Lo Piccolo L.,Universita Degli Studi Of Palermo |
Bonaccorso R.,Universita Degli Studi Of Palermo |
Onorati M.C.,Universita Degli Studi Of Palermo
International Journal of Molecular Sciences | Year: 2015
The identification and study of protein’s function in several model organisms is carried out using both nuclear and cytoplasmic extracts. For a long time, Drosophila’s embryos have represented the main source for protein extractions, although in the last year, the importance of collecting proteins extracts also from larval tissues has also been understood. Here we report a very simple protocol, improved by a previously developed method, to produce in a single extraction both highly stable nuclear and cytoplasmic protein extracts from a small quantity of whole Drosophila’s larvae or tissues, suitable for biochemical analyses like co-immunoprecipitation. © 2015 by the authors; licensee MDPI, Basel, Switzerland.
Pojero F.,Universita Degli Studi Of Palermo |
Pojero F.,Uosd Laboratorio Specialistico Oncologia |
Casuccio A.,Universita Degli Studi Of Palermo |
Giambanco C.,Uosd Laboratorio Specialistico Oncologia |
And 6 more authors.
Leukemia Research | Year: 2016
Background/Aims Multiple myeloma (MM) is caused by proliferation of clonal plasma cells (cPCs) in bone marrow (BM), associated with numerical and functional defects in immune subsets. An impairment of B cell compartment is involved in onset/progression of the disease. Methods: By flow cytometry, we studied distribution of naïve/transitional (IgD+CD27−), memory unswitched (IgD+CD27+), memory switched (IgD−CD27+) and double negative (DN) (IgD−CD27−) B lymphocytes in BM of control subjects, and responding and relapsing patients. Results: We observed an increased percentage of IgD+CD27+ B cells in healthy controls vs responding patients (p < 0.05). Treated non complete responders exhibited an expanded DN compartment vs stringent complete responders (p = 0.011); in turn IgD+CD27− subpopulation was larger in stringent complete responders vs other responding patients (p = 0.006). None of the studied B cell subsets showed clonal restriction. Correlation analysis revealed negative correlations between naïve/transitional and DN B cells in all groups, except in newly diagnosed subjects. Conclusions: This may be considered a feasible start point to explore the importance of B cells in the immunosuppressive MM BM microenvironment, correlating these findings with immunosenescence and therapy related increased risk of infection. Moreover, we propose a possible role of naïve/transitional and DN B cells as predictive markers in treated patients. © 2016 Elsevier Ltd
Gallea R.,Universita Degli Studi Of Palermo |
Ardizzone E.,Universita Degli Studi Of Palermo |
Pirrone R.,Universita Degli Studi Of Palermo
Communications in Computer and Information Science | Year: 2015
Advance in image technology and proliferation of acquisition devices like smartphones, digital cameras, etc., made the display of digital images ubiquitous. Many displays exist in the market, spanning within a large variety of resolutions and shapes. Thus, displaying content optimizing the available number of pixels has become a very important issue in the multimedia community, and the image retargeting problem is being widely faced. In this work, we propose an image retargeting framework based on monte-carlo sampling. We operate the non-homogeneous resizing as the composition of several simple atomic resizing functions. The shape of such atomic operator can be chosen within a set of tested functions or the user could design additional ones. Using independent atomic operators allows parallelizing the retargeting procedure. Additionally, since the algorithm does not require any optimization, it could be executed in real-time, which is a key aspect for on-line visualization of multimedia content. © Springer International Publishing Switzerland 2015.
Galioto N.,Universita Degli Studi Of Palermo |
Lo Bue F.,Universita Degli Studi Of Palermo |
Mistretta L.,Universita Degli Studi Of Palermo |
Giaconia C.G.,Universita Degli Studi Of Palermo
Lecture Notes in Electrical Engineering | Year: 2016
A new electrical current measurement system is presented. It features the ability to dynamically and automatically change its measurement range to the sensed current amplitude without user action. It also exhibits galvanic isolation and near zero insertion loss characteristics. © Springer International Publishing Switzerland 2016.
Ala G.,Universita Degli Studi Of Palermo |
Francomano E.,Universita Degli Studi Of Palermo
Journal of Scientific Computing | Year: 2014
Numerical solution of partial differential equations governing time domain simulations in computational electromagnetics, is usually based on grid methods in space and on explicit schemes in time. A predefined grid in the problem domain and a stability step size restriction need. Recently, the authors have reformulated the meshless framework based on smoothed particle hydrodynamics, in order to be applied for time domain electromagnetic simulation. Despite the good spatial properties, the numerical explicit time integration introduces, also in a meshless context, a severe constraint. In this paper, at first, the stability condition is addressed in a general way by allowing the time step increment get away from the minimum points spacing. Then, an alternating direction implicit leapfrog scheme for time evolution is proposed. The unconditional stability of the method is analytically provided and numerically validated. The stability of the method has been proved by avoiding the algebra developments related to the usually adopted von Neumann analysis. Three case studies are investigated by achieving a satisfactory agreement by comparing both numerical and analytical results. © 2014, Springer Science+Business Media New York.
Pumo D.,Universita` Degli Studi Of Palermo |
Viola F.,Universita` Degli Studi Of Palermo |
La Loggia G.,Universita` Degli Studi Of Palermo |
Noto L.V.,Universita` Degli Studi Of Palermo
Journal of Hydrology | Year: 2014
Flow duration curve (FDC) represents a comprehensive signature of temporal runoff variability often used to synthesize catchment rainfall-runoff responses. A new model, the ModABa (MODel for Annual flow duration curves assessment in ephemeral small BAsins), is here introduced. It can be thought as a wide mosaic whose tesserae are frameworks, models or conceptual schemes separately developed in different studies and harmoniously interconnected with the final aim of reproducing the annual FDC in intermittent small catchments. Two separated seasons within the hydrological year are distinguished: a dry season, characterized by absence of streamflow, and a non-zero season. Streamflow is disaggregated into a subsurface component and a surface component that, in turn, is considered formed by two different contributions: impervious runoff and surface runoff from permeable areas induced by heavy rains. The FDCs of the two streamflow components are first separately and differently computed, and then combined to obtain the non-zero FDC. This last, together with the estimated probability of null streamflow, allows the annual FDC assessment through the theory of total probability. The ModABa is here tested on a small Italian catchment and the results show how the model, once calibrated, is able to accurately reproduce the empirical FDC for the analyzed case, starting from easily derivable parameters and commonly available climatic data. In this sense, the model reveals itself as a valid tool, potentially suitable for predictions at ungauged basins in a regionalization framework. © 2014 Elsevier B.V.