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Xie W.,Union Hospital Affiliated to Tongji Medical College
Zhongguo yi liao qi xie za zhi = Chinese journal of medical instrumentation | Year: 2010

Traditionally, a novice ear doctor acquires the skillfully operative ability through dissecting cadaveric temporal bone in ear surgical training lab. For conceivable reasons donated bodies are always short in supply, virtual reality-based simulator provides a new model for surgical training. It has been proven that the simulator is a useful tool for surgical training. And it can be used in the rehearsal of operation procedures, real-time operation guidance, and remote surgery. Our design is different from the existing virtual surgical system. It has a parallel, distributed multi-workstation with the vivid three-dimensional model of temporal bone, and it can be used not only to learn the ear surgical skills in the virtual environment, but also preoperative plan and surgical consultation. Source

Zhang G.,Union Hospital Affiliated to Tongji Medical College | Lan X.,Union Hospital Affiliated to Tongji Medical College | Chen Q.,Union Hospital Affiliated to Tongji Medical College | Cao W.,Union Hospital Affiliated to Tongji Medical College | And 2 more authors.
He Jishu/Nuclear Techniques | Year: 2010

A recombinant adenovirus Ad5-sr39tk-IRES-VEGF165(Ad5-SIV)was constructed in our lab, using mutant simplex herpes viral thymidine kinase reporter gene(HSV1-sr39tk)as a report gene, and human vascular endothelial growth factor 165(VEGF165)as a therapeutic gene. A replication-defective adenovirus carrying the enhanced green fluorescent protein gene(Ad5-EGFP)was used as a control. MSCs were infected with Ad5-SIV at different multiplicity of infection(MOI = 0, 10, 25, 50, 75 and 100). The expression of HSV1-sr39tk and VEGF165 was detected by immunofluorescence, and the secretion of VEGF was detected by enzyme-linked immunosorbent assay(ELISA). The cellular uptake of 131I-FIAU was performed to detect the expression of HSV1-sr39tk. The immunofluorescence showed HSV1-sr39tk and VEGF165 protein could express successfully at Ad5-SIV-infected MSCs. The cellular uptake of 131I-FIAU increased with the virus titer(P<0.05). The time dependent uptake showed uptake rates increased rapidly from 30 min to 150 min, with a plateau after 150 min. ELISA results showed VEGF165 protein secretion increased with the titer(P<0.05). The VEGF165 protein secretion correlated highly with the uptake rate of 131I-FIAU(P<0.05). In conclusion, HSV1-sr39tk and VEGF165 could express successfully after MSCs were infected with Ad5-SIV. The expression of therapy gene correlated significantly with the reporter gene. This provides a theoretical basis for in vitro nuclear reporter gene imaging with this system. Source

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