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Hradec Králové, Czech Republic

Friedecky B.,UKBD LF UK a FN | Kratochvila J.,SEKK Pardubice
Klinicka Biochemie a Metabolismus | Year: 2010

In near future we can expect some significant changes in performance of external quality assessment programs. These changes are conditioned namely by two factors. Firstly, by introducing the new standard ISO 17043 into practice use, and secondly by the change of quality control from classical control to tool of patients risk management. What does it all means for performance of EQA? Introducing the preanalytical and postanalytical as part of EQA programs. Intensification of their educational potential is expected. Much larger application of two essential parts of metrology-traceability and uncertainty is involved in EQA programs. Expected changes in EQA should begin by auditing respectively corrections of target values and particularly by control limits values.

Chmelarova M.,UKBD LF UK a FN | Palicka V.,UKBD LF UK a FN
Klinicka Biochemie a Metabolismus | Year: 2010

Objective: To review epigenetic changes in cancer cells focus on DNA methylation. Refer to perspective use of recent knowledge for early detection of ovarian cancer, which remains the important gynaecological problem. It is due to difficult diagnostics in early stages of ovarian cancer. Design: Review article. Setting: Institute for Clinical Biochemistry and Diagnostics Medical faculty and University hospital Hradec Králové. Conclusion: The research of cancer-specific DNA methylation changes represents an exciting new era in ovarian cancer diagnostics.

Vasatova M.,UKBD LF UK a FN | Tichy M.,UKBD LF UK a FN | Vavrova J.,UKBD LF UK a FN
Klinicka Biochemie a Metabolismus | Year: 2010

The publication reports recent approach to biochemical analyses. There are summarized data about principles, usages, advantages or disadvantages of new multiplex technologies. Although, in the meantime, the most of presented methods are in development or they are used in research projects some technologies already expand to clinical biochemistry laboratories.

Vasatova M.,UKBD LF UK a FN | Holeckova M.,UKBD LF UK a FN | Bartoskova I.,UKBD LF UK a FN | Tichy M.,UKBD LF UK a FN | Friedecky B.,UKBD LF UK a FN
Klinicka Biochemie a Metabolismus | Year: 2010

Background: The aim of our study was to compare concentration of troponin T measured by classic and high-sensitivity methods. Methods: Elecsys 2010 and E170 Modular system was used to assess the serum levels of cTnT and hs-cTnT (methods: Elecsys Tropo T hs, Elecsys Tropo T, Elecsys Tropo T hs STAT and Elecsys Tropo T STAT) (Roche). Cardiac troponin T was measured in 72 serum samples simultaneously. Bland-Altman analysis and Passing-Bablok regression were used for statistic interpretation. Numbers of results in relation to limit of detection and decision limits were compared. Results: In low concentration level (< 50 ng/l), hs-cTnT were higher in comparison with cTnT about 20-100%. Results of hs-cTnT measured by Elecsys 2010 and E170 Modular systems were comparable. Higher number of results above decision limit in hs-cTnT method means significant change of the diagnostic classification in comparison with fourth generation of cTnT method. Conclusions: Results of low troponin T concentration (< 30 ng/l) can be useful for clinical classification, but methods hs-cTnT and cTnT were not comparable in low concentration levels. Our data indicate existence of differences in calibrations of methods. It is necessary to define data relationship to reference materials more precisely, respectively to make an effort to their creation. With respect to clear trend increase of analytic sensitivity of cTnT measurement, only more sensitive hs-cTnT method should be commercially available.

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