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Maji A.K.,Vidyasagar University | Samanta S.K.,Jadavpur University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Oriental Pharmacy and Experimental Medicine | Year: 2014

Stereospermum suaveolens (Roxb.) DC. is used in various Ayurvedic formulations to treat variety of disorders including inflammations, asthma, blood disorders, fevers, liver disorders etc. Quantification of the bioactive compound in S. suaveolens root extract (SSRE) was determined through RP-HPLC, in order to standardize the plant material with optimal concentration of known active constituents present there in. The immunomodulatory potential of SSRE was determined for its effects on non-specific immune functions against sheep red blood cells antigenic challenge using in-vivo models. The assay included total and differential leukocyte counts, nitroblue-tetrazolium reduction test, neutrophil adhesion test, phagocytic activity and delayed type hypersensitivity (DTH) reaction. In RP-HPLC analysis, the contents of dehydro-α-lapachone and lapachol in SSRE was found to be 0.043 ± 0.003 and 0.16 ± 0.002 % (w/w), respectively. Standardized SSRE (100-300 mg/kg) increased the total leukocyte count and the population of monocyte and neutrophil in rats. Further, treatment with SSRE increased the neutrophil adhesion to nylon fibres, DTH response, phagocytic activity and intracellular killing potential of phagocytes in a dose dependent manner. The immunostimulatory potential of SSRE at 300 mg/kg was found to be very significant (p < 0.001) in compared to the control. These findings suggest that SSRE can able to stimulate the innate defence mechanisms of an individual and it can be considered as an alternative therapy to boost the innate immune functions during the impaired immunological conditions. © 2013 Institute of Korean Medicine, Kyung Hee University.


Gantait A.,Jadavpur University | Maji A.,Ulysses Research Foundation | Barman T.,Jadavpur University | Banerji P.,Ulysses Research Foundation | And 2 more authors.
Natural Product Research | Year: 2012

Capsicum annuum L. (family: Solanaceae) possesses therapeutic benefits for the treatment of rheumatism, neuropathy, psoriasis, flatulence and so on. In this study fruits of four different varieties of C. annuum from four different geographical regions in India were evaluated based on their total content of capsaicin. Ethanol extracts of the fruits were used. HPTLC plates were developed in a mobile phase containing benzene, ethyl acetate and methanol (75:20:5). Densitometric scanning was performed at a wavelength of 283nm in the absorbance mode. The calibration curve was described by the equation Y=393.587+3.836X with a correlation coefficient (r) of 0.99890. The content of capsaicin in Nagaland, Manipur, West Bengal and Shimla varieties was found to be 3.71%, 1.78%, 0.54% and 0.06%, respectively. The developed densitometric method was found to be specific, accurate and precise. A recovery study and precision showed low levels of %RSD values. The linearity range of the curve for capsaicin was found to be 300-900ng per spot. The limit of detection and the limit of quantification values were determined to be 31 and 94ng, respectively, proving the sensitivity of the method. Thus the method can be used to control the total content of capsaicin on an industrial scale. © 2012 Copyright Taylor and Francis Group, LLC.


Maji A.K.,Vidyasagar University | Maity N.,Jadavpur University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
International Journal of Phytomedicine | Year: 2013

Asteracantha longifolia (L.) Nees. (Acanthaceae) is a well known medicinal plant of Indian traditional medicines. The aim of this work was to develop a validated reversed phase-high performance liquid chromatography (RP-HPLC) method for the quantitative determination of betulin in A. longifolia extract. The analysis was performed by RP-HPLC on Luna C18 (2) 100 Å, 250 x 4.6 mm column under isocratic elution of acetonitrile and water (80:20, v/v) with a flow rate of 1.0 ml/min and the total run time was 20 min. The column temperature was adjusted at 25 C and the detection wavelength was set at 210 nm. The method was validated for suitability, specificity, accuracy, precision, limits of detection and quantification (LOD and LOQ), robustness and ruggedness. The betulin content in A. longifolia extract was found to be 15.96 + 0.34% (w/w). The calibration curve was linear over a concentration range of 10-125 μg/ml (r2 = 0.997) and the recovery range was 98.29-99.59%. The LOD and LOQ were 1.11 and 7.35 μg/ml, respectively. The intra- and inter-day assay precisions were satisfactory and the relative standard deviations were found to be always less than 2%. The developed method was found to be simple, sensitive, accurate, robust and rugged for the quantification of betulin. This validated method can be useful for the routine quality control analysis of betulin content in A. longifolia extract and its formulations.


Maji A.K.,Vidyasagar University | Pandit S.,Ulysses Research Foundation | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Natural Product Research | Year: 2014

Pueraria tuberosa (Willd.) DC is a perennial herb commonly known as vidarikanda, distributed throughout south east Asia. The plant's tuber is widely used in ethanomedicine as well as in traditional systems of medicine, particularly in ayurveda. It has been used in various ayurvedic formulations as restorative tonic, antiaging, spermatogenic and immune booster and has been recommended for the treatment of cardiovascular diseases, hepatosplenomegaly, fertility disorders, menopausal syndrome, sexual debility and spermatorrhoea. Numerous bioactive phytochemicals, mostly isoflavonoids such as puerarin, genistein, daidzein, tuberosin and so on have been identified in the tuber. In vivo and in vitro studies have provided the support against traditional demands of the tuber as spermatogenic, immune booster, aphrodisiac, anti-inflammatory, cardiotonic and brain tonic. However, further studies are required to define the active phytochemical compositions and to validate its clinical utilisation in the herbal formulations for human uses. This review provides an overview of traditional applications, current knowledge on the phytochemistry, pharmacology and toxicology of P. tuberosa. This review also provides plausible hypotheses about how various isoflavones particularly puerarin, genistein and daidzein, individually or collectively, may be responsible for the therapeutic potential against a wide range of ailments. © 2014 © 2014 Taylor & Francis.


Banerjee D.,Vidyasagar University | Maji A.K.,Vidyasagar University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation
Research Journal of Phytochemistry | Year: 2012

Barleria prionitis Linn. (Family: Acanthaceae) is a well-known perennial, Ayurvedic herb distributed in the tropical Asia, Africa and Yemen. The whole plant or its specific parts (leaf, stem, root, bark and flower) has been utilized for treatment of toothache, catarrhal affections, whooping cough, inflammations, glandular swellings, urinary infection, jaundice, fever, gastrointestinal disorders and as diuretic and tonic. A wide range of phytochemical constituents including balarenone, pipataline prionisides, barlerinoside, verbascoside, shanzhiside methyl ester, barlerin, acetylbarlerin, lupulinoside, scutellarein have been isolated from the different parts of this plant. Extracts and isolated phytochemicals from this plant have been found to posses wide range of pharmacological include antimicrobial, anthelmintic, antifertility, antioxidant, antidiabetic, anti-inflammatory, anti-arthritic, cytoprotective, hep atoprotective, diuretic, antidiarrhoeal, enzyme inhibitory and anti-nociceptive activities without any toxic effects. So, in this review attempt has being made to highlight the traditional uses, phytochemistry, pharmacology and toxicity of this plant. © 2012 Academic Journals Inc.


Maji A.K.,Vidyasagar University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Oriental Pharmacy and Experimental Medicine | Year: 2015

Wedelia chinensis is a medicinal herb, traditionally used to treat variety of immunological disorders. The herb and its coumestans, wedelolactone (WL) and demethylwedelolactone (DWL) were demonstrated to possess several biological actions. This work was aimed to evaluate the in vitro immunomodulatory effect of DWL as well as the standardized Wedelia chinensis extract (WCE). In vitro immunomodulatory potential was evaluated by assessing the effects on Compound 48/80 (C 48/80)-induced de-granulation of mast cell and LPS-stimulated production of NO, pro-inflammatory cytokines and the expression of costimulatory molecules in macrophages. RP-HPLC analysis of WCE indicated the abundance of WL (1.96 ± 0.03 %, w/w) and DWL (0.61 ± 0.005 %, w/w). Results of the present investigation showed that WCE and DWL dose dependently inhibited the de-granulation of mast cells induced by C 48/80. Additionally, WCE and DWL were also inhibited the production of NO, pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6 and the expression of costimulatory molecules such as CD40, CD80 and CD86 in LPS-stimulated macrophages. These findings suggest that WCE has modulatory effect on various immune-inflammatory mediators mainly due to the presence of WL along with the lesser amount of DWL. Therefore, this plant as well as its coumestans, WL and DWL can be exploited as alternative new therapeutics for various inflammatory diseases. © 2015, Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media Dordrecht.


Maji A.K.,Vidyasagar University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Journal of Herbs, Spices and Medicinal Plants | Year: 2015

Effects of standardized Wrightia tinctoria seeds extract (WTSE) and Stereospermum suaveolens root extract (SSRE) on erythrocyte membrane stabilization, mast cell protection, nitric oxide, and pro-inflammatory cytokines production were evaluated by in vitro methods. WTSE and SSRE were standardized against chlorogenic acid and lapachol, respectively, using reverse-phase high-performance liquid chromatography. Standardized WTSE and SSRE inhibited erythrocyte membrane lysis, mast cell degranulation, and LPS-stimulated nitric oxide and pro-inflammatory cytokine (IL-β, IL-6 and TNF-α) production by RAW 264.7 macrophage cells in a dose-dependent manner. © Taylor & Francis Group, LLC.


Maji A.K.,Ulysses Research Foundation | Banerji P.,Ulysses Research Foundation
Journal of Complementary and Integrative Medicine | Year: 2016

Dietary spices and their active constituents provide various beneficial effects on the gastrointestinal system by variety of mechanisms such as influence of gastric emptying, stimulation of gastrointestinal defense and absorption, stimulation of salivary, intestinal, hepatic, and pancreatic secretions. Capsicum annuum (Solanaceae), commonly known as chilli, is a medicinal spice used in various Indian traditional systems of medicine and it has been acknowledged to treat various health ailments. Therapeutic potential of chilli and capsaicin were well documented; however, they act as double-edged sword in many physiological circumstances. In traditional medicine chilli has been used against various gastrointestinal complains such as dyspepsia, loss of appetite, gastroesophageal reflux disease, gastric ulcer, and so on. In chilli, more than 200 constituents have been identified and some of its active constituents play numerous beneficial roles in various gastrointestinal disorders such as stimulation of digestion and gastromucosal defense, reduction of gastroesophageal reflux disease (GERD) symptoms, inhibition of gastrointestinal pathogens, ulceration and cancers, regulation of gastrointestinal secretions and absorptions. However, further studies are warranted to determine the dose ceiling limit of chilli and its active constituents for their utilization as gastroprotective agents. This review summarizes the phytochemistry and various gastrointestinal benefits of chilli and its various active constituents. © 2016 by De Gruyter.

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