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Hradec Králové, Czech Republic

Friedecky B.,UKBD | Friedecky B.,R.O.S.A. | Kratochvila J.,R.O.S.A.
Klinicka Biochemie a Metabolismus | Year: 2015

Objective: Comment of bias values reached in recent results of four commutable reference materials and programs in general blood serum analytes and their comparison with results of EQA program SEKK-AKS in 2012-2014. Methods: As commutable reference materials were used sets of individual blood donors and non spiked and spiked frozen native serum pools. Bias values were calculated as differences of manufacturer groups means from robust mean of all participants. In some cases were used values obtained by reference methods. Results, discussion: Majority of bias values did not exceeded 4 %. No significant dependency on the on the kind of matrix was observed. Some large bias values were observed only exceptionally in some methods of uric acid and phosphate. Measurement of albumin was influenced by differences between BCG and BCP methods, higher concentration of triglycerides is reason of large bias in HDL and LDL method. Commonly are bias values higher than requested, from biological variations derived. No significant matrix influence on the bias value was observed in measurement of general blood serum analyzed. We think that ex post commutability evaluation of EQA program results based on the use standard lyophilized materials can be realized by assessment relation of bias to uncertainty of reference values. There is necessary to prefer reference method values or robust mean values as criterion not only for proficiency of participants and also for monitoring of level in standardization. © 2015 Klinická biochemie a metabolismus.

Solcova L.,Oddeleni klinicke biochemie | Maisnar V.,II. Interni Klinika | Tichy M.,UKBD
Klinicka Biochemie a Metabolismus | Year: 2010

Multiple myeloma is the second most common form of haematological malignancy. The objective of this message are two casuistries related to the late diagnostics of multiple myeloma by female patients with osteoporosis and renal insufficiency. The goal is to draw attention to the group of patients at the late diagnosis risk (elderly women).

Free monoclonal immunoglobulin light chains (FLC) quantification is recommended for diagnosis assessment and monitoring therapy for patients with monoclonal gammopathy. But there are numerous uncertainties regarding the detection, interpretation and FLC quantification. Our interlaboratory study showed that kappa/lambda ratio is strongly influenced by measurement errors and therefore we recommended the preferential use of FLC concentration values. Unified protocols are needed to minimise interlaboratory variability introduced by manual dilution or volume augmentation of clinical sample.

Vasatova M.,UKBD | Tichy M.,UKBD | Pudil R.,I. interni klinika | Horakova L.,I. interni klinika | And 2 more authors.
Klinicka Biochemie a Metabolismus | Year: 2010

Background: The aim of our study was to monitor analytical and clinical performance of new high-sensitivity troponin T method (hs-cTnT). Methods: Elecsys 2010 system (Roche) was used to assess the serum levels of hs-cTnT. Cardiac troponin T (cTnT) was measured in populations: 73 healthy blood donors (BD), 41 acute myocardial infarction (AIM), 34 unstable angina pectoris (UAP), 38 stable angina pectoris (SAP), 22 hypertrophic cardiomyopathy (HKM) and 27 different types of arrhythmia (AR). Results: Concentration hs-cTnT was elevated above 99th percentile of reference population (BD) in 100 % AIM, 63.6 % UAP, 15.8 % SAP, 18.5 % AR and 13.6 % HKM. Conclusions: High-sensitivity method has adequate analytical performance. Our data indicate increase of hs-cTnT that can be monitoring of minor myocardial injury in AKS patients or chronic cardiac diseases. It is significant differences between BD, SAP and UAP results. This fact can be used for risk stratification. Results of low cTnT concentration (< 0.03μg/l) can be useful for clinical classification but it is necessary to establish reference limits.

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