Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20

Tunis, Tunisia

Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20

Tunis, Tunisia

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Debouba M.,Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20 | Debouba M.,Institute Superieur Of Biologie Appliquee Of Medenine | Suzuki A.,French National Institute for Agricultural Research | Boutet S.,French National Institute for Agricultural Research | And 2 more authors.
Acta Botanica Gallica | Year: 2010

Tomato seedlings were exposed to 100 mM NaCI during 10 days. NaCI stress led to about 40% and 20% decrease in leaf and root biomass, respectively. Accumulation of Na+ and Cl" ions was concomitant with NH 4 + contents increase in stressed leaves and roots. While total aminoacids contents rapidly increased by salt in both organs, they recovered control value in the leaves at the end of treatment In leaves, NaCl stress increased proline and serine contents, lately associated with accumulation of y-amincibutyric and asparagine. Slat-treated roots showed 2-fold higher contents of serine, glycine, glutamina and GABA. Asparagine became the most abundant aminoacid, accounting for 26% of total aminoacids in the treated roots. These disturbances in the aminoacids composition reflect a new distribution of internal nitrogen compounds, building up an aminoacid pool with high N to C ratio and avoiding thus an ammoniacal intoxication. Proline, Asn and GABA are useful for nitrogen transport to developing tissues and protect against osmotic and oxidative stresses during salt stress.


Debouba M.,Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20 | Debouba M.,Gabes University | Dguimi H.M.,Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20 | Ghorbel M.,Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20 | And 2 more authors.
Journal of Plant Physiology | Year: 2013

Key steps in nitrate nutrition and assimilation were assessed over two weeks in control and 100. mM NaCl-exposed Arabidopsis thaliana (Columbia) plants. The data showed that NaCl stress lowered nitrate contents in both leaves and roots. While NaCl stress decreased ammonium contents in leaves, it increased the contents in roots at the end of treatment. A survey of transcript levels of NIA1 (At1g77760) and NIA2 (At1g37130) and nitrate reductase (NR, EC 1.6.1.6) activity in the leaves and roots suggested a major role of NIA2 rather than NIA1 in the regulation of NR by salt stress. A drop in mRNA levels for GLN2 (At5g35630) and GLN1;2 (At1g66200) by salt was associated with a similar inhibition of glutamine synthetase (GS, EC 6.3.1.2) activity in the leaves. In the roots, NaCl stress was found to enhance mRNA levels of GLN2 and cytosolic-encoding genes (GLN1;1 (At5g37600) and GLN1;2). © 2012 Elsevier GmbH.


PubMed | Unite de Recherche Nutrition et Metabolisme Azotes et Proteines de Stress 99 UR C 09 20
Type: Journal Article | Journal: Journal of plant physiology | Year: 2012

Key steps in nitrate nutrition and assimilation were assessed over two weeks in control and 100mM NaCl-exposed Arabidopsis thaliana (Columbia) plants. The data showed that NaCl stress lowered nitrate contents in both leaves and roots. While NaCl stress decreased ammonium contents in leaves, it increased the contents in roots at the end of treatment. A survey of transcript levels of NIA1 (At1g77760) and NIA2 (At1g37130) and nitrate reductase (NR, EC 1.6.1.6) activity in the leaves and roots suggested a major role of NIA2 rather than NIA1 in the regulation of NR by salt stress. A drop in mRNA levels for GLN2 (At5g35630) and GLN1;2 (At1g66200) by salt was associated with a similar inhibition of glutamine synthetase (GS, EC 6.3.1.2) activity in the leaves. In the roots, NaCl stress was found to enhance mRNA levels of GLN2 and cytosolic-encoding genes (GLN1;1 (At5g37600) and GLN1;2).

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