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Bachrouch O.,Unite de Recherche de Physico Chimie Moleculaire URPCM | Bachrouch O.,Center de Biotechnologie de la Technopole de Borj-Cedria | Msaada K.,Center de Biotechnologie de la Technopole de Borj-Cedria | Wannes W.A.,Center de Biotechnologie de la Technopole de Borj-Cedria | And 5 more authors.
Plant Biosystems | Year: 2015

In this study, the composition and antioxidant activity of Pistacia lentiscus L. leaf essential oil (EO) from four Tunisian localities were investigated. The EO yields (%, w/w) of P. lentiscus leaf were 0.009% in Oued El Bir, 0.02% in Jebel Mansour, 0.007% in Siliana and 0.01% in Tabarka. The main compounds of Oued El Bir EO were terpinene-4-ol (41.24%) and α-terpineol (7.31%), whereas those of Jebel Mansour were α-pinene (9.48%), limonene (19.11%) and α-phellandrene (3.20%). In Siliana samples, terpinene-4-ol (23.32%), β-caryophyllene (22.62%) and α-terpineol (7.12%) were the main compounds. For P. lentiscus L. from Tabarka, α-terpineol (9.79%) and β-caryophyllene (38.33%) were the major constituents. Three chemotypes of P. lentiscus EO were identified for the first time in Tunisia. P. lentiscus EOs were also screened for their antioxidant activities. The 1,1-diphenyl-2-picrylhydrazyl assay gives an IC50 range value of 60–110 μg/mL for all the samples studied. EOs from different Tunisian localities showed lower β-carotene bleaching activity than butylated hydroxytoluene and butylated hydroxyanisol. P. lentiscus EO presents a very low capacity to chelate ferrous irons (IC50 = 80.8–104 μg/mL) and no metal chelating activity was recorded. © 2013, Società Botanica Italiana.


Mediouni-Ben Jemaa J.,French National Institute for Agricultural Research | Bachrouch O.,Unite de Recherche de Physico Chimie Moleculaire URPCM | Bachrouch O.,Center de Biotechnologie de la Technopole de Borj-Cedria | Marzouk B.,Unite de Recherche de Physico Chimie Moleculaire URPCM | And 3 more authors.
Acta Horticulturae | Year: 2010

During the past few decades, the need for safer means of pest management have become crucial due to the harmful effects of synthetic insecticides on the environment, problems related to pests' resurgence and resistance to pesticide and risk for users. Therefore, a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides was developed. In this study, we report first investigations on chemical constituents and fumigant toxicity of Pistacia lentiscus L. (Anacardiacea) essential oil against 1-7 day-old adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Lasioderma serricorne (F.) (Coleoptera: Anobiidae). The major compounds of the essential oil determined using the GC and the GC-MS were: α-phellandrene (3.20%), α-pinene (9.48%) and limonene (19.11%). Oil amounts tested were 5 and 45 μl in each 44 ml Plexiglas bottle with capacity corresponding to concentrations 114 and 1023 μl/L air. Great differences in insect mortality were observed within insect species, oil concentrations and exposure time. The fumigant toxicity potential of P. lentiscus on L. serricorne was higher (LC50= 8.44 μl/L, LC95= 43.68 μl/L) than T. castaneum (LC50=28.03 μl/L, LC95=63.46 μl/L). The results suggested that P. lentiscus essential oil may have potential as a control agent against these two stored product beetles.

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