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Gheith S.,Service dHygiene Hospitaliere | Gheith S.,Laboratoire Of Parasitologie Mycologie | Saghrouni F.,Unite de recherche UR 04SP24 | Bannour W.,Service dHygiene Hospitaliere | And 10 more authors.
Mycopathologia | Year: 2014

Although scarce, available data suggest that the epidemiology of invasive aspergillosis (IA) in North Africa differs from northern countries, where more than 80 % is caused by Aspergillus fumigatus. This study aimed at describing the epidemiology of IA in the region of Sousse, Tunisia, and at assessing the usefulness of the available diagnostic tools. For 2 years, clinical and mycological data were prospectively collected from 175 neutropenia episodes of 91 patients hospitalised in the haematology department at the Farhat Hached hospital in Sousse (Tunisia). Screening for galactomannan antigen was positive in 40 % of neutropenia episodes; Aspergillus PCR was positive in 42 % of the tested sera. Nine patients were classified as probable and two as possible IA according to the EORTC/MSG criteria. Twelve patients who prematurely died, had no CT scan and could not be classified. Fifty-six Aspergillus spp. were isolated in 53 (6.5 %) sputa collected from 35 (20 %) patients. The following species were identified with MALDI-TOF mass spectrometry and DNA sequencing: A. niger, 35 %; A. flavus, 38 %; A. tubingensis, 19 %; A. fumigatus, 4 %; A. westerdijkiae, 2 % and A. ochraceus, 2 %. Our findings highlight the epidemiological features of IA in Tunisia, which is characterised by the predominance of Aspergillus spp. from sections Nigri and Flavi. © 2014 Springer Science+Business Media Dordrecht.

Gheith S.,Unite de recherche UR 04SP24 | Gheith S.,Laboratoire Of Parasitologie Mycologie | Saghrouni F.,Laboratoire Of Parasitologie Mycologie | Bannour W.,Unite de recherche UR 04SP24 | And 6 more authors.
Nutrition Clinique et Metabolisme | Year: 2012

Objective: The aim of our study was to evaluate the level of fungal contamination of food served to patients in the hematology unit of Farhat Hached hospital, Sousse (Tunisia). Methods: We analyzed 159 food served to 90 patients during the post-chemotherapy aplasia period. Results: The overall rate of fungal contamination was 66.6%. Fruits and green salads' contamination was very high (90 and 100%, respectively). Aspergillus species were the most frequent (48.4%) followed by Candida (25.1%), Penicillium (22%), Rhizopus (17.6%) and Cladosporium (17%). The presence of Aspergillus on fruits (66.6%) represents a real source of contamination by inhalation of spores and the presence of Candida (25.1%) in any type of food is a source of gastrointestinal colonization. Conclusion: The diet of neutropenic patients should be submitted to a strict surveillance to reduce the risk of fungal infections of food. © 2012 Elsevier Masson SAS.

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