated people. The above findings indicate the need to start in school effective health promotion campaigns to reduce tobacco and alcohol consumption and the need for support of population-based educational interventions aimed at smoking cessation in both parents.


PubMed | Universitatea Of Medicina Si Farmacie Craiova
Type: Comparative Study | Journal: Pneumologia (Bucharest, Romania) | Year: 2012

In the actual context of an increased TB endemia (notification rate of 90.5 per thousand, meaning 21457 cases in 2010, but with a constant decreasing trend in the last 8 years), we wanted to see what is the share and structure of extra-respiratory tuberculosis in the period 2007-2010. In the interval 2007-2010 have been registered annually between 1252-1267 extra-respiratory TB cases. Extra-respiratory TB have been between 30% and 42,1% from all TB cases registered annually with the extra-pulmonary TB. In the descending order of cases recorded with TB extra respiratory in 2010, the first was extra-thoracic ganglionary TB (244 cases), followed by osteo-articulary (233) and those of meningo-encephalitis and CNS TB (133). Location of TB on the spine remains the most common form of skeletal TB, representing 62.2% (145 cases) of all osteo-articulary locations. The number of registered cases of pericardial effusions TB annually remains steady at 40-50 cases. The number still high of meningo encephalitis TB (severe prognosis, epidemiological severity) involves enhanced accountability measures in TB control of the territory. The collaboration between the pulmonologist and the body specialist constitutes compulsory condition of quality assistance in case of TB extra respiratory sites.

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