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Somaggio L.R.,Sociedade Educacao e Caridade Colegio Purissimo Coracao de Maria | Vicente A.A.,Sociedade Educacao e Caridade Colegio Purissimo Coracao de Maria | Marin R.J.,Sociedade Educacao e Caridade Colegio Purissimo Coracao de Maria | De Carvalho R.,Laboratorio Of Ciencias | And 3 more authors.
Revista em Agronegocio e Meio Ambiente | Year: 2012

This paper aimed to assess the seed germination and early growth of sunflower (Helianthus annuus L.) seedlings subjected to pre-soaking in gibberellin. A completely randomized design was used with four replications of 25 seeds which were soaked in water for 24 hours (control) and for the same period in solutions of gibberellic acid (GA3) at concentrations of 5, 10 and 20 mg. L 1. Seeds were placed on two sheets of filter paper previously moistened with 10 mL of distilled water in petri dishes and subjected to an average temperature of 28 ± 2° C under white light from fluorescent lamps at 32.85 μmol.m2.s-1 at the seed level until germination. The variables Germinability (G%), Index of Germination Speed (IVG), total length of seedlings (CP), shoot length (CA), longest root length (RL), Fresh Matter (FM) and Dry Matter (DM). The results indicated that pre-soaking the sunflower seeds at concentrations of 5 mg l-1 GA3resulted in better values for all variables, except for the longest root length and germination speed index for which the concentration of 10 mg. L-1 GA3 gave better results. Compared to the highest concentrations of GA3 currently used in sunflower cultivation, it is possible to reduce production costs by using lower concentrations of plant growth regulator as found in this work for the species.


Massaro R.,Centro Universitario Herminio Ometto | Cordeiro G.M.,Centro Universitario Herminio Ometto | De Souza-Leal T.,Centro Universitario Herminio Ometto | Pedroso-de-Moraes C.,Centro Universitario Herminio Ometto | Pedroso-de-Moraes C.,Universitario Herminio Ometto
Revista em Agronegocio e Meio Ambiente | Year: 2012

The in vitro seeding is an indispensable tool to propagate the main commercial species of orchids, such as Epidendrum secundum, a species of great importance in obtaining hybrids. The present work aimed to study aspects of the in vitro development of this species by assessing the effect of MS medium with half of its concentration of macronutrients and two media based on fertilizers, Hyponex and Kristalon Laranja. The culture media were autoclaved at 121 ° C and 1 atm pressure for 20 minutes. The seeds were disinfected by being stirred in Eppendorf ® tubes, containing 5% sodium hypochlorite, for five minutes. Subsequently, they were washed and inoculated in flasks with 1 ml of distilled water. Four flasks were seeded per treatment with 1 g of seeds each. After 180 days of cultivation, the most efficient medium for the development of in vitro plantlets was inferred to be the MS medium with half of its concentration of macronutrients, which had the highest means for all the biometric variables assessed. Therefore, it can be commercially used by small producers since it presents a lower production cost.


Caovila L.E.,Universitario Herminio Ometto | Gianini P.F.,Universitario Herminio Ometto Of Araras | De Moraes C.P.,Universitario Herminio Ometto
Revista em Agronegocio e Meio Ambiente | Year: 2016

The effect of different concentrations of sucrose and pH indexes in the in vitro growth of Oncidium fexuosum is evaluated. Plants were cultivated in a MS/2 culture medium to test the effects of sucrose (0, 15, 30, 45, 60 g·L-1) and pH (5.0, 5.8, 6.6) on their development with respect to the variables: height of aerial part (HAP), number of roots (NR), length of the greatest root (LGR), number of leaves (NL) and fresh mass (FM). Regardless of pH, concentrations 15 and 30 g·L-1 were the most efficient for the speciesś in vitro culture since they enhanced increase in root length, height of plant and fresh mass. Further, pH was significant only when combined to 15 g·L-1 of sucrose for the length of the greatest root and fresh mass.


Stencel M.,Universitario Herminio Ometto Of Araras Uniararas | Bertin R.L.,Universitario Herminio Ometto Of Araras Uniararas | Souza-Leal T.,Claro | Pedroso-de-Moraes C.,Universitario Herminio Ometto
Revista em Agronegocio e Meio Ambiente | Year: 2016

Phenotypic plasticity is the genotypic capacity in producing different phenotypes as a response to different environmental conditions. The invading orchid Oeceoclades maculata has high adaptative capacity related to the colonization of different habitats, particularly, anthropic ones. Current paper provides morphobiometric analyses of the species to report plastic-phenotypic-vegetative adaptations in two luminic environments in the Arboreto de Essências Nativas, in Araras SP Brazil. Fifty specimens of Oeceoclades maculata, featuring physiological maturity, were collected from the edge and the interior of the forest fragment. The following biometric data were taken: mean number of roots and pseudobulbs, leaves, mean length of roots and pseudobulbs, leaves, mean diameter of leaves and pseudobulbs, mean length and thickness of leaves, fresh mass of roots and pseudobulbs and leaves, dry mass of roots and pseudobulbs and leaves. Phenotypic plasticity index was calculated for all morphobiometric variables. Morphobiometric variables revealed plastic-phenotypic-vegetative adaptations in Oeceoclades maculata specimens in different environments of the Arboreto de Essências Nativas. Adaptations are mainly co-related to luminic conditions on the fragmentś edge and interior.


Verdolin-Benedito P.,Elektro Distribuidora de Energia S.A. | Curiel A.C.,Universitario Herminio Ometto | Pedroso-De-Moraes C.,Universitario Herminio Ometto
Revista em Agronegocio e Meio Ambiente | Year: 2012

Percentage, speed, mean speed, mean time of germination and growth of Mimosa caesalpiniaefolia by treatment of seeds with gibberellic acid (GA 3) are provided. Seeds underwent pre-soaked for 0.5, 1, 2 and 3 hours in water and concentrations of 5, 10 and 20 mg.L-1 GA3. Pre-Soaked seeds were distributed in four petri dishes with 25 seeds each. They were previously wrapped in two sheets of filter paper wetted with 10 mL of distilled water and left at a BOD climatic chamber at 25°C ± 2 and under a fluorescent lamp white light at 32,85 μmol.m-2.s -1 at seed level until germination. Results from germination tests and tests with regard to plant development such as length of roots, length of aerial parts, total plant length, fresh and dry matter were submitted to analysis of variance and means were compared by Tukey's test 5%. Treatments with different concentration of GA3 did not affect significantly the germination processes. However, pre-soaked for 2 hours at 10 and 20 mg.L -1 of GA3 increased the plants' fresh matter. Preliquid absorption for 3 hours at any GA3 concentration positively affected total plant and root length when compared to the other treatments.

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