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Schlatter J.,Unit of Forensic Toxicology | Chiadmi F.,Unit of Forensic Toxicology | Gandon V.,Hopital Jean Verdier AP HP | Gandon V.,Center Hospitalier Sud Francilien | And 2 more authors.
Human and Experimental Toxicology

Background: Methanol, acetaldehyde, acetone, and ethanol, which are commonly used as biomarkers of several diseases, in acute intoxications, and forensic settings, can be detected and quantified in biological fluids. Gas chromatography (GC)-mass spectrometry techniques are complex, require highly trained personnel and expensive materials.Gas chromatographic determinations of ethanol,methanol, and acetone have been reported inone study with suboptimal accuracy.Our objective was to improve the assessment of these compounds in human blood using GCwith flame ionization detection.Methods: Anamount of 50 μl of blood was diluted with 300 μl of sterilewater, 40 μl of 10% sodium tungstate, and 20 μl of 1% sulphuric acid. After centrifugation, 1 μl of the supernatant was injected into the gas chromatograph.We used a dimethylpolysiloxane capillary column of 30m×0.25mm×0.25 mm. Results:We observed linear correlations from7.5 to 240mg/l formethanol, acetaldehyde, and acetone and from 75 to 2400mg/l for ethanol. Precision at concentrations 15, 60, and 120mg/l formethanol, acetaldehyde, and acetone and 150, 600, and 1200mg/ml for ethanol were 0.8-6.9%. Ranges of accuracy were 94.7-98.9% formethanol, 91.2-97.4% for acetaldehyde, 96.1-98.7% for acetone, and 105.5-111.6% for ethanol. Limits of detection were 0.80 mg/l for methanol, 0.61 mg/l for acetaldehyde, 0.58 mg/l for acetone, and 0.53 mg/l for ethanol. Conclusion: This method is suitable for routine clinical and forensic practices. © The Author(s) 2014. Source

Auvity S.,Unit of Pharmacy | Chiadmi F.,Unit of Forensic Toxicology | Cisternino S.,Unit of Pharmacy | Fontan J.-E.,Unit of Pharmacy | Schlatter J.,Unit of Forensic Toxicology
Analytical Chemistry Insights

A stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method was developed for the determination of betaxolol hydrochloride, a drug used in the treatment of hypertension and glaucoma. The desired chromatographic separation was achieved on a Nucleosil C18, 4 μm (150 × 4.6 mm) column, using isocratic elution at a 220 nm detector wavelength. The optimized mobile phase consisted of a 0.02 M potassium dihydrogen phosphate: methanol (40:60, v/v, pH 3.0 adjusted with o-phosphoric acid) as solvent. The flow rate was 1.6 mL/min and the retention time of betaxolol hydrochloride was 1.72 min. The linearity for betaxolol hydrochloride was in the range of 25 to 200 μg/mL. Recovery for betaxolol hydrochloride was calculated as 100.01%-101.35%. The stability-indicating capability was established by forced degradation experiments and the separation of unknown degradation products. The developed RP-HPLC method was validated according to the International Conference on Harmonization (ICH) guidelines. This validated method was applied for the estimation of betaxolol hydrochloride in commercially available tablets. © the author(s), publisher and licensee Libertas Academica Ltd. Source

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