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Klippert A.,Unit of Infection Models German Primate Center Goettingen Germany | Stolte-Leeb N.,Unit of Infection Models German Primate Center Goettingen Germany | Neumann B.,Unit of Infection Models German Primate Center Goettingen Germany | Sauermann U.,Unit of Infection Models German Primate Center Goettingen Germany | And 4 more authors.
Journal of Medical Primatology | Year: 2015

Background: T-follicular helper (TFH) cells are an important population in lymph nodes (LNs) contributing to the generation of highly specific B cells. For SIV studies in rhesus macaques (RM), analysis of LN is necessary, but restricted due to invasive sampling. We applied the minimally invasive LN fine-needle aspiration (LN-FNA) and examined dynamics of TFH cells during SIV infection. Materials and methods: LN-FNA and LN resection were carried out on uninfected RM. Lymphocytes were analyzed by flow cytometry. Additionally, cells obtained by LN-FNA over time from SIV-infected RM were analyzed. Results: Percentages of lymphocyte subsets were similar in LN aspirates and whole LNs. Analysis of LN aspirates from SIV-infected RM demonstrated a decrease of CD4+ T cells, while TFH cell frequencies increased over time and correlated significantly with plasma viral load. Conclusions: By applying LN-FNA, we showed that TFH cell expansion in chronic SIV infection is associated with viral load. © 2015 John Wiley & Sons A/S. Source

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