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Maji A.K.,Vidyasagar University | Samanta S.K.,Jadavpur University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Oriental Pharmacy and Experimental Medicine | Year: 2014

Stereospermum suaveolens (Roxb.) DC. is used in various Ayurvedic formulations to treat variety of disorders including inflammations, asthma, blood disorders, fevers, liver disorders etc. Quantification of the bioactive compound in S. suaveolens root extract (SSRE) was determined through RP-HPLC, in order to standardize the plant material with optimal concentration of known active constituents present there in. The immunomodulatory potential of SSRE was determined for its effects on non-specific immune functions against sheep red blood cells antigenic challenge using in-vivo models. The assay included total and differential leukocyte counts, nitroblue-tetrazolium reduction test, neutrophil adhesion test, phagocytic activity and delayed type hypersensitivity (DTH) reaction. In RP-HPLC analysis, the contents of dehydro-α-lapachone and lapachol in SSRE was found to be 0.043 ± 0.003 and 0.16 ± 0.002 % (w/w), respectively. Standardized SSRE (100-300 mg/kg) increased the total leukocyte count and the population of monocyte and neutrophil in rats. Further, treatment with SSRE increased the neutrophil adhesion to nylon fibres, DTH response, phagocytic activity and intracellular killing potential of phagocytes in a dose dependent manner. The immunostimulatory potential of SSRE at 300 mg/kg was found to be very significant (p < 0.001) in compared to the control. These findings suggest that SSRE can able to stimulate the innate defence mechanisms of an individual and it can be considered as an alternative therapy to boost the innate immune functions during the impaired immunological conditions. © 2013 Institute of Korean Medicine, Kyung Hee University.

Banerjee D.,Vidyasagar University | Maji A.K.,Vidyasagar University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation
Research Journal of Phytochemistry | Year: 2012

Barleria prionitis Linn. (Family: Acanthaceae) is a well-known perennial, Ayurvedic herb distributed in the tropical Asia, Africa and Yemen. The whole plant or its specific parts (leaf, stem, root, bark and flower) has been utilized for treatment of toothache, catarrhal affections, whooping cough, inflammations, glandular swellings, urinary infection, jaundice, fever, gastrointestinal disorders and as diuretic and tonic. A wide range of phytochemical constituents including balarenone, pipataline prionisides, barlerinoside, verbascoside, shanzhiside methyl ester, barlerin, acetylbarlerin, lupulinoside, scutellarein have been isolated from the different parts of this plant. Extracts and isolated phytochemicals from this plant have been found to posses wide range of pharmacological include antimicrobial, anthelmintic, antifertility, antioxidant, antidiabetic, anti-inflammatory, anti-arthritic, cytoprotective, hep atoprotective, diuretic, antidiarrhoeal, enzyme inhibitory and anti-nociceptive activities without any toxic effects. So, in this review attempt has being made to highlight the traditional uses, phytochemistry, pharmacology and toxicity of this plant. © 2012 Academic Journals Inc.

Maji A.K.,Vidyasagar University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Oriental Pharmacy and Experimental Medicine | Year: 2015

Wedelia chinensis is a medicinal herb, traditionally used to treat variety of immunological disorders. The herb and its coumestans, wedelolactone (WL) and demethylwedelolactone (DWL) were demonstrated to possess several biological actions. This work was aimed to evaluate the in vitro immunomodulatory effect of DWL as well as the standardized Wedelia chinensis extract (WCE). In vitro immunomodulatory potential was evaluated by assessing the effects on Compound 48/80 (C 48/80)-induced de-granulation of mast cell and LPS-stimulated production of NO, pro-inflammatory cytokines and the expression of costimulatory molecules in macrophages. RP-HPLC analysis of WCE indicated the abundance of WL (1.96 ± 0.03 %, w/w) and DWL (0.61 ± 0.005 %, w/w). Results of the present investigation showed that WCE and DWL dose dependently inhibited the de-granulation of mast cells induced by C 48/80. Additionally, WCE and DWL were also inhibited the production of NO, pro-inflammatory cytokines such as TNF-α, IL-1β and IL-6 and the expression of costimulatory molecules such as CD40, CD80 and CD86 in LPS-stimulated macrophages. These findings suggest that WCE has modulatory effect on various immune-inflammatory mediators mainly due to the presence of WL along with the lesser amount of DWL. Therefore, this plant as well as its coumestans, WL and DWL can be exploited as alternative new therapeutics for various inflammatory diseases. © 2015, Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media Dordrecht.

Maji A.K.,Vidyasagar University | Mahapatra S.,Vidyasagar University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
Journal of Herbs, Spices and Medicinal Plants | Year: 2015

Effects of standardized Wrightia tinctoria seeds extract (WTSE) and Stereospermum suaveolens root extract (SSRE) on erythrocyte membrane stabilization, mast cell protection, nitric oxide, and pro-inflammatory cytokines production were evaluated by in vitro methods. WTSE and SSRE were standardized against chlorogenic acid and lapachol, respectively, using reverse-phase high-performance liquid chromatography. Standardized WTSE and SSRE inhibited erythrocyte membrane lysis, mast cell degranulation, and LPS-stimulated nitric oxide and pro-inflammatory cytokine (IL-β, IL-6 and TNF-α) production by RAW 264.7 macrophage cells in a dose-dependent manner. © Taylor & Francis Group, LLC.

Maji A.K.,Vidyasagar University | Maity N.,Jadavpur University | Banerji P.,Ulysses Research Foundation | Banerjee D.,Vidyasagar University
International Journal of Phytomedicine | Year: 2013

Asteracantha longifolia (L.) Nees. (Acanthaceae) is a well known medicinal plant of Indian traditional medicines. The aim of this work was to develop a validated reversed phase-high performance liquid chromatography (RP-HPLC) method for the quantitative determination of betulin in A. longifolia extract. The analysis was performed by RP-HPLC on Luna C18 (2) 100 Å, 250 x 4.6 mm column under isocratic elution of acetonitrile and water (80:20, v/v) with a flow rate of 1.0 ml/min and the total run time was 20 min. The column temperature was adjusted at 25 C and the detection wavelength was set at 210 nm. The method was validated for suitability, specificity, accuracy, precision, limits of detection and quantification (LOD and LOQ), robustness and ruggedness. The betulin content in A. longifolia extract was found to be 15.96 + 0.34% (w/w). The calibration curve was linear over a concentration range of 10-125 μg/ml (r2 = 0.997) and the recovery range was 98.29-99.59%. The LOD and LOQ were 1.11 and 7.35 μg/ml, respectively. The intra- and inter-day assay precisions were satisfactory and the relative standard deviations were found to be always less than 2%. The developed method was found to be simple, sensitive, accurate, robust and rugged for the quantification of betulin. This validated method can be useful for the routine quality control analysis of betulin content in A. longifolia extract and its formulations.

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