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Poznań, Poland

Celka Zbigniew Z.,Umultowska | Buczkowska K.,Adam Mickiewicz University | Baczkiewicz A.,Adam Mickiewicz University | Drapikowska M.,University of Life Sciences in Poznan
Acta Biologica Cracoviensia Series Botanica

We estimated similarity and differences at the level of isozymes among populations of Malva alcea from various habitats and parts of Central Europe. Our analyses revealed the activity of 8 enzyme systems: 6 polymorphic (PGI, IDH, PX, DIA, PGM, SHD KB) and 2 monomorphic (GOT, ME). The mean number of alleles per locus is 2.12. Nei's genetic distances among populations are small, not exceeding 0.3. Mean values of Nei's genetic similarity are typical of populations of the same species. Grouping by UPGMA based on Nei's genetic distances showed that the distinguished groups are only partly correlated with geographic region. © Polish Academy of Sciences and Jagiellonian University, Cracow 2010. Source

Slocinska M.,Umultowska | Antos-Krzeminska N.,Umultowska | Rosinski G.,Umultowska | Jarmuszkiewicz W.,Umultowska
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Uncoupling protein 4 (UCP4) is a member of the UCP subfamily that mediates mitochondrial uncoupling, and sequence alignment predicts the existence of UCP4 in several insects. The present study demonstrates the first molecular identification of a partial . Zophobas atratus UCP4-coding sequence and the functional characterisation of ZaUCP4 in the mitochondria of larval and pupal fat bodies of the beetle. ZaUCP4 shows a high similarity to predicted insect UCP4 isoforms and known mammalian UCP4s, both at the nucleotide and amino acid sequence levels. Bioenergetic studies clearly demonstrate UCP function in mitochondria from larval and pupal fat bodies. In non-phosphorylating mitochondria, ZaUCP activity was stimulated by palmitic acid and inhibited by the purine nucleotide GTP. In phosphorylating mitochondria, ZaUCP4 activity decreased the yield of oxidative phosphorylation. ZaUCP4 was immunodetected with antibodies raised against human UCP4 as a single 36-kDa band. A lower expression of ZaUCP4 at the level of mRNA and protein and a decreased ZaUCP4 activity were observed in the Z. atratus pupal fat body compared with the larval fat body. The different expression patterns and activity of ZaUCP4 during the larval-pupal transformation indicates an important physiological role for UCP4 in insect fat body development and function during insect metamorphosis. © 2012 Elsevier Inc.. Source

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