Ultizyme International Ltd.

Meguro-ku, Japan

Ultizyme International Ltd.

Meguro-ku, Japan
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Patent
ARKRAY Inc., Ultizyme International Ltd. and Tanaka Kikinzoku Kogyo K.K. | Date: 2017-02-15

A measuring method of measuring a concentration of a target ingredient in a sample by using a sensor including an interdigitated array electrode that includes a first electrode having a first comb tooth and a second electrode having a second comb tooth, and a reagent layer on the interdigitated array electrode, in which the first comb tooth and the second comb tooth are alternately arrayed, themeasuringmethodincluding: astepofapplyingavoltage to between the first electrode and the second electrode; a step of measuring a first current value of an electric current flowing between the first electrode and the second electrode; a step of measuring a second current value of the current flowing between the first electrode and the second electrode; a step of calculating the concentration of the target ingredient in the sample, based on a third current value; a step of calculating a correction value, based on the first current value and the second current value; and a step of correcting the concentration of the target ingredient in the sample, based on the correction value.


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2016-10-14

A biosensor according to an embodiment includes: a working electrode; a counter electrode; a reference electrode; and a detection layer contacting the working electrode and containing a crosslinking agent, an electrically conductive polymer, and an enzyme transferring and receiving electrons to and from the working electrode, the reference electrode being a polarized electrode.


Patent
ARKRAY Inc., Ultizyme International Ltd. and Tanaka Kikinzoku Kogyo K.K. | Date: 2016-08-09

A measuring method of measuring a concentration of a target ingredient in a sample by using a sensor including an interdigitated array electrode that includes a first electrode and a second electrode, and a reagent layer on the interdigitated array electrode, the measuring method includes: applying a voltage to between the first electrode and the second electrode; measuring a first current value of an electric current flowing between the first electrode and the second electrode; measuring a second current value of the current flowing between the first electrode and the second electrode; calculating the concentration of the target ingredient in the sample, based on a third current value; calculating a correction value, based on the first current value and the second current value; and a step of correcting the concentration of the target ingredient in the sample, based on the correction value.


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2017-05-03

The present invention provides a mutant-type glucose dehydrogenase having glucose dehydrogenase activity and having decreased reactivity with xylose, wherein said mutant-type glucose dehydrogenase comprises a mutant-type -subunit comprising an amino acid sequence of 520 to 550 amino acids containing the following peptide sequences (i) to (v) in this order, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in the peptide sequence (iii), the histidine at position 4 in the peptide sequence (iv), and the asparagine at position 4 in the peptide sequence (v) is/are substituted with another/other amino acid(s):(i) Val/Ile Val/Ile Val/Ile Gly Ser Gly Val Ala Gly (SEQ ID NO:21);(ii) Cys Cys Gly Asn Asn Asn Cys Met Pro Ile Cys (SEQ ID NO:22);(iii) Val Gly Arg Asn Leu Met Asp His Pro Gly Thr Gly (SEQ ID NO:23);(iv) Lys Lys Ile/Leu His Leu Ser Asn (SEQ ID NO:24);(v) Phe Ala Pro/Asn Asn Asn His Ile (SEQ ID NO:25).


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2016-10-14

An enzyme electrode includes an electrode, and a detection layer which contacts the electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the electrode and does not contain an electron transfer subunit.


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2016-10-28

The present invention provides a mutant-type glucose dehydrogenase having glucose dehydrogenase activity and having decreased reactivity with xylose, wherein said mutant-type glucose dehydrogenase comprises a mutant-type -subunit comprising an amino acid sequence of 520 to 550 amino acids comprising an amino acid sequence of 520 to 550 amino acids comprising SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24 and SEQ ID NO: 25 in this order from N-terminus to C-terminus, except that one or more amino acid residue(s) selected from the group consisting of the glycine at position 10 in SEQ ID NO: 23, the histidine at position 4 in SEQ ID NO: 24, and the asparagine at position 4 in SEQ ID NO: 25 is/are substituted with another/other amino acid(s).


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2017-04-19

A biosensor includes: a working electrode; a counter electrode; a reference electrode; and a detection layer contacting the working electrode and containing a crosslinking agent, an electrically conductive polymer, and an enzyme transferring and receiving electrons to and from the working electrode, the reference electrode being a polarized electrode.


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2017-04-19

A biosensor includes a plurality of electrodes including a working electrode, and a detection layer containing an enzyme for exchanging electrons with the working electrode, a crosslinking agent and an electrically conductive polymer and having a contact area with the working electrode defined by a predetermined area.


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2017-04-19

A biosensor includes a plurality of electrodes including a working electrode, and a detection layer which is immobilized on the working electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the working electrode. The working electrode is in an open system.


Patent
ARKRAY Inc. and Ultizyme International Ltd. | Date: 2017-04-19

An enzyme electrode including an electrode, and a detection layer which contacts the electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the electrode and does not contain an electron transfer subunit.

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