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Abel N.J.,St. George's University | Rogal G.J.,Saint Barnabas Medical Center | Saunders C.R.,UMDNJ NJ Medical School | Chamberlain R.S.,St. George's University | Chamberlain R.S.,Saint Barnabas Medical Center
Cardiology (Switzerland) | Year: 2013

Introduction: Octogenarians are often denied complex surgical intervention. We evaluated the rationality of this bias by comparing the outcomes of octogenarians undergoing aortic valve replacement (AVR) with or without coronary artery bypass grafting (CABG), to those of younger patients. Methods: Data on 476 patients (≥80 years) who underwent AVR or AVR/CABG were compared to the Society of Thoracic Surgeons (STS) database. Results: One hundred and seventeen octogenarians underwent AVR and 263 underwent AVR/CABG. Preoperative comorbidity rates were similar between these 2 respective groups, except for diabetes mellitus (18.8 vs. 30.4%, p = 0.02), previous cardiac stent placement (5.1 vs. 17.9%, p = 0.0006) and prior CABG (8.5 vs. 0.8%, p = 0.0002) and mortality did not differ significantly (5.1 vs. 7.6%, p = 0.51). Multivariate analysis identified preoperative chronic renal failure [odds ratio (OR) = 0.09, p < 0.048], postoperative arrhythmia (OR = 0.29, p < 0.022), sepsis (OR = 37.38, p < 0.000), pneumonia (OR = 8.29, p < 0.038) and renal failure (OR = 10.16, p < 0.000) with increased rates of inhospital mortality in AVR alone and AVR/CABG. Conclusion: AVR alone or AVR/CABG can be safely performed in patients ≥ 80 years with acceptable morbidity/mortality rates. An age of ≥ 80 years is not an independent risk factor predictive of increased inhospital mortality. Copyright © 2013 S. Karger AG, Basel.


Candelmo A.C.,Rutgers University | Deshpande A.,National Oceanic and Atmospheric Administration | Dockum B.,National Oceanic and Atmospheric Administration | Weis P.,UMDNJ N.J. Medical School | Weis J.S.,Rutgers University
Estuaries and Coasts | Year: 2010

Young-of-the-year (YOY) bluefish, Pomatomus saltatrix, reside in some contaminated estuaries of the mid-Atlantic bight during their early life history, and as a result of this exposure, they may bioaccumulate high levels of contaminants, including polychlorinated biphenyls (PCBs), pesticides, and methyl mercury. Young-of-the-year bluefish from the Tuckerton, NJ, area of Great Bay (TK) were fed daily in a laboratory with common prey fish, menhaden, and mummichog from two sites: TK (reference) or Hackensack River (HR) (contaminated). Bluefish fed HR prey and the HR prey themselves had significantly elevated concentrations of PCBs, pesticides, and total mercury compared to TK counterparts. The bluefish fed contaminated prey for 4 months displayed significantly reduced feeding, spontaneous activity, and growth compared to the bluefish fed TK prey. Alterations of bluefish behavior and growth from exposure to contaminants may have detrimental effects on migration, overwinter survival, and recruitment success. © 2010 Coastal and Estuarine Research Federation.


Das A.,UMDNJ NJ Medical School | Morales R.,UMDNJ NJ Medical School | Banday M.,UMDNJ NJ Medical School | Garcia S.,UMDNJ NJ Medical School | And 4 more authors.
RNA | Year: 2012

RNA-binding proteins that target mRNA coding regions are emerging as regulators of post-transcriptional processes in eukaryotes. Here we describe a newly identified RNA-binding protein, RBP42, which targets the coding region of mRNAs in the insect form of the African trypanosome, Trypanosoma brucei. RBP42 is an essential protein and associates with polysome-bound mRNAs in the cytoplasm. A global survey of RBP42-bound mRNAs was performed by applying HITS-CLIP technology, which captures protein-RNA interactions in vivo using UV light. Specific RBP42-mRNA interactions, as well as mRNA interactions with a known RNA-binding protein, were purified using specific antibodies. Target RNA sequences were identified and quantified using high-throughput RNA sequencing. Analysis revealed that RBP42 bound mainly within the coding region of mRNAs that encode proteins involved in cellular energy metabolism. Although the mechanism of RBP42's function is unclear at present, we speculate that RBP42 plays a critical role in modulating T. brucei energy metabolism. Published by Cold Spring Harbor Laboratory Press. Copyright © 2012 RNA Society.


Dulal K.,UMDNJ NJ Medical School | Silver B.,UMDNJ NJ Medical School | Zhu H.,UMDNJ NJ Medical School
Journal of Biomedicine and Biotechnology | Year: 2012

Bacterial artificial chromosome (BAC) technology has contributed immensely to manipulation of larger genomes in many organisms including large DNA viruses like human cytomegalovirus (HCMV). The HCMV BAC clone propagated and maintained inside E. coli allows for accurate recombinant virus generation. Using this system, we have generated a panel of HCMV deletion mutants and their rescue clones. In this paper, we describe the construction of HCMV BAC mutants using a homologous recombination system. A gene capture method, or gap repair cloning, to seize large fragments of DNA from the virus BAC in order to generate rescue viruses, is described in detail. Construction of rescue clones using gap repair cloning is highly efficient and provides a novel use of the homologous recombination-based method in E. coli for molecular cloning, known colloquially as recombineering, when rescuing large BAC deletions. This method of excising large fragments of DNA provides important prospects for in vitro homologous recombination for genetic cloning. Copyright © 2012 Kalpana Dulal et al.


Study design: A prospective, randomized, single-blind (for the examining physicians) comparative study of efficiency. The effectiveness of the topical thermal therapy was compared with the treatment with oral analgesics in the treatment of lower back pains has not been established so far. Objective: The comparison of effectiveness of the continuous packing therapy (40°C, 8 h/day) andeffectiveness of ibuprofen (1.200 mg/day) and acetaminophen (4.000 mg/day) in subjects with acute nonspecific lower back pains. Methods: The subjects (n=371) were randomly divided into groups treated with thermal packing (n=113), acetaminophen (n=113) and ibuprofen (n=106) with the objective to determine effectiveness and to compare these kids of therapy with oral placebo (n=20) or a cold packing of the back (n=19) considering the blinded character of the treatment. The target indices included the relief of pain, muscular tightness, mobility of lateral trunk and a degree of invalidity. The effectiveness was determined on the course of two days of therapy and two days of observation. Results: The pain relief with the thermal packing on day 1 of the therapy (mean = 2) was higher than in the group treated with ibuprofen (mean 1.51: P = 0.007) or acetaminophen (mean 1.32; P = 0.0001). A prolonged pain relief (day 3 and 4) for the thermal packing (mean 2.61) was also more pronounced than for ibuprofen (mean 1.68; P=0.0001) or acetaminophen (mean 1.95; P=0.0009). The flexibility of lateral trunk improved in the thermal packing (mean change 4.28cm) during the therapy (P=0.009 vs. acetaminophen [mean change 2.93cm], P=0.001) vs. ibuprofen [mean change 2.51cm]). The results were similar on day 4 of the study. On day one of the study the mitigation of the muscular tightness (mean 16.3) was higher than with acetaminophen (mean 10.5; P=0.001). Invalidity decreased on day 4 in the group treated with the thermal packing (mean 4.9) in comparison with ibuprofen (mean 2.7; P=0.01) and acetaminophen (mean 2.9; P=0.0007)/ None of these undesirable effect was significant. The highest degree (10.4) was reported in the group treated with ibuprofen. Conclusion: The uninterrupted low temperature packing therapy proved to be more effective than acetaminophen and ibuprofen in the therapy of lower back pains.


Kaposis sarcoma-associated herpesvirus (KSHV; also known as human herpesvirus 8 [HHV-8]) is the etiologic agent of Kaposis sarcoma (KS) and lymphoproliferative diseases. We previously demonstrated that the KSHV lytic switch protein Rta stimulates DNA binding of the cellular RBP-Jk/CSL protein, the nuclear component of the Notch pathway, on Rta target promoters. In the current study, we define the promoter requirements for formation of transcriptionally productive Rta/RBP-Jk/DNA complexes. We show that highly pure Rta footprints 7 copies of a previously undescribed repetitive element in the promoter of the essential KSHV Mta gene. We have termed this element the CANT repeat. CANT repeats are found on both strands of DNA and have a consensus sequence of ANTGTAACANT(A/T)(A/T)T. We demonstrate that Rta tetramers make high-affinity interactions (i.e., nM) with 64 bp of the Mta promoter but not single CANT units. The number of CANT repeats, their presence in palindromes, and their positions relative to the RBP-Jk binding site determine the optimal target for Rta stimulation of RBP-Jk DNA binding and formation of ternary Rta/RBP-Jk/DNA complexes. DNA binding and tetramerization mutants of Rta fail to stimulate RBP-Jk DNA binding. Our chromatin immunoprecipitation assays show that RBP-Jk DNA binding is broadly, but selectively, stimulated across the entire KSHV genome during reactivation. We propose a model in which tetramerization of Rta allows it to straddle RBP-Jk and contact repeat units on both sides of RBP-Jk. Our study integrates high-affinity Rta DNA binding with the requirement for a cellular transcription factor in Rta transactivation.


PubMed | UMDNJ NJ Medical School
Type: Journal Article | Journal: Journal of back and musculoskeletal rehabilitation | Year: 2012

To determine the relationship of previous lower extremity injury and the measured ratio of hip abduction to extension strength in collegiate athletes.Cohort study of college athletes at time of pre-participation screening physical.An NCAA Division I college.Two hundred and thirty-six college athletes from a NCAA Division I school (162) males and (74) females.The ratio of maximal hip abduction to extension strength was calculated, following raw data collection with a specially designed dynamometer anchoring station. Injury to the lower extremities, in the past year, was recorded via personal interview at the time of screening and verified by review of previous injury records.A significant difference in the ratio of hip abduction to extension strength was noted on the left lower extremity of athletes with reported lower extremity (LE) injury compared to those without injury. Upon further review of data, hip extension weakness appears to be the likely cause of this difference.Athletes with reported lower extremity injury demonstrated a significant residual difference in the ratio of hip abduction to extension strength. This may be the result of injury related muscle weakness, altered muscle firing patterns, central inhibition or unknown compensatory strategies which all may be risk factors for recurrent injury. Further research is underway to identify the cause/effect relationship of this finding.This study may advance our understanding of potential compensatory strategies about the hip which theoretically may result from previous lower extremity injury or injuries which are incompletely rehabilitated. Additionally, this study provides some reasoning to support the screening of hip strength during the pre-participation physical, as it may be an important factor to prevent recurrent injury.


PubMed | UMDNJ NJ Medical School
Type: | Journal: Journal of biomedicine & biotechnology | Year: 2012

Bacterial artificial chromosome (BAC) technology has contributed immensely to manipulation of larger genomes in many organisms including large DNA viruses like human cytomegalovirus (HCMV). The HCMV BAC clone propagated and maintained inside E. coli allows for accurate recombinant virus generation. Using this system, we have generated a panel of HCMV deletion mutants and their rescue clones. In this paper, we describe the construction of HCMV BAC mutants using a homologous recombination system. A gene capture method, or gap repair cloning, to seize large fragments of DNA from the virus BAC in order to generate rescue viruses, is described in detail. Construction of rescue clones using gap repair cloning is highly efficient and provides a novel use of the homologous recombination-based method in E. coli for molecular cloning, known colloquially as recombineering, when rescuing large BAC deletions. This method of excising large fragments of DNA provides important prospects for in vitro homologous recombination for genetic cloning.


PubMed | UMDNJ NJ Medical School
Type: Journal Article | Journal: RNA (New York, N.Y.) | Year: 2012

RNA-binding proteins that target mRNA coding regions are emerging as regulators of post-transcriptional processes in eukaryotes. Here we describe a newly identified RNA-binding protein, RBP42, which targets the coding region of mRNAs in the insect form of the African trypanosome, Trypanosoma brucei. RBP42 is an essential protein and associates with polysome-bound mRNAs in the cytoplasm. A global survey of RBP42-bound mRNAs was performed by applying HITS-CLIP technology, which captures protein-RNA interactions in vivo using UV light. Specific RBP42-mRNA interactions, as well as mRNA interactions with a known RNA-binding protein, were purified using specific antibodies. Target RNA sequences were identified and quantified using high-throughput RNA sequencing. Analysis revealed that RBP42 bound mainly within the coding region of mRNAs that encode proteins involved in cellular energy metabolism. Although the mechanism of RBP42s function is unclear at present, we speculate that RBP42 plays a critical role in modulating T. brucei energy metabolism.

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