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This experiment evaluated the effects of temperature on sex inversion survival and presence of deformities in larvae of tilapia subjected to the following treatments control (28°C + diet 60 mg kg-1 of 17 α-methyltestosterone), 28, 30, 32 and 34°C. The results showed a statistical difference between the proportion of males, with temperatures control, 32 and 34°C (98, 91, 96%, respectively) showed better results than those obtained for treatments 28 and 30°C (71 and 82%). The survival observed in different treatments were 90.74, 90.49, 88.20, 85.43 and 82.16 for the control, 28, 30, 32 and 34°C, respectively, and there was statistical difference between treatments 32 and 34 °C, only when these were compared with the control. In the analysis of the radiographs was not possible to observe the presence of morphological changes in fish subjected to different treatments. With this experiment, we concluded that the temperatures of 32 and 34C were able to genetically larvae masculinizing females, and that there was no increase in mortality and the presence of deformities could be attributed to treatment. This technique is an alternative to use of steroid hormones.


Zanoni M.A.,University of Northern Paraná | Costa F.G.,University of Northern Paraná | Nazareno A.,UENP | Oliveira C.A.L.,State University of Maringá | Ribeiro R.P.,State University of Maringá
Semina:Ciencias Agrarias | Year: 2014

The experiment was conducted to evaluate the influence of tamoxifen on sexual inversion of Nile tilapia larvae from two days of life, the experimental desing was compounded of five treatments and three replicates, and fish were fed diets with different amounts of tamoxifen: 0, 25, 50, 75 and 100 mg kg -1 of feed. After 28 days of treatment, larvae were counted and transferred to water tanks of 500 liters and then which were fed with a diet containing 28% crude protein for more 60 days. After this period the fry were sexed by the "squash". The survival 75.5 ± 5.74, 73 ± 4.76, 66 ± 11.19, 76.5 ± 4.12, 75 ± 3.46 and average percentage of male individuals 62.5 ± 5, 70 ± 8.1, 60 ± 8.2, 70 ± 0.0, 62.5 ± 9.5 for treatments 0, 25, 50, 75 and 100 mg of tamoxifenkg-1 diet, respectively, showed no statistically significant differences (p<0,05). The number of gonads in each fish experienced a significant reduction in the treatments with 75 and 100 mg of tamoxifen (89.3 and 79.3 ± 0.527 ± 0.378) when compared with treatments 0, 25 and 50 mg of tamoxifen (98, 9 ± 1.0, 97.6 ± 1.0 and 94.5 ± 1.0, respectively), the histological structure of female gonads did not differ and ovaries in all treatments showed well-defined and oocytes at several stages of maturation.


Gandolfo M.A.,UENP | Carvalho F.K.,São Paulo State University | Chechetto R.G.,São Paulo State University | Gandolfo U.D.,São Paulo State University | De Moraes E.D.,UENP
Engenharia Agricola | Year: 2014

Each year, there is an increase in pesticide consumption and in its importance of use in the large-scale agricultural production, being fundamental the knowledge of application technology to the activity success. The objective of the present study was to evaluate the influence of working pressure on the drift generated by different spray nozzles, assessed in wind tunnel. The treatments were composed of two spray nozzles AXI 110015 and AXI 11002 with pressure levels of 276 and 414 kPa. The spray solution was composed by water and NaCl at 10%. The applications were conducted at wind speed of 2.0 m s-1, being the drift collected at 5.0; 10.0 and 15.0 m away from the spray boom and at heights of 0.2; 0.4; 0.6; 0.8 e 1.0 m from the tunnel floor. To both spray nozzles, the greatest drift was collected at the smallest distance to the spray-boom and at the lowest height. The AXI 11002 nozzle gave a smaller drift relative to the AXI 110015 nozzle for the two tested pressures and for all the collection points. Regardless of the nozzle, a rise in the working pressure increases the spray drift percentage at all distances in the wind tunnel.


Today the brazilian poultry industry sets Brazil as the third largest producer of chicken meat. The poultry production sector is extremely organized in all its segments, from birth to slaughter poultry. One of the most laborious is the pre-slaughter, which ranges from the feed withdrawal to the lairage time at the plant. Due to this fact this research highlights the most critical points in the pre-slaughter period, as: feed withdrawal, capture, catching, transport and lairage time in the plant.


Do Prado Firmino M.R.,University of Northern Paraná | De Oliveira Araujo L.,UENP | Sanches Bermudes J.P.,UENP | Moreira R.C.,UENP | And 2 more authors.
Gerokomos | Year: 2013

Introduction: the lack of epidemiological data about leg ulcers in Brazil justifies this study. So, the aim of that research was to estimate the prevalence of leg ulcers. Methods: epidemiological prevalence study, by conglomerates, performed at Bandeirantes city - PR, Brazil, between August-November 2012. Results: 1,499 individuals participated on the study. Prevalence was 6/1,000 persons, with major occurrence between women and elder people. Discussion: Prevalence found was higher than other studies like those preformed in Spain and Portugal, and the profile of the people was very similar to literature.


In order to increase accuracy and reduce the subjectivity in the evaluation of radiographies, several methods have emerged. The most accepted of these methods is the Vertebral Heart Size method (VHS), or the heart's size versus the thoracic vertebra's unit. The intention of this research was to apply the VHS measurement method in clinically healthy American pit bull terrier, with the purpose of establishing the average VHS for this breed. Chest x-rays in lateral projection were taken, and these were empirically evaluated and measured so that a VHS value was obtained, as well as the thoracic depth/ width (D/W) relation for each animal. None of the animals had abnormal results when evaluated via empiric analysis. VHS values had a normal distribution, as did D/W relations, with an average of 10.9±0,4 vertebrae for VHS and 0.80±0,07 for D/ W relations. VHS values showed a significant difference (P>0.05) when compared to the results obtained by BUCHANAN & BÜCHELER (1995). However, VHS values in this study are similar to those described by several other authors, confirming the need to establish values of VHS for each breed.


Gandolfo M.A.,UENP | Antuniassi U.R.,São Paulo State University | Gandolfo U.D.,São Paulo State University | De Moraes E.D.,University of Northern Paraná | And 2 more authors.
Engenharia Agricola | Year: 2013

To optimize the use of pesticides, several countries have carried out periodic inspections in agricultural sprayers. In Brazil, knowing the conditions of this machinery can guide researches and investments in guidelines for its use and maintenance. The objective of this study was to verify the state of sprayer maintenance used in the North of the state of Paraná, in Brazil. Several sprayer items were evaluated, such as: presence, status and scale of the manometer, status of the hose, status of the anti-drip component, presence of leaks, status of the bar, status of the filters, state of the spraying nozzles and errors in the targeted flow rate. Machines were named as approved when there was no failure in any item evaluated. The factor that caused the biggest level of reprove among the machines was incorrect scale of manometers, which reproved 84.55% of the machines evaluated. Other outstanding factor was the incorrect flow rate in 75.5% of the tested machines. Only one unit was approved from the total of 110 evaluated sprayers.

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