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Santa Cristina e Bissone, Italy

Buoro S.,Unita Operativa Laboratorio Of Analisi Chimico Cliniche | Esposito S.A.,Unita Operativa Laboratorio Of Analisi Chimico Cliniche | Ottomano C.,Unita Operativa di Diagnostica Ematochimica | Alessio M.G.,Unita Operativa Laboratorio Of Analisi Chimico Cliniche | And 6 more authors.
Biochimica Clinica

This study was planned to assess the diagnostic performance of the automated urine particle analyzer Sysmex UF-1000i for the rapid screening of cerebrospinal fluid (CSF) in patients with suspected meningitis. Cytometric analyses with either optical microscopy (OM) or UF-1000i, along with assessment of glucose and protein in CSF, were performed on 101 consecutive CSF of patients with suspected meningitis. In 50 out of 101 samples, cultural analysis was also performed with different culture media. Four different diagnostic combination were developed, with different mix of the tested parameters. A high correlation was found between OM and UF-1000i (r=0.99; mean bias, -4.9/μL). The diagnostic agreement was 0.90 in adults and 0.97 in children. The diagnostic agreement between CSF culture and bacterial count by UF-1000i was 0.98, with 1.00 sensitivity and 0.98 specificity. Results showed that the diagnostic combination based on CSF glucose and total proteins, cytometric analysis (leukocyte count ± neutrophilia) and bacterial count on UF1000i exhibited the best performance when compared with microbiological examination (area under ROC curve, 1.00). In conclusion, the results of this study show that the combination of two rapid clinical chemistry tests such as glucose and total proteins with UF-1000i analysis could represent a valid approach for supporting more complex analyses or even for replacing OM and CSF culture during stat examination and to achieve a quick detection of central nervous system infections. Source

De Grazia S.,University of Palermo | Bonura F.,University of Palermo | Colomba C.,University of Palermo | Cascio A.,Messina University | And 5 more authors.
Infection, Genetics and Evolution

Uninterrupted surveillance conducted in Palermo, Sicily, for 27years (1985-2012) detected rotavirus infection in 32.7% of 6522 children <5years of age, hospitalised at the "G. Di Cristina" Children's Hospital of Palermo. Increased rotavirus activity usually occurred from the beginning of winter to mid-spring. G1P[8] rotaviruses were the prevalent strains in most of the years and were only occasionally overcome by G9P[8], G4P[8] or G2P[4]. The circulation of non-G1P[8] strains was discontinuous and fluctuating. Phylogenetic analyses revealed an heterogeneous population of viruses within each genotype, with different lineages and sublineages emerging over the time. Amino acid substitutions in both VP7 and VP8* antigenic epitopes were generally associated with different lineages/sublineages, emerging sequentially and replacing partially or completely the former strains. The present study summarises one of the longest surveillance activities conducted in the European continent, offering a useful temporal observatory of rotavirus epidemiology and strains variation and evolution in a settled population. © 2014 Elsevier B.V. Source

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