Marchesi L.F.Q.P.,Federal University of São Carlos |
Simoes F.R.,UNIFESP |
Pocrifka L.A.,Federal University of São Carlos |
Pereira E.C.,Federal University of São Carlos
Journal of Physical Chemistry B | Year: 2011
In this paper, the electrochemical degradation of polypyrrole film was studied by means of overpotential application. The overpotential was 0.58 V versus SCE, and after every 5 min of application of 0.58 V, a cyclic voltammogram was recorded in the range of -0.7 to 0.5 V as well as an electrochemical impedance spectroscopy and electrochemical quartz crystal microbalance (EIS and EQCM). The main characteristic is the huge increase in the charge transfer resistance (rct), which indicates that the insertion process of ions in the polymer matrix is hindered by the electrochemical degradation. Once the process of insertion is damaged, the number of intercalated ions in the matrix should decrease, which is expressed by the low-frequency capacitance, which is proportional to the number of intercalated ions in the polymeric matrix. The decrease of intercalated ions has an influence in the mass variation of the polymer film, which is confirmed by EQCM measurements. © 2011 American Chemical Society.
de Franca N.R.,UNIFESP
Revista brasileira de reumatologia | Year: 2010
RNA interference (RNAi) is a post-transcriptional gene silencing mechanism preserved during evolution. This mechanism, recently described, is mediated by small double-stranded RNAs (dsRNAs) that can specifically recognize a target mRNA sequence and mediate its cleavage or translational repression. The use of RNAi as a tool for gene therapy has been extensively studied, especially in viral infections, cancer, inherited genetic disorders, cardiovascular and rheumatic diseases. Together with data from human genome, the knowledge of gene silencing mediated by RNAi could allow a functional determination of virtually any cell expressed gene and its involvement in cellular functioning and homeostasis. Several in vitro and in vivo therapeutic studies with autoimmune disease animal models have been carried out with promising results. The pathways of tolerance breakage and inflammation are potential targets for RNAi therapy in inflammatory autoimmune diseases. This review will present the basic principles of RNAi and discuss several aspects of RNAi-based therapeutic approaches, from in vitro tool design and target identification to in vivo pre-clinical drug delivery, and tests of autoimmune diseases in human cells and animal models. Finally, this review will present some recent clinical experience with RNAi-based therapy.
Kayo A.H.,Federal University of São Paulo |
Peccin M.S.,UNIFESP |
Sanches C.M.,Federal University of São Paulo |
Trevisani V.F.M.,University of Santo Amaro |
Trevisani V.F.M.,University of Sao Paulo
Rheumatology International | Year: 2012
The purpose of this study was to evaluate and compare the effectiveness of muscle-strengthening exercises (MS) and a walking program (WA) in reducing pain in patients with fibromyalgia. Ninety women, 30-55 years of age, diagnosed with fibromyalgia according to the American College of Rheumatology 1990 criteria, were randomized into 3 groups: WA Group, MS Group, and control group. Pain (visual analog scale) was evaluated as the primary outcome. Physical functioning (Fibromyalgia Impact Questionnaire, FIQ), health-related quality of life (Short-Form 36 Health Survey, SF-36), and use of medication were evaluated as secondary outcomes. Assessments were performed at baseline, 8, 16, and 28 weeks. Intention-to-treat and efficacy analyses were conducted. Sixty-eight patients completed the treatment protocol. All 3 groups showed improvement after the 16-week treatment compared to baseline. At the 28-week follow-up, pain reduction was similar for the WA and MS groups (P = 0.39), but different from the control group (P = 0.01). At the end of the treatment, 80% of subjects in the control group took pain medication, but only 46.7% in the WA and 41.4% in the MS groups. Mean FIQ total scores were lower for the WA and MS groups (P = 0.96) compared with the control group (P < 0.01). Patients in the WA and MS groups reported higher scores (better health status) than controls in almost all SF-36 subscales. MS was as effective as WA in reducing pain regarding all study variables; however, symptoms management during the follow-up period was more efficient in the WA group. © Springer-Verlag 2011.
De Carvalho F.,Federal University of São Paulo |
Vettore A.L.,UNIFESP |
Colleoni G.W.B.,Federal University of São Paulo
Clinical and Developmental Immunology | Year: 2012
Cancer/Testis Antigens (CTAs) are a promising class of tumor antigens that have a limited expression in somatic tissues (testis, ovary, fetal, and placental cells). Aberrant expression of CTAs in cancer cells may lead to abnormal chromosome segregation and aneuploidy. CTAs are regulated by epigenetic mechanisms (DNA methylation and acetylation of histones) and are attractive targets for immunotherapy in cancer because the gonads are immune privileged organs and anti-CTA immune response can be tumor-specific. Multiple myeloma (MM) is an incurable hematological malignancy, and several CTAs have been detected in many MM cell lines and patients. Among CTAs expressed in MM we must highlight the MAGE-C1/CT7 located on the X chromosome and expressed specificity in the malignant plasma cells. MAGE-C1/CT7 seems to be related to disease progression and functional studies suggests that this CTA might play a role in cell cycle and mainly in survival of malignant plasma cells, protecting myeloma cells against spontaneous as well as drug-induced apoptosis. © Copyright 2012 Fabricio de Carvalho et al.
Estadella D.,Federal University of São Paulo |
Da Penha Oller Do Nascimento C.M.,Federal University of São Paulo |
Oyama L.M.,Federal University of São Paulo |
Ribeiro E.B.,Federal University of São Paulo |
And 2 more authors.
Mediators of Inflammation | Year: 2013
The ingestion of excessive amounts of saturated fatty acids (SFAs) and transfatty acids (TFAs) is considered to be a risk factor for cardiovascular diseases, insulin resistance, dyslipidemia, and obesity. The focus of this paper was to elucidate the influence of dietary SFA and TFA intake on the promotion of lipotoxicity to the liver and cardiovascular, endothelial, and gut microbiota systems, as well as on insulin resistance and endoplasmic reticulum stress. The saturated and transfatty acids favor a proinflammatory state leading to insulin resistance. These fatty acids can be involved in several inflammatory pathways, contributing to disease progression in chronic inflammation, autoimmunity, allergy, cancer, atherosclerosis, hypertension, and heart hypertrophy as well as other metabolic and degenerative diseases. As a consequence, lipotoxicity may occur in several target organs by direct effects, represented by inflammation pathways, and through indirect effects, including an important alteration in the gut microbiota associated with endotoxemia. Interactions between these pathways may perpetuate a feedback process that exacerbates an inflammatory state. The importance of lifestyle modification, including an improved diet, is recommended as a strategy for treatment of these diseases. © 2013 Débora Estadella et al.
Caixeta G.C.S.,Bandeirante University of Sao Paulo |
Dona F.,Bandeirante University of Sao Paulo |
Brazilian Journal of Otorhinolaryngology | Year: 2012
Abnormal body balance and cognitive dysfunction may develop in elderly patients with chronic vestibular dysfunction. Aim: To evaluate the relationship between cognitive processing and body balance in elderly patients with chronic peripheral vestibular disease. Type of Study: Cross-sectional. Methods: Seventy-six patients (≥ 60 years) with chronic peripheral vestibular dysfunction and dizziness for more than three months were enrolled. The tests for investigating body balance were: the Berg Balance Scale (BBS), Dynamic Gait Index (DGI), Timed Up and Go Test (TUGT) Timed Up and Go Test modified (TUGTm); the Mini Mental State Examination (MMSE), Test Clock (RT,) and Verbal Fluency Test (VF) were applied for assessing cognition. Results: The mean age was 69.03 years (SD=6.21 years); most were female (82.9%). There was a significant negative correlation between the MMSE and the TUGT (ρ=-0.312; p=0.01), MMSE and TUGTm (ρ=-0.306; p=0.01), FV and TUGT (ρ=-0.346; p=0.01), and FV and TUGTm (ρ=-0.536; p=0.01); there was a significant positive correlation between the TR and BBS (ρ=0.343; p=0.01), TR and DGI (ρ=0.298; p=0.01), FV and BBS (ρ=0.299; p=0.01), and FV and DGI (ρ=0.306; p=0.01). Conclusion: Elderly patients with chronic peripheral vestibular disease and worse performance in body balance tests have functional impairment in cognitive skills.
Vellozo E.P.,Federal University of São Paulo |
Revista Brasileira de Hematologia e Hemoterapia | Year: 2010
This article focuses on the contribution of fortified foods, developed from growing scientific knowledge, whose functions go beyond nutrition. Its main goal is to report on the physiological effects that improve health and wellness by correcting deficiencies inherent from the economic and social status of patients, pursuing practical conditions and respecting regional habits, in order to meet the needs of adequate micronutrient intake, in particular for locations where prevalence is high. Fortification should encompass all population segments, mainly in the pediatric phase, when needs are relatively high due to growth. The World Bank refers to the importance of food fortification as a strategy to fight against micronutrient deficiency throughout the world. It also claims that "no other technology can offer an opportunity to improve lives at such a low cost and in such a short period". Studies have shown that food fortification is one of the best and most effective processes to prevent iron deficiency in the population. Therefore, in developing countries, such as Brazil, fortified foods are imperative to improve and even to cure iron deficiency anemia.
Barros R.C.,University of Sao Paulo |
Basgalupp M.P.,UNIFESP |
De Carvalho A.C.P.L.F.,University of Sao Paulo |
Freitas A.A.,University of Kent
Genetic and Evolutionary Computation Conference, GECCO'11 - Companion Publication | Year: 2011
Decision tree induction is one of the most employed methods to extract knowledge from data, since the representation of knowledge is very intuitive and easily understandable by humans. The most successful strategy for inducing decision trees, the greedy top-down approach, has been continuously improved by researchers over the years. This work, following recent breakthroughs in the automatic design of machine learning algorithms, proposes two different approaches for automatically generating generic decision tree induction algorithms. Both approaches are based on the evolutionary algorithms paradigm, which improves solutions based on metaphors of biological processes. We also propose guidelines to design interesting fitness functions for these evolutionary algorithms, which take into account the requirements and needs of the end-user. © 2011 ACM.
Kraychete D.C.,Federal University of Bahia |
Revista Brasileira de Anestesiologia | Year: 2011
Background and objectives: Painful neuropathies are common and often difficult to treat. The objective of this report was to review the subject to facilitate diagnosis and pain relief. Contents: The classification, causes, type of fibers involved, manifestations, diagnosis, adjuvant tests, questionnaires used in the diagnosis, and treatment are described. Conclusions: The subject is very broad and involves several causes and treatments that often should be combined to obtain adequate pain control. © 2011 Elsevier Editora Ltda.
Alencar de Castro R.J.,Federal University of São Paulo |
Leal P.C.,Unifesp |
Revista Brasileira de Anestesiologia | Year: 2013
Background and objectives: Despite advances, inappropriate analgesic treatment for burn patients is still seen. The objective of this review was to collect data on pain management in burn patients. Content: We reviewed the mechanisms of pain, burn patient assessment, as well as pharmacological and non-pharmacological treatment. Conclusion: Pain management in burn patients is still a challenge for the multidisciplinary team. Frequent and continuous evaluation of the patient's response is very important due to the various stages that the hospitalized burn patient goes through, as well as a combination therapy with analgesic and non-pharmacological measures. Understanding the complexity of the pathophysiological, psychological, and biochemical changes a burn patient presents is the first step to achieve success in analgesic management. Justifi cativa e objetivos: Apesar dos avanços, ainda é observado manejo analgésico inadequado dos pacientes com queimaduras. O objetivo desta revisão foi coletar dados sobre tratamento da dor em queimados. Conteúdo: Foi feita revisão sobre os mecanismos de dor, avaliação do paciente com queimadura e o tratamento farmacológico e não farmacológico. Conclusões: O manejo da dor em pacientes vítimas de queimaduras ainda é um desafi o por parte da equipe multiprofi ssional. A avaliação frequente e contínua da resposta apresentada pelo paciente é muito importante, tendo em vista os vários momentos por que passa o paciente internado em decorrência de uma queimadura, além de uma terapêutica combinada com medicações analgésicas e medidas não farmacológicas. Entender a complexidade de alterações fi siopatológicas, psicológicas e bioquímicas apresentadas por um paciente em tratamento de queimadura é o primeiro passo para alcançar o sucesso no seu manejo analgésico. © 2013 Elsevier Editora Ltda..