Chan I.H.S.,Chinese University of Hong Kong |
Lee D.L.Y.,Chinese University of Hong Kong |
Ho O.Y.M.,The United Christian Hospital |
Wong E.W.Y.,Chinese University of Hong Kong |
And 7 more authors.
Clinica Chimica Acta | Year: 2010
Background: Early growth response-1 (Egr-1) is expressed in human airways and its polymorphisms have been associated with total IgE and atopy in asthmatic patients. We investigated the effects of Chinese-tagging single nucleotide polymorphism (SNP) of Egr-1 and its mRNA expression on allergic rhinitis (AR) traits. Methods: Among 214 Chinese AR adults and 259 controls, tag SNP -4071 A → G was genotyped and mRNA expression in peripheral blood was quantified by real-time PCR. Results: Egr-1 mRNA expression was significantly higher in patients than controls (median of 0.23 vs 0.15 fold GAPDH expression; p < 0.001). Its expression was not associated with - 4071 polymorphism. However, significant correlations were found between - 4071 A → G with increased plasma total IgE (p = 0.028) and atopy (p = 0.030) in patients. Logistic regression confirmed the association (p = 0.034) with age and gender adjusted. Patients homozygous for the A allele had a 2.3-fold and 1.9-fold risks, respectively of having increased plasma total IgE and atopy than those G allele carriers. Conclusions: We showed high levels of Egr-1 mRNA expression and demonstrated a significant association of polymorphism with increased plasma total IgE and atopy in AR patients. It may be useful to explore the pharmacogenetics of Egr-1 inhibitors. © 2009 Elsevier B.V. All rights reserved.