Insulin resistance associated with polycystic ovary syndrome - Is Pro12Ala polymorphism of the PPAR-γ gene involved? [Rezistenţa la insulinǎ asociatǎ sindromului ovarelor polichistice -este implicat polimorfismul Pro12Ala al genei PPAR-γ?]
Gliga F.,UMF Tirgu Mures |
Pascanu I.,UMF Tirgu Mures |
Stoian A.,UMF Tirgu Mures |
Nicola T.,County Emergency Clinical Hospital |
Banescu C.,UMF Tirgu Mures
Revista Romana de Medicina de Laborator | Year: 2012
Polycystic ovary syndrome (PCOS) is a complex and heterogeneous endocrine disorder with many unclear pathogenetic pathways. The aim of the present research was to investigate the potential implication of the Pro12Ala polymorphism of the PPAR-γ (peroxisome proliferator-activated receptor gamma) gene in the pathogenesis of PCOS, the possible relationship between Pro12Ala polymorphism and the hyperandrogenemia and insulin resistance. Material and methods. We carried out a case-control study involving 89 patients with 47 PCOS and 42 controls, with a mean age of 25.10±5.57 years. BMI (body mass index) was calculated. Blood samples were collected after a period of 12 hours fasting, in the early follicular phase of menstrual cycle, and serum glucose, insulin, testosterone and SHBG (sex hormone binding globulin) were measured. HOMA (homeostasis model assessment) and FAI (free androgen index) were also calculated. DNA was isolated from peripheral blood. PPAR-γ gene polymorphism was examined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The genotype was compared among the study groups using Fisher's test. A p-value of <0.05 was considered statistically significant. Results. The PPAR-γ genotypes and allele variants proved to be in equilibrium in both study groups. There was no difference shown in the distribution of the Pro12Ala polymorphism between PCOS and healthy controls. The insulin resistance degree and androgen indices had no significant modified values among the different genotypes of the Pro12Ala polymorphism. Conclusion. The outcome of our study does not support any association between the Pro12Ala polymorphism of the PPAR-γ gene and PCOS in Romanian women.