Pre-germination treatments and storage of cassava seeds and their correlation with emergence of seedlings [Tratamentos pré-germinativos e armazenamento de sementes de mandioca e sua correlação com a emergência de plântulas]
Mezzalira I.,Embrapa Cerrados |
Costa C.J.,Embrapa Clima Temperado |
Vieira E.A.,Embrapa Cerrados |
de Freitas J.F.,Embrapa Cerrados |
And 3 more authors.
Journal of Seed Science | Year: 2013
Despite propagation of the cassava crop be done by cuttings for commercial purposes, the majority of accesses keeps active the sexual propagation system; what is essential to genetic breeding programs. Cassava seeds, however, have low and uneven germination rates. Considering such event, the objective of this study was to evaluate the efficiency of the storage, and different pre-germination treatments, on cassava seedling emergence. For this, an experiment was carried out with seeds obtained by open pollination between plants of four sweet cassava cultivars, with 10 pre-germination treatments; in addition to a control treatment. The viability of seeds subjected to different treatments was assessed by tetrazolium test; and the assessment of seedling emergence was performed by daily counts of the number of emerged seedlings. Data obtained were expressed in: emergence percentage; emergence speed index; and mean time of seedling emergence. It was concluded that storage, at 4 °C, during one year, increases seed emergence percentage and favors cassava seedling emergence speed; and that the dry heat (60°C), during seven and 14 days, also favors their emergence speed.
De Vargas A.C.,Federal University of Santa Maria |
Monego F.,Contestado University |
Gressler L.T.,Federal University of Santa Maria |
De Avila Botton S.,Federal University of Santa Maria |
And 6 more authors.
BMC Research Notes | Year: 2013
Background: Rhodococcus equi is associated with pyogranulomatous infections, especially in foals, and this bacterium has also emerged as a pathogen for humans, particularly immunocompromised patients. R. equi infections in pigs, wild boar (Sus scrofa) and humans are mainly due to strains carrying the intermediate virulence (VapB) plasmid. In Brazil, R. equi carrying the VapB type 8 plasmid is the most common type recovered from humans co-infected with the human immunodeficiency virus (HIV). R. equi infection in pigs and wild boar is restricted predominantly to the lymphatic system, without any reports of pulmonary manifestations. Findings. This report describes the microbiological and histopathological findings, and molecular characterization of R. equi in two bronchopneumonia cases in wild boar using PCR and plasmid profile analysis by digestion with restriction endonucleases. The histological findings were suggestive of pyogranulomatous infection, and the plasmid profile of both R. equi isolates enabled the characterization of the strains as VapB type 8. Conclusions: This is the first report of bronchopneumonia in wild boar due to R. equi. The detection of the VapB type 8 plasmid in R. equi isolates emphasize that wild boar may be a potential source of pathogenic R. equi strains for humans. © 2013 de Vargas et al.; licensee BioMed Central Ltd.
Interleukin - 1β production and severity of mastitis post-inoculation of staphylococcus aureus in the mammary gland of bovines and bubalines [Produção de interleucina-1beta e severidade da mastite pós-inoculação de staphylococcus aureus na glândula mamária de bovinos e bubalinos]
Lazzari A.M.,Uniao Pioneira de Integracao Social UPIS |
de Oliveira M.V.S.,Uniao Pioneira de Integracao Social UPIS |
Moreti B.,Uniao Pioneira de Integracao Social UPIS |
Guimaraes G.,Uniao Pioneira de Integracao Social UPIS |
And 6 more authors.
Ciencia Rural | Year: 2014
This study aimed to analyze in cows and buffaloes, submitted to mastitis induced by inoculation of S. aureus, the concentration of the pro-inflammatory cytokine interleukin-1beta (IL-1β), the somatic cell count (SCC), and their correlation with some parameters of local and systemic response to inflammation. The animals had one mammary gland inoculated and the inflammatory process was monitored by milk culture, SCC, IL-1β measurement in the milk, evaluation of the gland appearance/consistency, milk secretion appearance (localized response to inflammation) and rectal temperature measurement (systemic response to inflammation). There was increase in the levels of IL-1β, SCC, and both local and systemic inflammatory response, in bovine and bubaline species. The production kinetics of the cytokine was different between the two species (P<0.05). Buffaloes showed a faster increase but achieved lower levels of interleukin-1beta, when compared to cows. Both species reached similar maximum counts (P>0.05) of SC/milk mL, with different concentrations (P<0.05) of IL-1β/mL. The parameters used to verify the local response to inflammation showed higher mean scores in bovine specie. Positive correlation between IL-1β concentration in the milk, SCC and parameters used to analyze the severity of mastitis was verified only in the bovine specie. The results evidenced that the kinetics of IL-1β production was different in the bovine and bubaline species, and demonstrated that the buffaloes developed a milder inflammatory process with faster recovery of the parameters used for mastitis severity evaluation. © 2014 Universidade Federal de Santa Maria. All rights reserved.