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Granada, Spain

Iswaldi I.,University of Granada | Iswaldi I.,Functional Food Research and Development Center | Arraez-Roman D.,University of Granada | Arraez-Roman D.,Functional Food Research and Development Center | And 9 more authors.
Food and Chemical Toxicology | Year: 2013

As the beneficial effects of American cranberry (Vaccinium macrocarpon) can be partly attributed to its phenolic composition, the evaluation of the physiological behaviour of this fraction is crucial. A rapid and sensitive method by ultra-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been used to identify phenolic metabolites in human urine after a single dose of cranberry syrup. Prior to the analysis, metabolites were extracted using an optimised solid-phase extraction procedure. All possible metabolites were investigated based on retention time, accurate mass data and isotope and fragmentation patterns. Free coumaroyl hexose (isomer 1 and 2), dihydroxybenzoic acid, caffeoyl glucose, dihydroferulic acid 4-O-β-d-glucuronide, methoxyquercetin 3-O-galactoside, scopoletin, myricetin and quercetin, together with other 23 phase-I and phase-II metabolites, including various isomers, could be tentatively identified in the urine. Afterwards, the metabolites were simultaneously screened in the urine of different subjects at 0, 2, 4, and 6h after the ingestion of cranberry syrup by Target AnalysisTM software. © 2013 Elsevier Ltd. Source


Albanil Ballesteros M.R.,CS Cuzco | Calvo C.,Hospital Universitario SeveroOchoa | Hernando Helguero P.,CS Ramon y Cajal | Martinez Campos L.,UGC Pediatria | Dominguez Aurrecechea B.,CS de Otero
Pediatria de Atencion Primaria | Year: 2014

Introduction: sinusitis is a common condition whose approach in daily pediatric practice is not well known. Methods: performing a national level cross-sectional study through a survey distributed "on line" to members of 2 pediatric societies, in March 2013. Results: 994 responses were obtained (78% of Primary Care). Most professionals estimated the frequency of sinusitis being about 1% of the visits. 76.6% reported exclusively diagnosis with clinical criteria, considering (92%) an association of symptoms including persistent / purulent rhinorrhea (89%), facial pain / headache (84%), prolonged or nocturnal cough and fever in the context of upper respiratory infection (URI) (54%). Between 19.7 and 33.4% of professionals (primary / specialist) would request radiographs. 90% reported using antibiotics with or without other adjunctive treatments, being amoxicillin-clavulanate (52%) the chosen antibiotic, followed by amoxicillin 46%.81% of pediatricians diagnose URI in more than 30% of visits and in 85% of cases do not prescribe drug treatment. When the URI lasts more than 14 days, 70% of them decide to treat. The most widely used treatments are anti-inflammatory / analgesics (54.5%) and antibiotics (51.4%). Conclusions: pediatricians' knowledge and clinical practice in URI and sinusitis indicate a high level of compliance with the most recent clinical practice guidelines, highlighting the limited use of antibiotics in the URI, the clinical diagnosis of sinusitis and the empirical choice of antibiotic. Source


Rodriguez-Perez C.,University of Granada | Rodriguez-Perez C.,Research and Development Functional Food Center | Quirantes-Pine R.,Research and Development Functional Food Center | Contreras M.D.M.,University of Granada | And 6 more authors.
Food Chemistry | Year: 2015

Shelf life of commercial cranberry syrup irradiated with gamma radiation at a rate of 5 kGy and stored for 6 months at 25 °C and 60% relative humidity (RH) and under accelerated stability conditions was investigated. High-performance liquid chromatography coupled to electrospray ionisation quadrupole-time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS) was used to characterise cranberry syrup. Afterwards, these compounds were quantified by HPLC-ESI-QTOF-MS and 4-dimethylaminocinnamaldehyde (DMAC) assay. A significant increase in the content of procyanidin B isomer 1 (from 4.4 to 7.0 μg/ml) and procyanidin A2 (from 83 to 93 μg/ml) was observed after irradiation and compared with the non-irradiated syrup. Procyanidin B isomers and prodelphinidin were stable at 25 °C during the first month of storage, whereas quercetin and some derivatives remained constant for 3 months of storage at this temperature. In short, after gamma-irradiation in dose of 5 kGy, most compounds were highly stable for a month at 25 °C. © 2014 Elsevier Ltd. All rights reserved. Source


Ocete Hita E.,Hospital Universitario Virgen Of Las Nieves | Martin Garcia J.A.,Hospital Universitario Virgen Of Las Nieves | Gimenez Sanchez F.,UGC Pediatria | Flores Gonzalez J.C.,Hospital Universitario Puerta del Mar | And 3 more authors.
Anales de Pediatria | Year: 2013

Introduction: The incidence of adverse drug reactions in children has recently been established at 15.1 reactions per 1000 children. This represents 2% of admissions to a paediatric hospital, and is similar to adult patients. Only a small percentage (less than 8%) may have liver involvement, which can range from a slight increase in transaminases to fulminant hepatitis. The aim of this study was to determine the importance (frequency, types of presentation, severity and chronicity) of hepatotoxicity by drugs or natural remedies in the paediatric population. Patients and method: All paediatric patients, neonates and children who had suspected hepatotoxic reactions notified by the eight participating Spanish hospitals. The Council for International Organizations of Medical Sciences (CIOMS) scale was used for the analysis of causality in each case. Results and conclusions: We studied a total of 36 hepatotoxic reactions in 33 children. The drug classes most frequently involved were antimicrobials (71%). Amoxicillin-clavulanate was the individual drug responsible for the greatest number of cases (31.4%). We conclude that the registration of drugs associated with liver disease has proved a useful tool for creating an active network of motivated specialists in detecting and reporting incidents of toxic liver disease, ensuring increasing diagnostic accuracy. © 2012 Asociación Española de Pediatría. Published by Elsevier España, S.L. All rights reserved. Source


Molina-Carballo A.,Servicio de Neuropediatria | Molina-Carballo A.,University of Granada | Justicia-Martinez F.,Servicio de Neuropediatria | Moreno-Madrid F.,UGC Pediatria | And 10 more authors.
Psychopharmacology | Year: 2014

Rationale: Attention deficit with hyperactivity disorder is a neurodevelopmental disorder associated with alterations in the prefrontal cortex via dopaminergic and noradrenergic neurotransmission. Neurosteroids (e.g. allopregnanolone and dehydroepiandrosterone) modulate the release of multiple neurotransmitters. Objective: This study aims to determine the baseline concentrations and daily variations in allopregnanolone and dehydroepiandrosterone in children with attention deficit hyperactivity disorder (ADHD) and to determine the effect of chronic administration of methylphenidate on clinical symptoms and on the concentrations of these two neurosteroids. Methods: We included 148 children aged 5 to 14 years, subdivided into two groups: ADHD group (n = 107, with a diagnosis of ADHD (DSM-IV-TR criteria), further classified in subtypes by an "attention deficit and hyperactivity scale" and subgroups by the "Children's Depression Inventory") and a control group (n = 41). The clinical workup included blood samples that were drawn at 20:00 and 09:00 hours, at inclusion in both groups, and after 4.61 ± 2.29 months of treatment only in the ADHD group, for measurements for allopregnanolone and dehydroepiandrosterone. Factorial analysis, adjusted for age and gender, was performed by using Stata 12.0. Results: Methylphenidate induced the doubling of allopregnanolone levels in the predominantly inattentive ADHD patients without depressive symptoms (27.26 ± 12.90 vs. 12.67 ± 6.22 ng/ml, morning values). Although without statistical differences, baseline dehydroepiandrosterone levels were higher and slightly increased after methylphenidate in the ADHD subtype with depressive symptoms (7.74 ± 11.46 vs. 6.18 ± 5.99 ng/ml, in the morning), opposite to the lower baseline levels, and further decrease after methylphenidate in the inattentive subtype with depressive symptoms. Conclusions: Different neurosteroids may have different baseline concentrations and differential responses to methylphenidate treatment as a function of ADHD subtype and subgroup. These differential responses may be a clinical marker of ADHD subtype and/or co-morbidities. © 2014 Springer-Verlag. Source

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