Entity

Time filter

Source Type


Rodriguez-Rodriguez C.E.,Unitat de Biocatalisi Aplicada associada al IQAC CSIC UAB | Marco-Urrea E.,Autonomous University of Barcelona | Caminal G.,Unitat de Biocatalisi Aplicada associada al IQAC CSIC UAB
Bioresource Technology | Year: 2010

Growth and activity of the white-rot fungus Trametes versicolor on sewage sludge were assessed in bioslurry and solid-phase systems. Bioslurry cultures with different loads of sludge (10%, 25% and 38%, w/v) were performed. A lag phase of at least 2 d appeared in the 25 and 38%-cultures, however, the total fungal biomass was higher for the latter and lower for the 10%-culture after 30 d, as revealed by ergosterol determination. Detectable laccase activity levels were found in the 10 and 25%-cultures (up to 1308 and 2588 AU L-1, respectively) while it was negligible in the 38%-culture. Important levels of ergosterol and laccase were obtained over a 60 d period in sludge solid-phase cultures amended with different concentrations of wheat straw pellets as lignocellulosic bulking material. Degradation experiments in 25%-bioslurry cultures spiked with naproxene (NAP, analgesic) and carbamazepine (CBZ, antiepileptic) showed depletion of around 47% and 57% within 24 h, respectively. Complete depletion of NAP and around 48% for CBZ were achieved within 72 h in sludge solid cultures with 38% bulking material. CBZ degradation is especially remarkable due to its high persistence in wastewater treatment plants. Results showed that T. versicolor may be an interesting bioremediation agent for elimination of emerging pollutants in sewage sludge. © 2009 Elsevier Ltd. All rights reserved. Source


Marco-Urrea E.,Autonomous University of Barcelona | Perez-Trujillo M.,Servei de Ressonancia Magnetica Nuclear | Blanquez P.,Autonomous University of Barcelona | Vicent T.,Autonomous University of Barcelona | Caminal G.,Unitat de Biocatalisi Aplicada associada al IQAC CSIC UAB
Bioresource Technology | Year: 2010

The white-rot fungus Trametes vesicolor degraded naproxen (10 mg L-1) in a liquid medium to non- detectable levels after 6 h. When naproxen was added in the range of concentrations typically found in the environment (55 μgL-1), it was almost completely degraded (95%) after 5 h. In vitro degradation experiments with purified laccase and purified laccase plus mediator 1-hydroxybenzotriazol showed slight and almost complete naproxen degradation, respectively. A noticeable inhibition on naproxen deg- radation was also observed when the cytochrome P450 inhibitor 1-aminobenzotriazole was added to the fungal cultures. These data suggest that both enzymatic systems could play a role in naproxen degrada- tion. 2-(6-hydroxynaphthalen-2-yl)propanoic acid and 1-(6-methoxynaphthalen-2-yl)ethanone were structurally elucidated by HPLC-DAD-MS and NMR as degradation intermediates of naproxen. After 6 h of incubation, both parent compound and intermediates disappeared from the medium. The non-toxicity of the treated medium was confirmed by Microtox test. © 2009 Elsevier Ltd. All rights reserved. Source

Discover hidden collaborations