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Campo Grande, Brazil

Rodrigues A.P.D.C.,Anhanguera-Uniderp University | Laura V.A.,Embrapa Gado de Corte | Pereira S.R.,Federal University of Sao Carlos | Ferreira E.,Uniderp | de Freitas M.E.,Federal University of Grande Dourados
Ciencia Rural | Year: 2011

The emergence of parsley seedlings in the field is heterogeneous and requires a relatively long period of time, which can take more than four weeks, depending on the temperature and soil moisture, justifying the use of techniques to increase and standardize germination process. This research aimed to evaluate the storage potential parsley primed seeds. The experiment was carried out in a completely randomized block with four repetitions of 100 seeds. It was used primedseeds primed soaked in a polyethylene glycol 6000 solution at -1.5MPa and unprimed seeds. Emergence, speed of index emergence, speed of emergence and number of established seedlings were evaluated 30 days after sowing. Seeds of parsley, primed or unprimed, lost their viability after 90 days of storage at the conditions tested.

De Carvalho P.D.T.C.,Federal University of Sergipe | Da Silva I.S.,Federal University of Mato Grosso | Dos Reis F.A.,Federal University of Mato Grosso | Perreira D.M.,Uniderp | Aydos R.D.,Federal University of Mato Grosso
Acta Cirurgica Brasileira | Year: 2010

Purpose: To determine the influence of low-power laser (660 nm) on the collagen percentage and macrophages in skin wounds in diabetic rats. Methods: 30 male Wistar rats were used, distributed in two groups: laser treated diabetic (n= 15); untreated diabetic (n = 15). The diabetes was induced by intravenous injection of alloxan into the dorsal vein of the penis, at a rate of 0.1 ml of solution per 100 g of body weight. A wound was made on the back of all the animals. Groups 2 were treated with Aluminium Gallium Indium Phosphide - InGaAlP type diode laser (Photon Laser III DMC®) with a continuous output power of 100 mW and wavelength (λ) of 660 nm (4 J/cm2) for 24 s. five animal from each group was sacrificed on the 3rd, 7th and 14th days after wounding. Samples were taken, embedded in paraffin, stained with hematoxylin-eosin, Masson's trichrome, and immunohistochemical macrophage. morphometrically analyzed using the Image Pro Plus 4.5 software. The percentages of collagen fibers and macrophages were determined from the samples from the euthanasia animals. Results: The data were treated statistically using analysis of variance (ANOVA) and the Post-hocTukey test. The significance level was set at 0.05 or 5%.Conclusion: The low-power laser (660 nm) was shown to be capable of influencing the collagen percentage in skin wounds by increasing the mean quantity of collagen fibers and macrophages.

Silva D.N.,University for the State and Pantanal Region Development | Coelho J.,Uniderp | Frazilio F.D.O.,Uniderp | Odashiro A.N.,University for the State and Pantanal Region Development | And 5 more authors.
Acta Cirurgica Brasileira | Year: 2010

Purpose: To evaluate axonal regeneration after end-to-side nerve repair with fibrin glue in rats. Methods: Forty-five Wistar rats were divided into three groups: group A (n=15), were not submitted to surgery (control group); group B (n=15) were submitted to fibular transection without repair; and group C (n=15), were submitted to fibular transection with end-to-side nerve anastomosis using fibrin glue, in the lateral surface of an intact tibial nerve. The three groups were submitted to walking track (30 and 90 days) and posterior morphometrical analysis (90 days). Results: The functional tests demonstrated that there was no difference in the walking track during the study in group A (p>0.05). The group B had walking pattern impairment in the two tests (p>0.05). The group C had walking pattern impairment in the first test, with important recovery in the second test (p<0.05). The morphometrical assessment revealed significantly higher number of regenerated mielinates axons in group C, compared to group B (p<0.05). Conclusion: The end-to-side nerve repair with fibrin glue shows axonal recovery, demonstrated through functional and morphometrical ways in rats.

da Silva A.P.M.,Curso de Administracao de Empresas | Bono J.A.M.,Uniderp | Pereira F.A.R.,Uniderp
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2014

The ethanol industry in Brazil is booming, resulting in the production of byproducts, among them the vinasse. The industry have used vinasse in agricultural areas through fertigation. The main objective of this study was to analyse the effect of application of vinasse in some chemical attributes of soil and productivity of sugarcane, for a period of three years. The experiment was conducted in soil classified as Quartzipsamment located in Campo Grande, MS, in the period from November 2008 to September 2011. The experimental plots were of 4.2 x 10.0 m (42 m2), and the treatments consisted of vinasse doses (0, 100, 200, 400 and 800 m3 ha-1), distributed in the field in random blocks design with four replications. Vinasse was retrieved from alcohol plant in the region and applied to seedlings of sugarcane (1st, 2nd and 3rd ratoon). The results showed that the vimasse used provided an increase in productivity of sugarcane and increases in pH and exchangeable potassium of soil levels, up to 0.4 m depth.

Matias R.,Anhanguera-Uniderp University | Roel A.R.,Catholic University Dom Bosco | Andrade Filho N.N.,Catholic University Dom Bosco | Schleder E.E.J.D.,Uniderp | And 2 more authors.
Bioscience Journal | Year: 2013

Bemisia tuberculata (Bondar, 1923) is the most important pest of cassava crops. This work describes the effect of different concentrations of CHCl3 extract from the leaves of Anacardium humile in relation to Bemisia tuberculata, under laboratory conditions, in cassava plants and chemical composition of this extract. Fractionation of the CHCl3 extract yielded the triterpene acids: oleanolic, ursolic and betulinic. Their structures were determined on the basis of spectral data (NMR-1D and 2D) and subsequent comparisons with the literature. Anacardic acid was identified in CHCl3 extract by high performance liquid chromatography (HPLC). CHCl3 extract caused mortality of whitefly nymphs of B. tuberculata from 66.30 to 74.90%, in doses tested. The insecticidal activity of CHCl3 extract of A. humile on the nymphs of the whitefly suggests that pentacyclic triterpenes and anacardic acid have potential insecticidal activity.

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