Robert L W.,Rutgers University |
Allen L.A.,Robert Wood Johnson Medical School UMDNJ
Journal of Cognitive Psychotherapy | Year: 2010
Somatization disorder is the most severe and refractory of the somatoform disorders. In this article, we provide an overview of somatization disorder, reviewing both the experimental psychopathology and treatment outcome literatures. We also describe a new psychosocial intervention that we developed to treat somatization disorder, affective-cognitive behavioral therapy. We attempt to place the treatment within the context of contemporary cognitive behavioral therapy. © 2010 Springer Publishing Company.
Nanda V.,Robert Wood Johnson Medical School UMDNJ |
Hsieh D.,Center for Advanced Biotechnology and Medicine |
Davis A.,Center for Advanced Biotechnology and Medicine
Methods in Molecular Biology | Year: 2013
Protein-protein interactions (PPI) play central roles in biological processes, motivating us to understand the structural basis underlying affinity and specificity. In this chapter, we focus on biochemical and computational design strategies of assessing and detecting PPIs of β-barrel outer membrane proteins (OMPs). A few case studies are presented highlighting biochemical techniques used to dissect the energetics of oligomerization and determine amino acids forming the key interactions of the PPI sites. Current computational strategies for detecting/predicting PPIs are introduced, and examples of computational and rational engineering strategies applied to OMPs are presented. © 2013 Springer Science+Business Media, LLC.
Deng J.,PLA Fourth Military Medical University |
Deng J.,Cardiovascular Research Institute |
Han Y.,Cardiovascular Research Institute |
Yan C.,Cardiovascular Research Institute |
And 4 more authors.
Apoptosis | Year: 2010
Bone marrow-derived mesenchymal stem cells (MSCs) have great potential for repair after myocardial infarction. However, poor viability of transplanted MSCs in the ischemic heart has limited their therapeutic potential. Cellular repressor of E1A-stimulated genes (CREG) has been identified as a potent inhibitor of apoptosis. The aim of this study was to investigate the anti-apoptotic effects of CREG on MSCs under hypoxic and serum deprivation (SD) conditions. We also investigated the potential mechanism(s) that may mediate the actions of CREG. All experiments were performed on rat bone marrow MSCs. Apoptosis was induced by exposure of cells to hypoxia/SD in a sealed GENbox hypoxic chamber. Effects of CREG were investigated in the absence or presence of inhibitors that target phosphoinositide 3-kinase (PI3K). We found that the overexpression of CREG markedly protected MSCs from hypoxia/SD-induced apoptosis through inhibition of the mitochondrial apoptotic pathway, leading to attenuation of caspase-3. Moreover, CREG enhanced Akt phosphorylation and decreased the expression of p53 in MSCs under hypoxic/SD conditions. The PI3K/Akt inhibitor LY294002 significantly increased the amount of p53 protein and attenuated the anti-apoptotic effects of CREG on MSCs. This study indicates that CREG is a novel and potent survival factor for MSCs, therefore, it may be a useful therapeutic adjunct for transplanting MSCs into damaged heart after myocardial infarction. © 2009 Springer Science+Business Media, LLC.
Gogineni R.R.,Robert Wood Johnson Medical School UMDNJ |
Gogineni R.R.,Cooper University Hospital |
Fallon A.E.,Fielding Graduate University |
Fallon A.E.,Drexel University |
And 2 more authors.
Child and Adolescent Psychiatric Clinics of North America | Year: 2010
This article reviews, consolidates, and enhances current knowledge about the issues and problems child and adolescent psychiatry international medical graduates face. Their training, work force issues, and establishment and advancement of professional identity are presented. Acculturation and immigration dynamics include facing prejudice and discrimination, social mirroring, and difficulties with language. Treatment issues are discussed with a special focus on therapeutic alliance, resistance, transference, countertransference, and child rearing practices. Recommendations for training and future goals are considered. © 2010 Elsevier Inc.
Glycosylation-independent binding to extracellular domains 11-13 of mannose-6-phosphate/insulin-like growth factor-2 receptor mediates the effects of soluble CREG on the phenotypic modulation of vascular smooth muscle cells
Li S.,Robert Wood Johnson Medical School UMDNJ
Journal of Molecular and Cellular Cardiology | Year: 2011
The bio-effects of cellular repressor of E1A-stimulated genes (CREG) have been proposed to depend on its N-glycosylation and binding to mannose-6-phosphate/insulin-like growth factor-2 receptor (M6P/IGF2R). The present study aimed to investigate the detailed mode and specific sites for their binding and the functional relevance of this binding in the phenotypic modulation of vascular smooth muscle cells (SMCs). Wild-type and glycosylation mutant human CREG (wtCREG and mCREG) proteins were expressed and isolated from HEK293 cells. CREG knocked-down SMCs were used to evaluate their biological activity. Both wtCREG and mCREG arrest cell cycle progression of CREG knocked-down SMCs when added to the culture medium. In vitro binding assay revealed that CREG bound to M6P/IGF2R extracellular domains 7-10 and 11-13 in a glycosylation-dependent and -independent manner, respectively. Further blocking experiments using soluble M6P/IGF2R fragments and M6P/IGF2R neutralizing antibody suggest that the binding to domains 11-13, as well as to 7-10, is adequate for CREG to modulate SMC proliferation. These data suggest that soluble CREG protein can exert its biological function via glycosylation-independent binding to the extracellular domains 11-13 of cell surface M6P/IGF2R, and thereby provide novel insights into CREG modulation of SMC phenotypic switching from contractile to proliferative. © 2010 Elsevier Ltd.