Campos, Brazil
Campos, Brazil

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Oliveira R.D.S.,UENF CCTA LFIT | Pereira M.R.,UENF CCTA LFIT | Carvalho V.S.,UENF CCTA LFIT | Da Silva J.R.,UENF CCTA LMGV | Campostrini E.,UENF CCTA LMGV
Revista Brasileira de Fruticultura | Year: 2015

The objective of this study was to evaluate the efficiency of chemical sterilization of the culture medium and alternative gelling agents and their influence on development in vitro and ex vitro plants of pineapple ‘Vitória’. The culture medium used was composed of MS salts and White vitamins, with 30 g L-1 of sucrose and 100 mg L-1 of myo-inositol. Two forms of sterilization of the media culture and the glassware were performed: in autoclave at 121°C and with solution of sodium hypochlorite (NaClO) at 0.003% to rinse the glassware and 0.0003% in the culture medium. The gelling agents tested were: M1: agar (6.0 g L-1); M2: corn starch (60.0 g L-1); M3: agar (3.0 g L-1) + corn starch (30.0 g L-1) and M4: agar (3.0 g L-1) + cassava starch (30.0 g L-1). After 30 days of in vitro cultivation was found that the NaClO and alternative gelling agents did not affect the growth and development of plantlets. Part of the plantlets of each treatment was acclimatized during 90 days, after which it was found that there was no influence of gelling agents on plantlets growth. For most of the parameters evaluated, the plantlets derived from treatments involving the chemical sterilization showed results similar or superior to the autoclaved media. © 2015, Sociedade Brasileira de Fruticultura. All rights reserved.


dos Santos P.C.,UENF CCTA LFIT | Nogueira A.S.,EAFSI | Freitas M.S.M.,UENF CCTA LFIT | Freitas J.A.A.,UENF CCTA LFIT | de Carvalho A.J.C.,UENF CCTA LFIT
Revista Brasileira de Fruticultura | Year: 2014

The lack of technologies that allow improvements in sugar-apple culture management, mainly regarding to pruning time and pollination methods, restricts the growth of the cultivated area with this fruit in Brazil. In this sense, the aim is of evaluating sugar-apple fruit development and growth on the basis of pruning time and pollination methods. The experiment was conducted in the city of São Francisco do Itabapoana-RJ, in the period from April 2001 to February 2002. The experimental design was randomized blocks design (RBD), with plots subdivided in 5×3 factorial scheme, being five times of pruning (May, June, July, August and September 2001) and three pollination methods (natural pollination; pollination with soft brush, held directly in flower and pollination with pump), with four replications. The length and diameter of the branches were influenced by the pruning season. Both artificial pollination methods increased the percentage of fruit fertilization. Flowering was influenced by pruning time. On average, the peak of fruit development was verified about 50 days after pollination and it was higher when artificial pollination was used.


de Oliveira M.T.R.,Tecnologa em Horticultura | Berbert P.A.,UENF CCTA LEAG | Carlesso V.O.,UENF CCTA LFIT | Thiebaut J.T.L.,UENF CCTA LFIT | And 2 more authors.
Revista Brasileira de Sementes | Year: 2011

Information on the physiological potential of carambola seeds is practically inexistent although interest in carambola production has been increasing steadily over recent years. The objective of this study was to investigate the immediate effect of convective drying at 38°C on seed germination and vigour. Germination tests under controlled laboratory conditions were done using rolled paper towels, whereas tests in a protected environment were done using a lightweight, commercial seed-starting medium. In a protected environment, convective drying increased the percentage germination from 74% (fresh seeds) to 98% (dried seeds). Vigour, as estimated from the germination speed index and the mean time of germination, did not vary, irrespective of drying. The curves representing plant growth rate and the dry mass accumulation rate of the aerial parts of the plant, show that seeds which had been dried gave rise to more vigorous plants.


dos Alves F.S.,UENF CCTA LFIT | Jasmim J.M.,UENF CCTA LFIT | de Carvalho A.J.C.,UENF CCTA LFIT | Thiebaut J.T.L.,UENF LEAG
Horticultura Brasileira | Year: 2010

It was evaluated the quality and nutrient content of lady palm in four substrates made up of a mixture of shredded coconut fiber (FC) and commercial substrate Plantmax HT® (SC), in proportions of 0, 25, 50 and 75% of FC, in 35 liters containers, in a randomized block experiment with four replicates and three pots per plot, with one plant per pot. The plants were fertilized every three months alternating 25 g of 4-14-8 with 10 g of urea per pot, at planting and throughout the 24 months of the experimental period. Growth was evaluated by measuring the height of the initial stem (from the stem base to leaf insertion of the most recently expanded leaf), number of leaves in the original stem, number of offsets, number of leaves in offsets, offset height and nutrient content of last completely expanded leaf in the initial stem. The quality was evaluated by visual sensorial analysis using the criteria "I liked it" (a lot or a little) and "I would buy it" (yes or no). There was no significant difference on the growth characteristics among treatments, except on the number of leaves of the initial stem. Plants with the best acceptance were those grown in the mixture 50% FC and 50% SC. None of the plants showed visual nutrient deficiency symptoms; the leaf nutrient content of healthy plants with market quality standards were: 17.80 - 18.29 g kg-1 of N; 0.66 - 0.81 g kg-1 of NO-3; 2.02 - 2.34 g kg-1 of P; 18.11 - 20.40 g kg-1 of K; 11.63 - 13.84 g kg-1 of Cl; 2.69 - 4.04 g kg-1 of Ca; 1.61 - 2.07 g kg-1 of S; 1.98 - 2.64 g kg-1 of Mg.


Pereira M.R.,UENF CCTA LFIT | Carvalho V.S.,UENF CCTA LFIT | de Lucas E.F.,UENF CCTA LFIT | de Gravina G.A.,UENF CCTA LFIT
Revista Brasileira de Fruticultura | Year: 2015

This study aimed to reduce the production cost of culture media for agar substitution by corn starch and physical sterilization in autoclave by chemical sterilization with sodium hypochlorite (NaClO) in the plantlets of pineapple ‘Vitória’ propagated in vitro. The shoots were transferred to rooting medium composed of MS salts, White´s vitamins, myo-inositol and sucrose. The experiment was conducted in a completely randomized design in a factorial 3 x 2: media jellified with agar (6,0 g L-1), corn starch (60,0 g L-1) and agar (3,0 g L-1) + corn starch (30,0 g L-1), sterilized in autoclave or chemically boiling the medium and use of 0,05% NaClO to rinse the glassware. After a month of in vitro rooting, part of the plants were evaluated for number of leaves and roots and masses of fresh and dry materials. The rest of the plantlets was acclimatized for 90 days in a greenhouse. At the end of this period number of leaves, number of roots, fresh weight and dry weight of aerial part, root and total; leaf area, height and diameter of rosette were evaluated. The boiling + chemical sterilization did not differ of autoclave sterilization for the majority of parameters, while the corn starch enhanced growth of plantlets both in vitro and during acclimatization. © 2015, Sociedade Brasileira de Fruticultura. All rights reserved.


Da Silva Oliveira R.,UENF CCTA LFIT | Pereira M.R.,UENF CCTA LFIT | Carvalho V.S.,UENF CCTA LFIT | De Fatima Lucas E.,UENF CCTA LFIT | De Amaral Gravina G.,UENF CCTA LFIT
Revista Brasileira de Fruticultura | Year: 2015

This study aimed to evaluate the total or partial replacement of agar by corn starch and cassava starch and physical sterilization in autoclave for chemical sterilization with sodium hypochlorite (NaClO) in development of pineapple ‘Gold’ plantlets. The gelling agents used were: M1: agar (6.0 g L-1); M2: corn starch (60.0 g L-1); M3: agar (3.0 g L-1) + corn starch (30.0 g L-1) and M4: agar (3.0 g L-1) + cassava starch (30.0 g L-1). The sterilizations were made by autoclaving at 121°C or by using the solutions of NaClO at the concentrations of 0,003% to rinse the glassware and 0,0003% for addition to the culture medium. After one month of in vitro cultivation, it was observed no negative influence of NaClO and gelling agents in the rooting in vitro of shoots for most parameters evaluated. The plantlets derived from in vitro phase were taken to acclimatization in greenhouse for 90 days. During the acclimatization, the plantlets derived from treatments with chemical sterilization did not differ from the ones derived of autoclaved media to any parameter evaluated, whereas the combination agar + corn starch provided inferior results among gelling agents. © 2015, Sociedade Brasileira de Fruticultura. All rights reserved.


de Almeida L.V.B.,UENF CCTA LFIT | Marinho C.S.,UENF CCTA LFIT | Muniz R.A.,UENF CCTA LFIT | de Carvalho A.J.C.,UENF CCTA LFIT
Revista Brasileira de Fruticultura | Year: 2012

The slow release fertilizers are used to reduce the frequency of fertilization because provides gradually the nutrients to the plants. However the release of nutrients should be the same that nutrient demand by plants. The aim of this study was to evaluate availability of N, P and K and growth of two citrus rootstocks when slow release and conventional fertilizers were applied. Two fertilizations systems in a commercial substrate were appraised (with controlled-release fertilizer and with only conventional fertilizers), two rootstock ('Rangpur lime' tree and tangerine 'Sunki' tree) and five sampling times (30, 60, 90, 120 and 180 days after the transplanting) with five repetitions and two plants per plot. The uses of slow release fertilizer increase stem diameter, leaves number, P contents in leaves of rootstocks and P and K disponibility in substrate, when compared with use of conventional fertilization.


Santos P.C.D.,UENF CCTA LFIT | de Freitas S.J.,UENF CCTA LFIT | Freitas M.S.M.,UENF CCTA LSOL | de Sousa L.B.,UENF CCTA LFIT | de Carvalho A.J.C.,UENF CCTA LFIT
Revista Brasileira de Fruticultura | Year: 2011

One of the main obstacles to the development of pineapple cultivation in Brazil has been the absence of seedlings in both quantity and quality, for propagation. Among the alternatives, the obtaining of seedlings is verified starting from the sprouting from crowns of fruits, which are normally discarded by the consumer. Furthermore, the use of arbuscular mycorrhizal fungi (AMF) can be an alternative for improving the production of seedlings, since these fungi can shorten the time of seedlings of various fruit. In that sense, the objective of this study was to evaluate the production of seedlings type suckers, using the method of destruction of the apical meristem of the crown of the pineapple plants inoculated with FMAs. For such, it was used the randomized complete block design, factorial 3x3, with three cultivars of pineapple ('Smooth Cayenne', 'Pérola' and 'Jupi') and three microbiological treatments (no inoculation, Glomus etunicatum and a mixture of Glomus clarum and Gigaspora margarita), with four replications. The first emissions were recorded at 30, 60 and 90 days after planting for cultivar Smooth, 'Pérola' and 'Jupi', respectively. The pineapple 'Smooth Cayenne' produced 80 to 69% seedlings more than the cultivars 'Pérola' and 'Jupí', respectively. In microbiological treatments there was a significant difference for the suckers issued, in which the mixed treatment was the most prevalent issue when compared with treatment with G. etunicatum. Nutritional assessments in the crowns, inoculation with the mixed treatment promoted an increase of 85 and 66% for P, 22 and 13% for N and 6 and 19% for K, compared to treatments G. etunicatum and control respectively. It is concluded that the production of suckers from crown whose principal gem was beheaded is an alternative for the production of pineapple seedlings, being more efficient in cultivar 'Smooth Cayenne' producing 26 seedlings at 420 days after planting.

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