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Di Guglielmo C.,Unit of Experimental Toxicology and Ecotoxicology | De Lapuente J.,Unit of Experimental Toxicology and Ecotoxicology | Porredon C.,Unit of Experimental Toxicology and Ecotoxicology | Ramos-Lopez D.,Unit of Experimental Toxicology and Ecotoxicology | And 2 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2012

Safety and toxic effects of nanoparticles are still largely unexplored due to the multiple aspects that influence their behaviour toward biological systems. Here, we focus the attention on 12 nm spherical gold nanoparticle coated or not with hyaluronic acid compared to its precursor counterpart salt. Results ranging from the effects of a 10-days exposure in an in vitro model with BALB/c 3T3 fibroblast cells show how 12 nm spherical gold nanoparticles are internalized from 3T3 cells by endo-lysosomal pathway by an indirect measurement technique; and how gold nanoparticles, though not being a severe cytotoxicant, induce DNA damage probably through an indirect mechanism due to oxidative stress. While coating them with hyaluronic acid reduces gold nanoparticles cytotoxicity and slows their cell internalization. These results will be of great interest to medicine, since they indicate that gold nanoparticles (with or without coating) are suitable for therapeutic applications due to their tunable cell uptake and low toxicity. Copyright © 2012 American Scientific Publishers.

De Marzi L.,University of LAquila | Ottaviano L.,University of LAquila | Perrozzi F.,University of LAquila | Nardone M.,University of LAquila | And 6 more authors.
Journal of Biological Regulators and Homeostatic Agents | Year: 2014

This study was carried out by varying both graphene oxide (GO) concentration (10 μg/mL, 50 μg/mL, 100 μg/mL) and flakes sizes of 1320 nm and 130 nm. Characterization by scanning electron microscopy and Raman spectroscopy demonstrate that the area of GO flakes varies of one order of magnitude but their chemical structure remains unmodified. A 24-h cytotoxicity test showed, for A549, a loss in the viability, while the test exhibits overall a positive increase in the viability for CaCo2 and Vero. A 24-h comet assay shows a marked GO genotoxicity: for micrometer-sized GO flakes the genotoxicity is in positive correlation with the concentration, while for nanometer-sized GO flakes there was a high degree of genotoxicity at the lowest concentration tested. Copyright © by BIOLIFE, s.a.s.

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