Unitat de Toxicologia Experimental i Ecotoxicologia

Eixample, Spain

Unitat de Toxicologia Experimental i Ecotoxicologia

Eixample, Spain
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Porredon Guarch C.,Unitat de Toxicologia Experimental i Ecotoxicologia | Ramos Lopez D.,Unitat de Toxicologia Experimental i Ecotoxicologia | Serret Salse J.,Unitat de Toxicologia Experimental i Ecotoxicologia | Gonzalez Linares J.,Unitat de Toxicologia Experimental i Ecotoxicologia | And 2 more authors.
Revista de Toxicologia | Year: 2014

The increasing annual production of nanomaterials enhances the human and environmental exposure, as well as the possible impact on public concern. In order to regulate the production, international organizations such as the Organization for Economic Cooperation and Development (OECD) and the European Comission through the REACH regulation have established some measures to assess its health and environmental safety. This review tries to analyze these proposed measures according to the standard test used in toxicology, the different classification of nanomaterials and the main mechanisms of toxicity known for nanomaterials. Resulting from this analysis, it is considered convenient to continue the development of specific tests for nanomaterial evaluation, as the measures established by the organizations of reference are not enough to establish standard basis for testing nanomaterials. This is mainly due to the huge diversity of manufactured nanomaterials and the importance of its handling, techniques and experimental systems chosen in the toxicity results. © 2014, Asociacion Espanola de Toxicologia. All rights reserved.

Serra-Cobo J.,University of Barcelona | Lopez-Roig M.,University of Barcelona | Sanchez L.P.,CIBER ISCIII | Nadal J.,University of Barcelona | And 3 more authors.
PLoS ONE | Year: 2013

Bats have been proposed as major reservoirs for diverse emerging infectious viral diseases, with rabies being the best known in Europe. However, studies exploring the ecological interaction between lyssaviruses and their natural hosts are scarce. This study completes our active surveillance work on Spanish bat colonies that began in 1992. Herein, we analyzed ecological factors that might affect the infection dynamics observed in those colonies. Between 2001 and 2011, we collected and tested 2,393 blood samples and 45 dead bats from 25 localities and 20 bat species. The results for dead confirmed the presence of EBLV-1 RNA in six species analyzed (for the first time in Myotis capaccinii). Samples positive for European bat lyssavirus-1 (EBLV-1)-neutralizing antibodies were detected in 68% of the localities sampled and in 13 bat species, seven of which were found for the first time (even in Myotis daubentonii, a species to date always linked to EBLV-2). EBLV-1 seroprevalence (20.7%) ranged between 11.1 and 40.2% among bat species and seasonal variation was observed, with significantly higher antibody prevalence in summer (July). EBLV-1 seroprevalence was significantly associated with colony size and species richness. Higher seroprevalence percentages were found in large multispecific colonies, suggesting that intra- and interspecific contacts are major risk factors for EBLV-1 transmission in bat colonies. Although bat-roosting behavior strongly determines EBLV-1 variability, we also found some evidence that bat phylogeny might be involved in bat-species seroprevalence. The results of this study highlight the importance of life history and roost ecology in understanding EBLV-1-prevalence patterns in bat colonies and also provide useful information for public health officials.

Giannicchi I.,CNR Methodological Chemistry Institute | Brissos R.,University of Barcelona | Ramos D.,Unitat de Toxicologia Experimental i Ecotoxicologia | Lapuente J.D.,Unitat de Toxicologia Experimental i Ecotoxicologia | And 3 more authors.
Inorganic Chemistry | Year: 2013

The synthesis, characterization, and luminescent properties of a series of 5,5′-X-substituted salophen ligands, X= OCH3, Br, and NO 2, and the corresponding Zn(II) complexes are reported here. Their biological activity has been analyzed and related to the different Lewis acid character of the complexes. In vitro studies (AFM and absorption and emission titrations) show that the strongest interaction with free plasmid DNA is observed for 5,5′-dinitro-substituted Zinc-salophen complex 3b. Semiempirical theoretical calculations together with redox potential measurements suggest that this might be interpreted as a direct consequence of this compound having the hardest Lewis acid character. Cellular uptake and cytotoxicity studies undertaken with these metal complexes show that they enter the cells but are not cytotoxic. © 2013 American Chemical Society.

Llado S.,University of Barcelona | Solanas A.M.,University of Barcelona | de Lapuente J.,Unitat de Toxicologia Experimental i Ecotoxicologia | Borras M.,Unitat de Toxicologia Experimental i Ecotoxicologia | Vinas M.,GIRO Joint Research Unit IRTA UPC
Science of the Total Environment | Year: 2012

A diversified approach involving chemical, microbiological and ecotoxicity assessment of soil polluted by heavy mineral oil was adopted, in order to improve our understanding of the biodegradability of pollutants, microbial community dynamics and ecotoxicological effects of various bioremediation strategies. With the aim of improving hydrocarbon degradation, the following bioremediation treatments were assayed: i) addition of inorganic nutrients; ii) addition of the rhamnolipid-based biosurfactant MAT10; iii) inoculation of an aliphatic hydrocarbon-degrading microbial consortium (TD); and iv) inoculation of a known hydrocarbon-degrading white-rot fungus strain of Trametes versicolor. After 200. days, all the bioremediation assays achieved between 30% and 50% total petroleum hydrocarbon (TPH) biodegradation, with the T. versicolor inoculation degrading it the most. Biostimulation and T. versicolor inoculation promoted the Brevundimonas genus concurrently with other α-proteobacteria, β-proteobacteria and Cytophaga-Flexibacter-Bacteroides (CFB) as well as Actinobacteria groups. However, T. versicolor inoculation, which produced the highest hydrocarbon degradation in soil, also promoted autochthonous Gram-positive bacterial groups, such as Firmicutes and Actinobacteria. An acute toxicity test using Eisenia fetida confirmed the improvement in the quality of the soil after all biostimulation and bioaugmentation strategies. © 2012 Elsevier B.V.

Brissos R.,University of Barcelona | Ramos D.,Unitat de Toxicologia Experimental i Ecotoxicologia | Lima J.C.,New University of Lisbon | Mihan F.Y.,CNR Methodological Chemistry Institute | And 4 more authors.
New Journal of Chemistry | Year: 2013

The biological activity of two fluorescent Zn(ii)-salophen derivatives has been evaluated. In vitro studies (AFM, emission and UV-vis titration with ethidium bromide and cell growth inhibition) show different mechanisms of interaction with DNA. It has been observed that these compounds enter the cells. Comet assays (with cultured fibroblast cells) have revealed that cellular uptake occurs without damaging the DNA strands. Preliminary studies carried out with living cells have shown IC50 values in a millimolar range, indicative of a non-cytotoxic behaviour. This fact could be understood by confocal microscopy co-localization studies with living cell internalization that have shown that, in fact, the compounds seem to enter the cells but not the nucleus under in vivo conditions. © 2013 The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.

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