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Rozova E.V.,Bogomolets Institute of Physiolgoy of the NAS of Ukraine | Pokhylko V.I.,Ukrainian Medical Stomatological Academy | Dubovaya M.G.,Bogomolets Institute of Physiolgoy of the NAS of Ukraine
Neurophysiology | Year: 2016

We examined changes in the morphofunctional state of the mitochondria (MCh) and immunohistochemical peculiarities of brainstem neurons in rat offspring exposed to experimental prenatal (intrauterine) hypoxia of different severity, moderate and strong. This was provided by exposure of pregnant females to O2/N2 respiratory mixtures containing 12 and 7% O2, respectively. Experimental groups included 20 one-month-old rats (offspring of 9 females, control and subjected to hypoxia). We estimated the ultrastructural characteristics of the MCh and also expression of the CD95 APO-1/Fas and Bcl-2 genes modulating the intensity of apoptosis and mitoptosis in these cells. Severe intrauterine hypoxia resulted in the development of structural distress in the MCh of brainstem cells; all stages of MCh degradation, from swelling to complete dissipation, were observed. Juvenile forms of these organelles were absent. Mosaic-like destruction of myelin with manifestations of edema was observed. After the moderate prenatal hypoxia, about half of the changes in the MCh ultrastructure could be qualified as directed toward an increase in the compensatory capabilities of the MCh apparatus. In rats after moderate hypoxic influence, levels of expression of the CD95 APO-1/Fas and Bcl-2 genes were indicative of a greater readiness of the neurons to apoptosis and decrease in the probability to inhibition of the respective MCh pathway in brainstem neurocites. At the same time, the MCh and neurocites of animals subjected to severe intrauterine hypoxia demonstrated decreased trends toward mitoptosis and apoptosis, respectively. The obtained results characterizing the effects of intrauterine hypoxia of different levels on the formation of structural/functional changes in the MCh of brainstem cells can be taken into account in the process of development of novel approaches to the treatment of MCh diseases. © 2016 Springer Science+Business Media New York

PubMed | Ukrainian Medical Stomatological Academy, Company PHARMASOFT and Ukrainian Academy of Sciences
Type: Journal Article | Journal: Biomeditsinskaia khimiia | Year: 2015

Magnetite nanoparticles (NPs) are studied as agents for magnetic resonance imaging, hyperthermia of malignant tumors, targeted drug delivery as well as anti-anemic action. One of the main problems of such NPs is their aggregation that requires creation of methods for magnetite NPs stabilization during preparation of liquid medicinal forms on their basis. The present work is devoted to the possibility of mexidol (2-ethyl-6-methyl-3-hydroxypyridine succinate) use for solubilization of magnetite NPs in hydrophilic medium. For this purpose, the condensate produced by electron-beam evaporation and condensation, with magnetite particles of size 5-8 nm deposited into the crystals of sodium chloride were used in conjunction with substance of mexidol (2-ethyl-6-methyl-3-hydroxypyridine succinate), and low molecular weight polyvinylpyrrolidone (PVP). The NP condensate was dispersed in distilled water or PVP or mexidol solutions. NPs size distribution in the liquid phase of the systems was determined by photon correlation spectroscopy, iron (Fe) concentration was evaluated by atomic emission spectrometry. It is shown that in the dispersion prepared in distilled water, the major amount of NPs was of 13-120 nm in size, in mexidol solution - 270-1700 nm, in PVP solution - 30-900 nm. In the fluid containing magnetite NPs together with mexidol and PVP, the main fraction (99.9%) was characterized by the NPs size of 14-75 nm with maximum of 25 nm. This system had the highest iron concentration: it was similar to that in the sample with mexidol solution and 6.6-7.3 times higher than the concentration in the samples with distilled water or PVP. Thus, in the preparation of aqueous dispersions based on magnetite NPs condensate, mexidol provides a transition of Fe to the liquid phase in amount necessary to achieve its biological activity, and PVP stabilizes such modified NPs.

PubMed | Karolinska Institutet, Regional Health Service Lazio Region, Libra Foundation, University of Plymouth and 212 more.
Type: | Journal: Clinical and translational allergy | Year: 2017

The Allergic Rhinitis and its Impact on Asthma (ARIA) initiative commenced during a World Health Organization workshop in 1999. The initial goals were (1) to propose a new allergic rhinitis classification, (2) to promote the concept of multi-morbidity in asthma and rhinitis and (3) to develop guidelines with all stakeholders that could be used globally for all countries and populations. ARIA-disseminated and implemented in over 70 countries globally-is now focusing on the implementation of emerging technologies for individualized and predictive medicine. MASK [MACVIA (

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