Unite de Biochimie Macromoleculaire et Genetique

Gafsa, Tunisia

Unite de Biochimie Macromoleculaire et Genetique

Gafsa, Tunisia

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Beltifa A.,Unite de Biochimie Macromoleculaire et Genetique | Beltifa A.,University of Monastir | Borgi M.A.,Unite de Biochimie Macromoleculaire et Genetique | Ferieni A.,University of Sfax | And 3 more authors.
Environmental Science and Pollution Research | Year: 2016

In the present study, adult, healthy male Wistar rats (120 ± 10 g) were pre-treated by intragastric administration of cerium chloride (CeCl3) 10 mg/kg (BW) each day during 60 days. Control animal were treated with omega 3, a polyunsaturated fatty acid (ω-3), by an intragastric administration at 10 mg/kg of BW for 60 days. Our results showed that CeCl3-induced alterations in all tested oxidative stress markers. In fact, CeCl3-induced the increase the level of both the creatinine concentration and the expression of lactate dehydrogenase, alkaline phosphatase, and transaminase activities in serum. On the other hand, CeCl3 significantly increased the levels of lipid peroxidation in the renal and hepatic tissues. The capacity of CeCl3 to generate reactive oxygen species (ROS) could explain his ability to induce morphological alterations, such as centrilobular hemorrhage, hepatic necrosis, and vacuolization of the cytoplasm in hepatic tissues, and the atrophy of the glomerulus and dilatation of urinary space in renal tissues. However, omega 3, after gastric administration, reduced significantly the toxic effect caused by CeCl3 according to his high ability to scavenge ROS. The present study indicates that omega 3 is a significant compound with protective activity against intoxication with heavy metal, the cerium, and thus may be useful for chemoprevention. © 2016 Springer-Verlag Berlin Heidelberg


Alimi H.,Unite de Biochimie Macromoleculaire et Genetique | Alimi H.,University of Carthage | Hfaiedh N.,University of Sfax | Bouoni Z.,Unite de Biochimie Macromoleculaire et Genetique | And 2 more authors.
Environmental Toxicology and Pharmacology | Year: 2011

The Opuntia ficus indica f. inermis methanolic flowers extract (OMFE) was phytochemical studied, in vitro tested for their potential antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, linoleic acid peroxidation assays and in vivo evaluated for its ability to prevent ethanol-induced gastric ulcer in rats. The OMFE was rich in polysaccharide, phenolics and flavonoids contents and exhibited a moderate in vitro antioxidant activity when compared with (+)-catechin and ascorbic acid. Pre-treatment with OMFE at oral doses 250, 500 and 1000. mg/kg body weight was found to provide a dose-dependent protection against ethanol-induced gastric ulcer by averting the deep necrotic lesions of the gastric epithelium, by preserving normal antioxidant enzymes activities, by inhibiting the lipid peroxidation, the oxidation of protein and the DNA fragmentation in gastric mucosa. The antiulcerogenic activity of OMFE might be due to a possible synergistic antioxidant and antihistaminic-like effects. © 2011 Elsevier B.V.


Alimi H.,Unite de Biochimie Macromoleculaire et Genetique | Alimi H.,University of Carthage | Hfaeidh N.,Unite de Biochimie Macromoleculaire et Genetique | Bouoni Z.,Unite de Biochimie Macromoleculaire et Genetique | And 2 more authors.
Experimental and Toxicologic Pathology | Year: 2013

The aim of the present study was to investigate the efficacy of Opuntia ficus indica f. inermis fruit juice (OFIj) on reversing oxidative damages induced by chronic ethanol intake in rat erythrocytes. OFIj was firstly analyzed with HPLC for phenolic and flavonoids content. Secondly, 40 adult male Wistar rats were equally divided into five groups and treated for 90 days as follows: control (C), ethanol-only 3. g/kg body weight (b.w) (E), low dose of OFIj 2. ml/100. g b.w. +. ethanol (Ldj. +. E), high dose of OFIj 4. ml/100. g b.w. +. ethanol (Hdj. +. E), and only a high dose of OFIj 4. ml/100. g b.w (Hdj). HPLC analysis indicated high concentrations of phenolic acids and flavonoids in OFIj. Ethanol treatment markedly decreased the activities of erythrocyte superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px), and the level of reduced glutathione (GSH). Changes in the erythrocyte's antioxidant ability were accompanied by enhanced oxidative modification of lipids (increase of malondialdeyde level) and proteins (increase in carbonyl groups). Interestingly, pre-administration of either 2. ml/100. g b.w or 4. ml/100. g b.w of OFIj to ethanol-intoxicated rats significantly reversed decreases in enzymatic as well as non enzymatic antioxidants parameters in erythrocytes. Also, the administration of OFIj significantly protected lipids and proteins against ethanol-induced oxidative modifications in rat erythrocytes. The beneficial effect of OFIj can result from the inhibition of ethanol-induced free radicals chain reactions in rat erythrocytes or from the enhancement of the endogenous antioxidants activities. © 2011 Elsevier GmbH.


Alimi H.,Unite de Biochimie Macromoleculaire et Genetique | Alimi H.,University of Carthage | Hfaiedh N.,Unite de Biochimie Macromoleculaire et Genetique | Bouoni Z.,Unite de Biochimie Macromoleculaire et Genetique | And 4 more authors.
Phytomedicine | Year: 2010

Opuntia ficus indica f. inermis methanolic root extract (ORE) was investigated for phenolic and flavonoids contents, in vitro evaluated for DPPH radical scavenging activity, reducing power and in vivo tested for its gastro-protective ability against 80% ethanol induced ulcer in rats. Phytochemical test of ORE were positive for phenolic and flavonoid contents. DPPH radical scavenging activity and reducing power of ORE showed an EC50 of 118.65±2.51μg/ml and 300μg/ml respectively. In vivo the pre-treatment of rats with ranitidine (50mg/kg) and 200, 400, and 800mg/kg doses of ORE significantly (p<0.05) reduced the 80% ethanol induced-ulcer lesion, with a rate of 82.68%, 49.21%, 83.13%, and 92.59% respectively, and prevented the depletion of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total glutathione (GSH), and inhibited the increase of myeloperoxidase (MPO) and malondialdehyde (MDA) in rat stomach tissues when compared with ethanol group. Also pre-treateatment with ORE marked a dose-dependent attenuation of histopathology changes induced by ethanol. Phenolic and flavonoids wealth, radical scavenging activity, and reducing power, have been implicated for antiulcer property of ORE. © 2010 Elsevier GmbH.


PubMed | Unite de Biochimie Macromoleculaire et Genetique
Type: Journal Article | Journal: Environmental toxicology and pharmacology | Year: 2011

The Opuntia ficus indica f. inermis methanolic flowers extract (OMFE) was phytochemical studied, in vitro tested for their potential antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH), reducing power, linoleic acid peroxidation assays and in vivo evaluated for its ability to prevent ethanol-induced gastric ulcer in rats. The OMFE was rich in polysaccharide, phenolics and flavonoids contents and exhibited a moderate in vitro antioxidant activity when compared with (+)-catechin and ascorbic acid. Pre-treatment with OMFE at oral doses 250, 500 and 1000 mg/kg body weight was found to provide a dose-dependent protection against ethanol-induced gastric ulcer by averting the deep necrotic lesions of the gastric epithelium, by preserving normal antioxidant enzymes activities, by inhibiting the lipid peroxidation, the oxidation of protein and the DNA fragmentation in gastric mucosa. The antiulcerogenic activity of OMFE might be due to a possible synergistic antioxidant and antihistaminic-like effects.


PubMed | Unite de Biochimie Macromoleculaire et Genetique
Type: Journal Article | Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology | Year: 2010

Opuntia ficus indica f. inermis methanolic root extract (ORE) was investigated for phenolic and flavonoids contents, in vitro evaluated for DPPH radical scavenging activity, reducing power and in vivo tested for its gastro-protective ability against 80% ethanol induced ulcer in rats. Phytochemical test of ORE were positive for phenolic and flavonoid contents. DPPH radical scavenging activity and reducing power of ORE showed an EC(50) of 118.652.51 g/ml and 300 g/ml respectively. In vivo the pre-treatment of rats with ranitidine (50 mg/kg) and 200, 400, and 800 mg/kg doses of ORE significantly (p<0.05) reduced the 80% ethanol induced-ulcer lesion, with a rate of 82.68%, 49.21%, 83.13%, and 92.59% respectively, and prevented the depletion of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), total glutathione (GSH), and inhibited the increase of myeloperoxidase (MPO) and malondialdehyde (MDA) in rat stomach tissues when compared with ethanol group. Also pre-treatment with ORE marked a dose-dependent attenuation of histopathology changes induced by ethanol. Phenolic and flavonoids wealth, radical scavenging activity, and reducing power, have been implicated for antiulcer property of ORE.


PubMed | Unite de Biochimie Macromoleculaire et Genetique
Type: Journal Article | Journal: Alcohol (Fayetteville, N.Y.) | Year: 2012

Juice from the fruit of the cactus Opuntia ficus indica is claimed to possess several health-beneficial properties. The present study was carried out to determine whether O. ficus indica f. inermis fruit extract might have a protective effect upon physiological and morphological damages inflicted to erythrocytes membrane by chronic ethanol poisoning, per os, in rat. Chemical analysis of the extract revealed the presence of polyphenols, flavonoids, ascorbic acid, carotenoids, and betalains. Ethanol administration (3 g/kg b.w, per day for 90 days) induced an increase of malondialdehyde (MDA) and carbonylated proteins levels and a decrease of glutathione (GSH) level in erythrocyte. Ethanol administration also reduced the scavenging activity in plasma and enhanced erythrocytes hemolysis, as compared to control rats. In addition, ethanol intake increased the erythrocyte shape index by +895.5% and decreased the erythrocyte diameter by -61.53% as compared to controls. In animals also given prickly pear juice during the same experimental period, the studied parameters were much less shifted. This protective effect was found to be dose-dependent. It is likely that the beneficial effect of the extract is due to the high content of antioxidant compounds.

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