Allahābād, India
Allahābād, India

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Pathogenic microbes have mutated and developed resistance to the latest range of antibiotics, which has kept synthetic chemist hunting for better and least toxic antimicrobial agents. Cyclocondensation of substituted anilines with 3-phenyl-2-propenoic acid yielded Phenyl-(substituted)-quinolin-2-one derivatives (1a-1t). In the next step phenylquinolin-2-one acetic acid derivatives (2a-2t) were prepared by the treatment of (1a-1t) with chloroacetic acid. Further (2a-2t) derivatives were reacted with thiocarbohydrazide to obtain a series of 4-amino-5-sulfanyl-4,5-dihydro-1,2,4-triazolo-4-pheny-(substituted)-quinolin-2-one derivatives (3a-3t). Then condensation of (3a-3t) with phenacyl bromide provided series of fused heterocyclic derivatives of 4-phenyl-1-({6-phenyl-7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-3-yl}methyl)-(substituted)-1,2-dihydro quinolin-2-one (4a-4t). Structures of these newly synthesized derivatives were established by elemental analysis, FT-IR, 1H NMR and Mass spectroscopy. Final derivatives (4a-4t) were screened for their in vitro antibacterial and antifungal activities against the standard drugs Ampicillin and Fluconazole respectively. The compounds 4d, 4g and 4j showed potent activity against all the studied microbes. Particularly compounds substituted with halogen groups at para position of phenylquinoline ring exhibited significant antimicrobial activity against studied microbes. © 2015.


Mishra S.B.,United Institute of Pharmacy | Pandey H.,Allahabad University | Pandey A.C.,Allahabad University
Advances in Natural Sciences: Nanoscience and Nanotechnology | Year: 2013

Phyllanthus amarus (P. amarus) is commonly used for traditional Indian medicine and as dietary adjuncts for the treatment of numerous physiological disorders including hepatic disorders. Due to the poor water solubility of its major constituents such as lignans and flavonoids, its absorption upon oral administration could be limited. The present study was designed to evaluate and compare the hepatoprotective effects of the ethanolic extract of P. amarus (PAE) and its nanoparticles (PAN) on paracetamol induced acute liver toxicity in Sprague-Dawley rats. An oral dose of PAE at 125 and 250 mg kg-1 and PAN at 25 and 50 mg kg-1 showed a significant hepatoprotective effect relatively to the same extent (P < 0.001) by reducing levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and bile salts. These biochemical assessments were supported by rat hepatic biopsy examinations. Moreover, the results also indicated that the hepatoprotective effect of 50 mg kg-1 PAN was effectively better than 125 mg kg -1 PAE (P < 0.001), and an oral dose of PAN that is five times less than PAE could exhibit similar levels of outcomes. In conclusion, we suggest that the nanoparticles system can be applied to overcome other poorly water soluble herbal medicines and furthermore to decrease the treatment dosage. © 2013 Vietnam Academy of Science & Technology.


Pandey P.,United Institute of Pharmacy | Singh J.,Allahabad University
Der Pharma Chemica | Year: 2014

2-Amino-4-aryl thiazoles and their derivatives have long been used as precursors for the synthesis of biologically active molecules. Therefore new antifungal compounds IV(a-e) have been synthesized incorporating 2-Amino-4-aryl thiazole. The title compounds were synthesized through isolable intermediates. All the synthesized compounds have been characterized by their elemental analysis, 1HNMR and Mass spectra. The synthesized compounds were evaluated in vitro for their antifungal activity against the test fungi by poisoned food technique; at 1000, 100 and 10 ppm using Czapek's agar medium. A standard commercial fungicide Dithane M-45 was also tested under similar conditions for comparison. It was found that all the compounds were antifungal active but amongst them IVd displayed fungicidal action comparable with that of commercial fungicide Dithane M-45.


Mishra S.B.,United Institute of Pharmacy | Verma A.,Indigenous | Mukerjee A.,United Institute of Pharmacy | Vijayakumar M.,National Botanical Research Institute
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: The aim of the present study was to evaluate the possible antidiabetic effects of Amaranthus spinosus leaf extract (ASEt) against streptozotocin-nicotinamide induced diabetes & oxidative stress in albino rats. Methods: Experimental diabetes was induced by a single dose of STZ (60 mg/kg) administered by intraperitoneal way after the administration of nicotinamide (120mg/kg). The oxidative stress was measured by reduced glutathione (GSH) content and by enzymatic activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in liver and kidney. Biochemical observations were further substantiated with histological examination of pancreas, kidney and liver. Results: The increase in blood glucose with the decrease in GSH content and in enzymatic activities were the salient features observed in diabetic rats. Administration of ASEt (250 & 500 mg/kg bw/day, i.p) for 21 days caused a significant reduction in blood glucose in STZ-nicotinamide treated rats when compared with diabetic rats. Furthermore, diabetic rats treated with ASEt leaf extract showed a significant increase in the activities of both enzymatic and non-enzymatic antioxidants when compared to those of diabetic rats. Degenerative changes of pancreatic cells in STZ treated rats were minimized to near normal morphology by administration of ASEt leaf extract as evidenced by histopathological examination. Conclusion: Results clearly indicate that Amaranthus spinosus treatment attenuate hyperglycemia by decreasing oxidative stress and pancreatic cells damage which may be attributed to its antioxidative potential. © 2012 Asian Pacific Tropical Biomedical Magazine.


Mishra S.B.,United Institute of Pharmacy | Verma A.,Indigenous | Mukerjee A.,United Institute of Pharmacy | Vijayakumar M.,National Botanical Research Institute
Asian Pacific Journal of Tropical Medicine | Year: 2011

Objective: To evaluate the antihyperglycemic activity of leaves of Hyptis suaveolens using streptozotocin model. Methods: Hyptis suaveolens extract (HSE) 250 and 500 mg/kg body weight was administered orally to streptozotocin induced diabetes, once daily for 21 days. Results: A significant reduction in blood glucose was observed in diabetic animals treated with HSE at different doses when compared with diabetic rats. Levels of triglyceride, total cholesterol, low density lipoprotein, very low density lipoprotein were decreased while administering HSE at different doses, compared with their control values in diabetic animals. Conclusions: Our results show that HSE possesses significant antihyperglycemic activity which might be attributed to stimulating effects on glucose utilization and antioxidant enzyme. © 2011 Hainan Medical College.


Ahmed D.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Sharma M.,Jamia Hamdard University | Mukerjee A.,United Institute of Pharmacy | Ramteke P.W.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Kumar V.,Sam Higginbottom Institute of Agriculture, Technology and Sciences
BMC Complementary and Alternative Medicine | Year: 2013

Background: The present study was undertaken to evaluate the antihyperglycemic, antihyperlipidemic and hepatoprotective effect of a traditional unani formulation " Qurs Tabasheer" in streptozotocin (STZ) induced diabetic wistar rats. Up till now no study was undertaken to appraise the efficacy of " Qurs Tabasheer" in the diabetic rats. Qurs Tabasheer is a unani formulation restraining preparations from five various herbs namely Tukhme Khurfa (Portulaca oleracea seed), Gule Surkh (Rosa damascena flower), Gulnar (Punica granatum flower), Tabasheer (Bambusa arundinasia dried exudate on node), Tukhme Kahu (Lactuca sativa Linn seed).Methods: Effect of Qurs Tabasheer was assessed in STZ (60 mg/kg, i.p single shot) induced diabetic wistar rats. STZ produced a marked increase in the serum glucose, Total Cholesterol, LDL cholesterol, VLDL Cholesterol, Triglycerides and trim down the HDL level. We have weighed up the effect of Qurs Tabasheer on hepatic activity through estimating levels of various liver enzymes viz. Hexokinase, Glucose-6-Phosphatase and Fructose-1-6-biphosphatase in STZ diabetic wistar rats.Results: In STZ-induced diabetic wistar rats level of Hexokinase, and Glucose-6-Phosphatase was decreased to a significant level while the level of fructose-1-6-biphophatase was augmented. Therapy with Qurs Tabasheer for 28 days to STZ-induced diabetic rats significantly reduces the level of serum glucose, total cholesterol, triglycerides, glucose-6-phosphatase and fructose-1-6-biphosphatase, while magnitude of HDL cholesterol and hexokinase was amplified.Conclusion: Antihyperglycemic, antihyperlipidemic activity of Qurs Tabasheer extract in STZ- induced wistar rats was found to be more effective than standard oral hypoglycemic drug Glimepiride. © 2013 Ahmed et al.; licensee BioMed Central Ltd.


Mishra S.B.,United Institute of Pharmacy | Verma A.,Sam Higginbottom Institute of Agriculture, Technology and Sciences | Vijayakumar M.,Himalaya
Biomarkers and Genomic Medicine | Year: 2013

This study aimed to evaluate the antidiabetic activity of Strychnos potatorum seeds in streptozotocin-nicotinamide-induced diabetes in experimental animals. Noninsulin-dependent diabetes mellitus (NIDDM) was induced in overnight fasted rats by an intraperitoneal injection (i.p.) of 60mg/kg streptozotocin (STZ) and, after a 15-minute interval, 120mg/kg of nicotinamide. S. potatorum extract 200mg/kg or 400mg/kg body weight was administered orally to the rats once daily for 21 days. The blood glucose level was assessed by a glucometer. The serum levels of cholesterol, triglycerides, and total lipid were determined by using diagnostic kits. Measurement of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), reduced glutathione (GSH), and glutathione peroxidase (GPx) were determined to ascertain the antioxidant activity. A significant reduction in the blood glucose level was observed in diabetic animals treated with the different doses of the extract, compared to untreated diabetic rats. The treatment with the extract significantly increased the levels of GSH, GPx, GST, CAT, and SOD in the drug-treated group to levels comparable to the levels in the diabetic control group. The result of this study thus shows that 50% of the ethanolic extract at different doses possesses significant antidiabetic activity and potent antioxidant potential in diabetic conditions. © 2013 .


Das T.,Girijananda Chowdhury Institute of Pharmaceutical Science | Das B.,Government Ayurvedic College | Saha D.,Girijananda Chowdhury Institute of Pharmaceutical Science | Mishra S.B.,United Institute of Pharmacy
Journal of Applied Pharmaceutical Science | Year: 2015

Objective: The primary objective of the present investigation is to evaluate the antihyperglycemic activity of the 50% ethanolic extract of the Tecomella undulata Seem. (TUE) bark in the streptozotocin induced diabetic rats. Methods: Single intraperitoneal injection (i.p.) of streptozotocin (60 mg/kg body weight) was used for induction of diabetes is wistar albino rats. The induction of diabetes was confirmed after 3 days after STZ injection and rats with fasting blood glucose levels greater than 200 mg/dl were considered to be diabetic used in the experiment. TUE at a once a daily dose of 250 mg/kg, and 500 mg/kg, p.o. along with glibenclamide 600μg/kg, p.o. was also given for 21 days. On the 21st day rats from all the groups fasted overnight and the blood was collected from the puncturing the retro orbit of the eye under mild ether anesthetic condition. The oral acute toxicity studies did not show any toxic effect till the dose at 2000 mg/kg. Results: The statistical data indicated that the different doses of the TUE significantly decreases the level of fasting blood glucose, total cholesterol, triglycerides, low density lipoprotein and increase high density lipoprotein in STZ induced diabetic rats. This result indicated that T. undulata extract can protect pancreatic ß-cells from STZ-induced damage which is confirmed by the results of the histopathological examination of the pancreas. Conclusion: Our investigation has clearly indicated that the bark extract of Tecomella undulata Seem. showed remarkable antihyperglycemic activity due to its possible systematic effect involving in the pancreatic and extra pancreatic mechanism. Moreover, the antihyperlipidemic activity was exerted possible by lowering the higher level of lipid profile. © 2015 Trishna Das et al.


Saha D.,Girijananda Chowdhury Institute of Pharmaceutical Science | Ghosh S.K.,Dibrugarh University | Das T.,Girijananda Chowdhury Institute of Pharmaceutical Science | Mishra S.B.,United Institute of Pharmacy
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: To investigate the hypoglycemic and antihyperlipidemic effect of a successive ethanolic extract of Adiantum caudatum (EEAC) whole plant in alloxan induced diabetic rats. Methods: Diabetes was induced in Wistar albino rats by the administration of alloxan (140 mg/kg b. w., i.p.). EEAC (200 mg/kg b. w., p.o.) was administered to diabetic rats for 21 days in alloxan induced diabetic rats. The effect of EEAC on blood glucose and body weight was studied in alloxan induced diabetic rats. All these effects were compared with glibenclamide (10 mg/kg b. w., p.o.) as a reference antidiabetic drug. Results: The administration of EEAC (200 mg/kg b. w., p.o.) resulted in a significant decrease in blood glucose level and a significant increase in body weight in alloxan induced diabetic rats. Furthermore, EEAC showed antihyperlipidemic activity as evidenced by a significant decrease in serum total cholesterol and triglyceride levels in alloxan induced diabetic rats. Conclusion: The results suggest that the EEAC possess a promising hypoglycemic effect in alloxan induced diabetic rats. © 2016, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


Ghosh S.,United Institute of Pharmacy | Mandal M.K.,United Institute of Pharmacy
Journal of the Indian Chemical Society | Year: 2013

A series of 1,2,4-triazoIophenyIquinoiine derivatives were synthesized by cyclisation method. Synthesized molecules were characterized by spectral and elemental analysis. These newly synthesized molecules were screened for their in vitro antioxidant activity. Some of the synthesized compounds showed potent antioxidant activity.

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