Unit of Toxicology

Coimbatore, India

Unit of Toxicology

Coimbatore, India

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Deglon J.,Unit of Toxicology | Deglon J.,University of Geneva | Thomas A.,Unit of Toxicology | Thomas A.,University of Geneva | And 3 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

Because of the emergence of dried blood spots (DBS) as an attractive alternative to conventional venous plasma sampling in many pharmaceutical companies and clinical laboratories, different analytical approaches have been developed to enable automated handling of DBS samples without any pretreatment. Associated with selective and sensitive MS-MS detection, these procedures give good results in the rapid identification and quantification of drugs (generally less than 3 min total run time), which is desirable because of the high throughput requirements of analytical laboratories. The objective of this review is to describe the analytical concepts of current direct DBS techniques and to present their advantages and disadvantages, with particular focus on automation capacity and commercial availability. Finally, an overview of the different biomedical applications in which these concepts could be of major interest will be presented. [Figure not available: see fulltext.] © 2011 Springer-Verlag.


PubMed | Unit of Toxicology, Johns Hopkins University, University of Genoa, National Research Council Italy and 2 more.
Type: | Journal: International journal of cardiology | Year: 2016

Among endocannabinoid (EC)-related mediators, Oleoyl-ethanolamide (OEA) and Palmitoyl-ethanolamide (PEA), two endogenous PPAR agonists with lipolytic and anti-inflammatory action, respectively, are being actively investigated. Here, we assessed the potential association between plasma levels of PEA and OEA and coronary function in a cohort including normal, overweight, obese, and morbidly obese (MOB) individuals.Myocardial perfusion and endothelium-related myocardial blood flow (MBF) responses to cold pressor test (CPT) and during pharmacological vasodilation with dipyridamole were measured with Circulating levels of PEA and VCAM-1 were increased in MOB as compared to normal weight subjects. Circulating levels of OEA and PEA were associated with body mass index, but not with adhesion molecules. Increases of PEA levels were associated with and predictive of worsened coronary function in MOB and the overall cohort studied.Plasma levels of PEA are increased in MOB patients and associated with coronary dysfunction as a functional precursor of CAD process. Larger trials are needed to confirm PEA as a potential circulating biomarker of coronary dysfunction in both MOB patients and the general population.


Suvetha L.,Unit of Toxicology | Ramesh M.,Unit of Toxicology | Saravanan M.,Unit of Toxicology
Environmental Toxicology and Pharmacology | Year: 2010

The effects of acute and sublethal toxicity of cypermethrin, a synthetic pyrethroid insecticide on plasma electrolytes (Na+, K+ and Cl-) levels and gill Na+/K+-ATPase activity in a common carp Cyprinus carpio were examined. The 24 h LC50 value of cypermethrin for C. carpio was 1.86 ppm. During acute exposure, cypermethrin caused adverse effects in the form of hyponatreima, hypokalemia and hypochloremia and inhibition of gill Na+/K+-ATPase activity. In sublethal exposure to cypermethrin (0.186 ppm for 35 days), plasma Na+ was decreased throughout the exposure period except 7th day whereas plasma K+ level was increased up to 28th day, then declined. However, plasma Cl- level was decreased. Likewise, gill Na+/K+-ATPase activity was decreased as the exposure period extended. The present study indicates that cypermethrin was highly toxic to freshwater fish and ion levels in blood as measured by specific ion concentrations (Na+, K+ and Cl-) and changes in gill Na+/K+-ATPase activity may represent a sensitive and useful nonspecific biomarkers of chemical exposure and effects. © 2009 Elsevier B.V. All rights reserved.


PubMed | Unit of Toxicology
Type: Journal Article | Journal: Analytical and bioanalytical chemistry | Year: 2012

Because of the emergence of dried blood spots (DBS) as an attractive alternative to conventional venous plasma sampling in many pharmaceutical companies and clinical laboratories, different analytical approaches have been developed to enable automated handling of DBS samples without any pretreatment. Associated with selective and sensitive MS-MS detection, these procedures give good results in the rapid identification and quantification of drugs (generally less than 3 min total run time), which is desirable because of the high throughput requirements of analytical laboratories. The objective of this review is to describe the analytical concepts of current direct DBS techniques and to present their advantages and disadvantages, with particular focus on automation capacity and commercial availability. Finally, an overview of the different biomedical applications in which these concepts could be of major interest will be presented.

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