United Institute of Pharmacy

Allahābād, India

United Institute of Pharmacy

Allahābād, India
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Rohini A.,United Institute of Pharmacy | Agrawal N.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Sara U.V.S.,Raj Kumar Goel Institute of Technology
International Journal of Current Pharmaceutical Review and Research | Year: 2013

The present study has been designed to evaluate the protective effect of Zingiber Officinale in experimental cardiac hypertrophy in rats. The treatment with ethanolic extract of Zingiber Officinale (EZO) (200 mg/kg and 400 mg/kg) was started three days before surgery and it was continued for 4 weeks after surgery. The development of left ventricular (LV) hypertrophy was assessed by measuring ratio of LV weight to body weight (LVW/BW), LV wall thickness (LVWT), LV protein content, LV collagen content and LV RNA concentration. Further mean arterial blood pressure (MABP) was recorded. The PAAC significantly increased the ratio of LV weight to body weight, LV wall thickness, LV protein content, LV collagen content and LV RNA concentration. Further PAAC significantly increased MABP in dose dependent manner. The EZO (400mg/kg) markedly attenuated PAAC induced increase in LV hypertrophy and MABP. These results implicate for the first time the role of Zingiber Officinale in PAAC induced pathological cardiac hypertrophy.


Mishra S.B.,United Institute of Pharmacy | Mukerjee A.,United Institute of Pharmacy | Vijayakumar M.,National Botanical Research Institute
Pharmacologyonline | Year: 2010

The influence of leave extract of Jasminum grandiflorum was studied for its wound healing activity at a dose of 250 mg/kg body weight, using excision and dead space wound models in rats. The animals were divided into three groups in excision wound model, the controls (n=6) were treated with 0.25% CM cellulose, reference standard (n=6) were treated with sulfathiazole ointment and the experimental (n=6) were treated with extract of J. grandiflorum leave till complete epithelialization. The animals in dead space wound models were divided into two groups, controls were given plain drinking water and the experimental animals were administered with extract orally for 10 days. The extract treated wounds were found to epithelize faster as compared to controls. Extract treated rats exhibited 65% reduction in the wound area when compared to controls (54%). The wet and dry granulation tissue weight, and hydroxyproline content in a dead space wound model increased significantly (P<0.001) when compared to controls. Histological studies of the tissue obtained on day 10 from the extracttreated group showed increased well organized bands of collagen, more fibroblasts and few inflammatory cells when compared to controls which showed inflammatory cells, scanty collagen fibres and fibroblasts. The demonstration of increased rate of wound contraction together with the biochemical and histological findings suggest the use of J. grandiflorum leave extract in the management of wound healing.


Mishra S.B.,United Institute of Pharmacy | Pandey H.,Allahabad University | Pandey A.C.,Allahabad University
Advances in Natural Sciences: Nanoscience and Nanotechnology | Year: 2013

Phyllanthus amarus (P. amarus) is commonly used for traditional Indian medicine and as dietary adjuncts for the treatment of numerous physiological disorders including hepatic disorders. Due to the poor water solubility of its major constituents such as lignans and flavonoids, its absorption upon oral administration could be limited. The present study was designed to evaluate and compare the hepatoprotective effects of the ethanolic extract of P. amarus (PAE) and its nanoparticles (PAN) on paracetamol induced acute liver toxicity in Sprague-Dawley rats. An oral dose of PAE at 125 and 250 mg kg-1 and PAN at 25 and 50 mg kg-1 showed a significant hepatoprotective effect relatively to the same extent (P < 0.001) by reducing levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and bile salts. These biochemical assessments were supported by rat hepatic biopsy examinations. Moreover, the results also indicated that the hepatoprotective effect of 50 mg kg-1 PAN was effectively better than 125 mg kg -1 PAE (P < 0.001), and an oral dose of PAN that is five times less than PAE could exhibit similar levels of outcomes. In conclusion, we suggest that the nanoparticles system can be applied to overcome other poorly water soluble herbal medicines and furthermore to decrease the treatment dosage. © 2013 Vietnam Academy of Science & Technology.


Ghosh S.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Verma A.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Mukerjeeb A.,United Institute of Pharmacy | Mandal M.K.,United Institute of Pharmacy
Der Pharmacia Lettre | Year: 2014

The present investigation is synthesis of some novel 1, 2, 4-triazolo[1, 3, 4]thiadiazoles derivatives with an aim of discovering potent antimicrobial agents that can be helpful in overcoming antibiotic resistance. A new series of 1, 2, 4-triazolo[3, 4-b][1, 3, 4]thiadiazoles containing substituted phenylquinoline moiety (4a-4t) were synthesized in multiple steps. These derivatives were obtained in good yield by condensation of (R)-4-amino-5-sulfanyl-4, 5- dihydro-1, 2, 4-triazol-4-phenylquinolin-2-one (3a-3t) with aromatic carboxylic acid(benzoic acid) in the presence of phosphorus oxychloride. Then structures of synthesized compounds (4a-4t) were established by their spectral analysis. These newly synthesized compounds (4a-4t) were screened for their invitro antimicrobial activity using Broth Micro Dilution technique. Bacterial strains of Escherichia coli (ATCC 8739), Pseudomonas aeruginosa(MCCB 0035), Staphylococcus aureus (ATCC 29213), and the fungal strains of Aspergillusfumigatus(NCIM 2081), Aspergillusniger(NCIM 2191), Candida albicans(NCIM 2087) were used.Compound 4t showed more potent activity against gram negative bacterial strain Escherichia coli than the standard drug Ampicillin anhydride and compound 4f, 4i, 4swere found to be more potent against fungal strain Aspergillusfumigatuswhen compared with standard drug Fluconazole.


Ghosh S.,United Institute of Pharmacy | Mandal M.K.,United Institute of Pharmacy
Indian Drugs | Year: 2011

A series of 1, 2, 4-triazolophenyl quinoline derivatives were synthesized as potent fungicidal agents. The newly synthesized compounds were then characterized by spectral and elemental analysis. The synthesized compounds were screened for their antifungal activity against C. albicans and A. niger. All compounds carrying 1, 2, 4-triazole moiety showed significant biological activity.


Srivastava R.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Mukerjee A.,United Institute of Pharmacy | Verma A.,Sam Higginbottom Institute of Agriculture, Technology and Sciences
Pharmacognosy Journal | Year: 2015

Introduction: Wrightia tinctoria R.Br. (Family: Apocynaceae) commonly called "Indrajau" is well known in Indian traditional system for its traditional uses. Materials and Methods: The present investigation was carried out to determine the possible bioactive components of plant seed ethanolic extract, pet ether fraction using GC-MS analysis. 22 components were identified from pet ether fraction obtained from elution of ethanolic extract packed in silica column. Results: The prevailing compounds from fraction F6to F9 were [1,1'-Bicyclopropyl]-2-octanoic acid, 2'-hexyl-, methyl ester (21.39%) , Trilinolein (7.74%), 2-Myristynoyl pantetheine (18.07%), 9-Octadecen-12-ynoic acid, methyl ester (4.46%), 1Hexadecanol,2-methyl (3.77%), Cyclopropane tetradecanoic acid, 2-octyl-, methyl ester (2.36%), 1b, 4a-Epoxy-2H-cyclopenta [3,4] cyclopropa [8,9]cycloundec [1,2-b]oxiren-5 (6H)-one, 7-(acetyloxy) decahydro-2,9,10- Trihydroxy-3,6,8,8,10a-pentamethyl (38.91%), Geranyl isovalerate (23.58%), cis-13-Octadecenoic acid (5.91%), Quassin (3.82%), cis-10-Heptadecenoic acid (3.08%), 9,12,15-Octadecatrienoic acid 2-phenyl-1, 3-dioxan-5-yl ester (31.50%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-2,3-dihydroxypropyl ester (14.35%), Cyclopropanebutanoic acid, 2-[ [2-[ [2- [(2-pentylcyclopropyl) methyl] cyclopropyl] methyl] cyclopropyl] methyl]-, methyl ester (10.13%), 6,9,12,15-Docosatetraenoic acid, methyl ester (3.39%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, trans-( 2.73%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, cis-(4.34%), Ursodeoxycholic acid (7.14%), Bufa-20,22-dienolide, 3-(acetyloxy)-14,15-epoxy-16-hydroxy-, (3á,5á,15á,16á)-(4.75%), 5H-Cyclopropa [3,4] benz [1,2-e]azulen-5-one, 9a (acetyloxy)-1,1a,1b,4,4a,7a,7b,8,9,9a-de cahydro-4a,7b,9- Trihydroxy-3-(hydroxymethyl)-1,1,6,8- Tetramethyl-,[1aR-(1aà,1bá,4aá,7aà,7bà,8à,9á,9aà)]-(6.59%), Docosahexaenoic acid, 1,2,3-propanetriyl ester (10.86%), Olean-12-ene-3,15,16,21,22,28-hexol, (3á,15à,16à,21á,22à)-( 4.40%) found as the major components. Conclusion: It could be concluded that, Wrightia tinctoria contains various bioactive compounds. So it is recommended as a plant of phytopharmaceutical importance.


Mishra S.B.,United Institute of Pharmacy | Verma A.,Indigenous | Mukerjee A.,United Institute of Pharmacy | Vijayakumar M.,National Botanical Research Institute
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: The aim of the present study was to evaluate the possible antidiabetic effects of Amaranthus spinosus leaf extract (ASEt) against streptozotocin-nicotinamide induced diabetes & oxidative stress in albino rats. Methods: Experimental diabetes was induced by a single dose of STZ (60 mg/kg) administered by intraperitoneal way after the administration of nicotinamide (120mg/kg). The oxidative stress was measured by reduced glutathione (GSH) content and by enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in liver and kidney. Biochemical observations were further substantiated with histological examination of pancreas, kidney and liver. Results: The increase in blood glucose with the decrease in GSH content and in enzymatic activities were the salient features observed in diabetic rats. Administration of ASEt (250 & 500 mg/kg bw/day, i.p) for 21 days caused a significant reduction in blood glucose in STZ-nicotinamide treated rats when compared with diabetic rats. Furthermore, diabetic rats treated with ASEt leaf extract showed a significant increase in the activities of both enzymatic and non-enzymatic antioxidants when compared to those of diabetic rats. Degenerative changes of pancreatic cells in STZ treated rats were minimized to near normal morphology by administration of ASEt leaf extract as evidenced by histopathological examination. Conclusion: Results clearly indicate that Amaranthus spinosus treatment attenuate hyperglycemia by decreasing oxidative stress and pancreatic cells damage which may be attributed to its antioxidative potential. © 2012 Asian Pacific Tropical Biomedical Magazine.


Mishra S.B.,United Institute of Pharmacy | Verma A.,Indigenous | Mukerjee A.,United Institute of Pharmacy | Vijayakumar M.,National Botanical Research Institute
Asian Pacific Journal of Tropical Medicine | Year: 2011

Objective: To evaluate the antihyperglycemic activity of leaves of Hyptis suaveolens using streptozotocin model. Methods: Hyptis suaveolens extract (HSE) 250 and 500 mg/kg body weight was administered orally to streptozotocin induced diabetes, once daily for 21 days. Results: A significant reduction in blood glucose was observed in diabetic animals treated with HSE at different doses when compared with diabetic rats. Levels of triglyceride, total cholesterol, low density lipoprotein, very low density lipoprotein were decreased while administering HSE at different doses, compared with their control values in diabetic animals. Conclusions: Our results show that HSE possesses significant antihyperglycemic activity which might be attributed to stimulating effects on glucose utilization and antioxidant enzyme. © 2011 Hainan Medical College.


Ahmed D.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Sharma M.,Jamia Hamdard University | Mukerjee A.,United Institute of Pharmacy | Ramteke P.W.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Kumar V.,Sam Higginbottom Institute of Agriculture, Technology and Sciences
BMC Complementary and Alternative Medicine | Year: 2013

Background: The present study was undertaken to evaluate the antihyperglycemic, antihyperlipidemic and hepatoprotective effect of a traditional unani formulation " Qurs Tabasheer" in streptozotocin (STZ) induced diabetic wistar rats. Up till now no study was undertaken to appraise the efficacy of " Qurs Tabasheer" in the diabetic rats. Qurs Tabasheer is a unani formulation restraining preparations from five various herbs namely Tukhme Khurfa (Portulaca oleracea seed), Gule Surkh (Rosa damascena flower), Gulnar (Punica granatum flower), Tabasheer (Bambusa arundinasia dried exudate on node), Tukhme Kahu (Lactuca sativa Linn seed).Methods: Effect of Qurs Tabasheer was assessed in STZ (60 mg/kg, i.p single shot) induced diabetic wistar rats. STZ produced a marked increase in the serum glucose, Total Cholesterol, LDL cholesterol, VLDL Cholesterol, Triglycerides and trim down the HDL level. We have weighed up the effect of Qurs Tabasheer on hepatic activity through estimating levels of various liver enzymes viz. Hexokinase, Glucose-6-Phosphatase and Fructose-1-6-biphosphatase in STZ diabetic wistar rats.Results: In STZ-induced diabetic wistar rats level of Hexokinase, and Glucose-6-Phosphatase was decreased to a significant level while the level of fructose-1-6-biphophatase was augmented. Therapy with Qurs Tabasheer for 28 days to STZ-induced diabetic rats significantly reduces the level of serum glucose, total cholesterol, triglycerides, glucose-6-phosphatase and fructose-1-6-biphosphatase, while magnitude of HDL cholesterol and hexokinase was amplified.Conclusion: Antihyperglycemic, antihyperlipidemic activity of Qurs Tabasheer extract in STZ- induced wistar rats was found to be more effective than standard oral hypoglycemic drug Glimepiride. © 2013 Ahmed et al.; licensee BioMed Central Ltd.


Ghosh S.,United Institute of Pharmacy | Mandal M.K.,United Institute of Pharmacy
Journal of the Indian Chemical Society | Year: 2013

A series of 1,2,4-triazoIophenyIquinoiine derivatives were synthesized by cyclisation method. Synthesized molecules were characterized by spectral and elemental analysis. These newly synthesized molecules were screened for their in vitro antioxidant activity. Some of the synthesized compounds showed potent antioxidant activity.

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