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Östermalm, Sweden

Guerin P.,VetAgro Sup | Menezo Y.,Unilabs | Menezo Y.,Laboratoire dEylau
Zygote | Year: 2011

The culture of early preimplantation stage embryo is still delicate and the metabolic pathways of embryos are not completely understood. Embryo needs are evolutionary during the preimplantation development, consequently it is difficult to meet embryo needs in vitro. Culture conditions have to respect several physical and chemical equilibria: such as redox potential, pH, osmotic pressure, metabolic flux of energetic compounds, endogenous pools of amino acids and transcripts, etc. Embryo culture media are generally supplemented with amino acids, glucose, other energetic metabolites and antioxidant compounds, vitamin, and growth factors etc. Furthermore autocrine and paracrine regulation of embryo development probably exist. In fact embryo culture conditions have to be as non-toxic as possible. Various types of co-culture systems have been devised to overcome these problems. Complex interrelations exist between embryos and co-cultured cells. The beneficial effects of co-cultured cells may be due to continuous modifications of the culture medium, i.e. the elimination of toxic compounds and/or the supply of embryotrophic factors. Copyright © Cambridge University Press 2010.

Herrlin S.V.,Herrlin Invest | Wange P.O.,Karolinska Institutet | Lapidus G.,Unilabs | Hallander M.,Karolinska Institutet | And 3 more authors.
Knee Surgery, Sports Traumatology, Arthroscopy | Year: 2013

Purpose: The aim of this prospective randomized intervention study was to evaluate the outcome at a 2 and 5 year follow-up whether combined arthroscopic surgery followed by exercise therapy was superior to the same exercise therapy alone when treating non-traumatic, degenerative medial meniscal tears. Methods: Ninety-six middle-aged patients with MRI-verified degenerative medial meniscus tear and radiographic osteoarthritis grade ≤1 (Ahlbäck) participated in the study. Radiographic examination was done before randomization and after 5 years. The patients were randomly assigned to either arthroscopic treatment followed by exercise therapy for 2 months or to the same exercise therapy alone. At the start of the study and at the follow-ups at 24 and 60 months the patients answered three questionnaires KOOS, Lysholm Knee Scoring Scale and Tegner Activity Scale and made pain ratings on the Visual Analogue Scale (VAS). Results: Both groups showed highly significant clinical improvements from baseline to the follow-ups at 24 and 60 months on all subscales of KOOS, Lysholm Knee Scoring Scale and VAS (p < 0. 0001). No group differences were found at any of the testing occasions. One third of the patients that were treated with exercise therapy alone did not feel better after the treatment but were improved after arthroscopic surgery. According to radiographic findings two patients from each group had a slight progression of their osteoarthritis after 5 years. Conclusion: The findings indicate that arthroscopic surgery followed by exercise therapy was not superior to the same exercise therapy alone for this type of patients. Consequently, exercise therapy can be recommended as initial treatment. However, one third of the patients from the exercise group still had disabling knee symptoms after exercise therapy but improved to the same level as the rest of the patients after arthroscopic surgery with partial meniscectomy. Level of evidence: I. © 2012 Springer-Verlag.

Korte W.,Institute For Klinische Chemie Und Hamatologie | Korte W.,University of Bern | Engler H.,Institute For Klinische Chemie Und Hamatologie | Riesen W.F.,Institute For Klinische Chemie Und Hamatologie | Brinkmann T.,Unilabs
Clinical Laboratory | Year: 2012

Background: Different FT3 and FT4 assays report significantly different results. We compared the distribution of FT3 and FT4 in a cohort of Swiss patients measured with DxI 800, AxSYM, and Immulite 2000. Methods: TSH, FT3, and FT4 values were measured in 1,938 serum samples. Patients were classified on the basis of their TSH values as low, normal, and high. For each class of TSH values, concordances of FT3 and FT4 results were determined among the three assays. Results: For low TSH values in all three assays FT3 (FT4) concordance of DxI - AxSYM, DxI - Immulite, and AxSYM - Immulite was determined as 83.1%, 76.2% 68.5% (60.8%, 74.6%, 83.1%), for normal TSH as 89.2%, 79.0%, 75.3% (83.9%, 85.5%, 83.1%) and for elevated TSH as 78.0%, 86.0%, 78.0% (84.0%, 90.0%, 90.0%), respectively. Low FT4 concordance rates with DxI 800 were mainly caused by its FT4 upper reference limit of 14.1 pmol/L. Using a cut-off of 16.1 pmol/L concordances with AxSYM and Immulite were improved to 77.7% and 86.9% (low TSH), 92.5% and 96.2% (normal TSH), and 90.0% and 92.2% (high TSH). Low FT3 concordance rates with Immulite were caused by its low FT3 upper reference limit of 6.29 pmol/L as 11.6% of patient samples with normal TSH value showed unusually elevated FT3 results. Conclusions: We showed an overall good concordance of FT3 and FT4 results, when stratified according to corresponding TSH values and the appropriate reference range is used. However, our data also show that problems of interpretation of results based on numerical values have yet not been solved.

Olausson J.,Entrance | Petersonl C.,Entrance | Bergstrom M.,Unilabs | Larsson A.,Entrance
Clinical Laboratory | Year: 2015

Background: BNP and NT-proBNP are widely used as rule-out tests for heart failure (HF) and they are frequently requested by primary care doctors. A point-of-care (POC) test would reduce the time to diagnosis for patients with suspected HF. The aim of the study was to evaluate a POC BNP test. Methods: Plasma BNP results obtained with the Meritas® POC instrument (n = 82) were compared with the corresponding plasma BNP results analyzed on an Advia Centaur analyzer (Siemens Healthcare, Erlangen, Germany)- Results: The two methods showed concordant results with a Passing-Bablok correlation between the two methods: BNPMeritas = 1.00 x BNPSiemens + 1.09; r = 0.9773. Conclusions: The study show that the Meritas® BNP assay could be used in primary care permitting rapid BNP testing to rule out heart failure. © since 2008 All rights reserved. Copyright Clinical Laboratory Publications GmbH

Beris P.,University of Geneva | Georgiou G.,Unilabs
Seminars in Hematology | Year: 2012

Myelodysplastic syndromes (MDS) were first presented as a separate entity in hematology in 1982 by the French-American-British (FAB) group. Although widely accepted, this classification was revised in 2001 and again in 2008 by the World Health Organization (WHO). During that time, a great effort was made by diagnostic entities of world renown as well as by other research groups and individuals to establish a reliable prognostic system. Today, two systems are accepted by the hematologic community: the International World Prognostic Scoring System (IPSS) and the WHO Prognostic Scoring System (WPSS). Almost from the beginning, it was assumed that MDS are clonal disorders. Cytogenetics not only proved the clonality in the majority of cases but also greatly contributed to the stratification of patients into risk categories. With the progress made in treatment for MDS, comorbidities became an important part of the integral pretreatment consideration, as patients with a high index of comorbidity evaluation very often do not benefit from treatment. Recent advances in the diagnostic and prognostic evaluation of MDS patients were achieved by studying the effect of age in different MDS subgroups and the role of molecular markers in predicting clinical evolution. © 2012 Elsevier Inc.

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