Saratov, Russia
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Nechaeva O.V.,Saratov State Medical University | Shurshalova N.F.,Chernyshevsky Saratov State University | Tikhomirova E.I.,Saratov State Technical University | Plotnikov O.P.,Ul Universitetskaya 46
Eksperimental'naya i Klinicheskaya Farmakologiya | Year: 2016

Complex assessment of the toxicity of new heterocyclic compounds (belonging to phenylpentenediones, chalcones, polyfunctional substituted esters, enamines, and semicarbazones) possessing high antimicrobial activity has been carried out on biotest objects and white laboratory mice. Preparations with low indices of acute toxicity were found in the groups of phenylpentenediones (2,4-dichloro-1,3,5-triphenyl-2-pentene-1,5-dione), chalcones (3(4-(dimethylamino) benzylidene) pentane-2,4-dione), polyfunctional substituted esters (2,2′-di-(3-(3-methoxy-4-hydroxyphenyl)-2,4-diacetyl-5-hydroxy-5-methylcyclogex-l-yl)-ethyl oxide) and enamines (ethyl-2-methyl-4-(1-piperidyl)-6-(3-nitrophenyl) cyclohexa-1,3-dienecarboxylate-dienamine), which can be considered as potential effective chemotherapeutic means for treating causative agents of infectious diseases.


Matrosov A.N.,Ul Universitetskaya 46 | Kuznetsov A.A.,Ul Universitetskaya 46 | Knyazeva T.V.,Ul Universitetskaya 46
Russian Journal of Ecology | Year: 2011

A concept of population control is proposed as applied to rodent carriers of plague and other infections with natural focality. The concept is based on the generalization of long-term experience gained in natural plague foci, differentiation of the enzootic territory with respect to the level of epidemic risk, analysis of the current state of rodent populations, and the necessity of biodiversity conservation in natural biocenoses. © 2011 Pleiades Publishing, Ltd.


Oparin M.L.,RAS Severtsov Institute of Ecology | Oparina O.S.,RAS Severtsov Institute of Ecology | Matrosov A.N.,Ul Universitetskaya 46 | Kuznetsov A.A.,Ul Universitetskaya 46
Biology Bulletin | Year: 2011

The steppe theriofauna of the Volga-Ural interfluve has been changing during the past century under the effect of both natural and anthropogenic factors. These changes have manifested themselves most clearly in the desert Caspian steppes and the dry steppes of the Transvolga Syrt Plain. A total of 66 mammal species are listed for this region. Some of them have disappeared while others have appeared anew, with their abundance ratio fluctuating significantly. Over the past 50 years, 15 new species have been recorded in the Transvolga fauna. One-third of them have come from the south and represent the desert faunal assemblage, while two-thirds are representatives of the forest assemblage that have come from the north. © 2011 Pleiades Publishing, Ltd.


Boolgakova E.G.,Ul Universitetskaya 46 | Krasnov Y.M.,Ul Universitetskaya 46 | Sukhonosov I.Y.,Ul Universitetskaya 46 | Gaeva A.V.,Ul Universitetskaya 46 | And 4 more authors.
Molecular Genetics, Microbiology and Virology | Year: 2016

The porin gene, which is adjacent to the pigmentation region (pgm), is usually damaged by IS100 element in highly virulent Yersinia pestis strains. In addition, the pgm region, which carries the genes responsible for virulence (high pathogenicity island) and biofilm generation (hms-operon), is flanked by direct IS100 copies (causing its destabilization). The study of distribution of intact and truncated porin genes was conducted among 240 Y. pestis strains from 39 natural foci of Russia and countries of the near abroad and 68 Yersinia pseudotuberculosis strains from different geographical regions. Most highly virulent Y. pestis strains and some phylogenetic Y. pseudotuberculosis lines of O:1 serotype contain truncated porin genes. At the same time, deletion of the pgm region by flanked IS100 in Y. pseudotuberculosis is impossible, since IS100 is integrated in the porin gene in an orientation opposite to that of Y. pestis. The intact porin gene is carried by all Y. pestis strains with low epidemic significance and certain phylogenetic lines of highly virulent Y. pestis strains from desert foci and Caspian sandy focus, as well as most Y. pseudotuberculosis strains of O:1 serotype. A continuous deletion, which includes the porin gene and a part of the astE gene, was detected in less virulent Y. pseudotuberculosis strains of O:3 serotype. The nucleotide sequence of porin genes is identical in Y. pestis and Y. pseudotuberculosis strains from different geographical regions. Three porin gene allele only differ by IS100 integration site and orientation or absence of its integration. The nucleotide sequence of IS100 introduced in the porin gene of Yersinia has small differences only for two Y. pestis strains isolated in America. The correlation of low frequency of Hms-mutants with the intact porin gene state in Y. pestis and the absence of such a correlation in Y. pseudotuberculosis were established. © 2016, Allerton Press, Inc.


Smirnova N.I.,Ul Universitetskaya 46 | Kul'shan' T.A.,Ul Universitetskaya 46 | Krasnov Y.M.,Ul Universitetskaya 46
Molecular Genetics, Microbiology and Virology | Year: 2015

MLVA typing was performed for five variable loci of the 52 Vibrio cholerae El Tor biovar strains isolated before the onset and in different periods of the seventh cholera pandemic, as well as for eight strains of the classical V. cholerae biovar—the causative agent of the preceding Asiatic cholera pandemics. It has been shown that the studied strains, which differ in their molecular genetic properties and are characterized by 38 MLVA types, form seven clonal clusters. Clusters I and II were formed, respectively, by V. cholerae strains of the classical biovar and prepandemic V. cholerae strains, whereas clusters III–VII were formed by V. cholerae El Tor biovar strains isolated in different periods of the latest pandemic. It has been shown that the MLVA typing allows us to clearly differentiate V. cholerae El Tor biovar strains into typical and genetically altered strains. The genetically altered strains are characterized by higher virulence compared with the typical strains, as well as the presence of the ctxB gene of the classical cholera vibrio in the genome. The study showed the possibility of differentiating genovariants isolated in different periods and having different structures of pandemicity island VSP-II. Since the VSP-II structure directly correlates with the epidemic potential of the strains, the possibility of differentiating isolates with intact and deleted VSP-II using the MLVA method deserves further research. Based on the obtained results, we can propose a polyclonal origin of different strains of genovariants. © 2015, Allerton Press, Inc.

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