Leiva-Revilla J.,State University of Ceará |
De Los Reyes Cadenas J.,State University of Ceará |
Vieira L.A.,State University of Ceará |
Campello C.C.,UECE |
And 6 more authors.
Semina:Ciencias Agrarias | Year: 2017
The extract of Auxemma oncocalyx (A. oncocalyx) and its main component, oncocalyxone A (onco A), possess anti-Tumoral activity that may affect fertility. There is limited literature on the action of these substances regarding caprine folliculogenesis. In this study, we evaluated the effect of A. oncocaly and onco A on the in vitro culture of isolated secondary follicles (Experiment 1) and on the in vitro maturation (IVM) of oocytes from caprine antral follicles grown in vivo (Experiment 2). Isolated secondary follicles were distributed in six groups: The non-cultured control was immediately fixed in 4% paraformaldehyde. The remaining follicles were cultured for 7 days in α-minimal essential medium (α-MEM+) alone (control) or with dimethyl sulfoxide (DMSO), doxorubicin (DXR), A. oncocalyx, or onco A. After culture, the follicles were evaluated for antrum formation, growth rate, apoptosis (TUNEL), cellular proliferation (PCNA), as well as the expression of BCL2 and BAX. In addition, cumulus oocyte complexes (COCs) were aspirated and allocated into five treatments for IVM: control, cultured only in maturation base medium (TCM 199+); or supplemented with DMSO; DXR; A. oncocalyx or onco A. After IVM, oocyte chromatin configuration and viability were assessed. After 7 days of culture, a reduction was noted in the percent of morphologically intact follicles, antrum formation, growth rate, and numbers of PCNA-positive granulosa cells (P < 0.05). After culture, the DXR treatment showed a higher percent of TUNEL-positive follicles and relative BAX:BCL2 mRNA ratio's (P < 0.05). After IVM of the COCs, DXR, A. oncocalyx, and onco A treatments showed a greater percent (P < 0.05) of abnormal oocytes and a lower percent of viable oocytes as compared with the control group (P < 0.05). However, only DXR and onco A treatments increased the percent of alive oocytes with abnormal chromatin configuration (P < 0.05). There were no differences in the maturation rates among the control group and DXR, A. oncocalyx, and onco A treatments. In conclusion, under our culture conditions, A. oncocalyx and onco A did not exhibit toxic effect on isolated secondary follicles and on maturation rates of COCs recovered from antral follicles. However, these substances negatively affected the oocyte viability. Thus, culture methods including in vitro secondary follicle culture and in vitro oocyte maturation toxicity test are appropriate methods to evaluate the possible effects of drugs on folliculogenesis.
Aguiar-Silva F.H.,National Institute of Amazonian Research |
Bonilla O.H.,UECE |
Revista Caatinga | Year: 2011
We analyzed the viability and effectiveness of restricted-use protected areas aiming to identify gaps in the definition of priorities to protect wild regions from Ceará. Based on the creation of legal documents and interviews with managers of protected areas, we analyzed the size, we verified the existence of the management plan, monitoring activities and the criteria used in selecting the area. We found that in eight of the sixteen protected areas had not followed technical-scientific criteria, nor had carried through previous studies of the resources when they had chosen the areas to be transformed into completely protected areas. Fifteen do not possess management plans and 13 do not have environmental monitoring activities. Eleven protected areas have less than 10,000 ha, and insufficient studies that accurately ascertain the extent appropriate to maintain the biodiversity of the ecosystems of Ceará. It is necessary to deploy tools for maintaining these forests to protect the wildlife and the environmental services provided by these areas.
Soares L.E.S.,UERN |
Barbosa Silva K.M.,UERN |
Barbosa Z.,UECE |
Revista Caatinga | Year: 2011
The genus Lippia (Verbenaceae) comprises about 200 species of pantropical distribution. Lippia gracilis Schauer, popularly known as the mountain balm, is an aromatic plant, native of northeastern Brazil, used in folk medicine as an antiseptic and to treat dermatitis. Although cytogenetic serve as a tool in taxonomy rate, cytogenetical studies of genus Lippia are restricted. Given the above, the present study aimed to determine, at the first time, the chromosome number of copies of L. gracilis, and thereby contribute to a better taxonomic understanding of this genus. In this sense root tips collected from newly planted specimens were pretreated with a solution of 2mM 8-hydroxyquinoline for 4 hours, fixed in Carnoy 3:1 (ethanol: acetic acid) for 24 hours and stored in the same fixative for later analysis. The roots were hydrolyzed in 5N HCl for 20 minutes and the slides, prepared by squashing in acetic acid (45%) and stained with 2% Giemsa. The best metaphases were photographed and pointed to the chromosome number 2n = 24. Moreover, were observed metacentric and submetacentric chromosomes. Because there are several chromosome numbers in the genus, from n=10 to n=30, and as the same is little known cytogenetically, f more comprehensive cytogenetic studies are necessary, involving a larger number of species, so these data may help to clarify taxonomic inconsistencies in the family Verbenaceae.
Adriano L.S.,State University of Ceará |
Maia F.M.M.,UECE |
Carioca A.A.F.,University of Sao Paulo |
Nutricion Clinica y Dietetica Hospitalaria | Year: 2013
Objective: To evaluate the association of practicing alcoholic binge (BA) with the prevalence of metabolic syndrome (MetS). Methods: The study was conducted with 90 undergraduate nutrition students, in 2010. We analyzed glucose, triacylglycerol (TG), high density lipoprotein (HDL-C), blood pressure and waist circumference. To calculate the prevalence of MetS, we used the diagnostic criteria of the American Heart Association, 2009. To the binge practice was used criterion of the National Institute on Alcohol Abuse and Alcoholism, 2004. Results: The prevalence of MetS was 5.6% and 17.8% of BA. There was association between the practice of BA and MetS (p = 0.003). Conclusion: The practice of alcoholic binge is associated with metabolic syndrome in university.
Alvarado Alcocer J.C.,UNILAB |
Furlan Duarte J.B.,UNIFOR |
Cavalcanti Pereira M.,Brazil |
Moura De Oliveira M.L.,UECE |
And 3 more authors.
IEEE Latin America Transactions | Year: 2015
Biomass is a renewable energy source, its use has received great attention due to environmental considerations and the increased need for energy worldwide, is clean and emit low greenhouse gas emissions compared with fossil fuels. The bark of cashew nuts (CCC), waste from the processing of almonds, which is excessively generated in our state and has not been administered correctly, so it is wasting its full potential, i.e., the main source for generating energy from biomass is in the waste. However, the use of agro industrial wastes energy is obtained by performing the archaic form of direct burning in ovens and chairs. Through the biomass gasification, process which converts the waste gas with an energy properties. We studied the conditions for utilization of biomass in order to obtain the best thermochemical conversion of biomass (direct combustion, pyrolysis and gasification). Had the opportunity to know the full operation of the gasifier, the phases of operation (heating, drying, pyrolysis and gasification) and to influence and select the ideal operating conditions. Applying methods of thermodynamic analysis and energy found in the literature and using data collected from tests performed in the fluidized bed gasifier installed was possible to determine the efficiency and potential (energy available and dissipated) of the gasifier. © 2015 IEEE.
Teixeira R.S.C.,UECE |
Cardoso W.M.,UECE |
Lopes E.S.,UECE |
Rocha-e-Silva R.C.,UECE |
And 3 more authors.
Revista Brasileira de Ciencia Avicola | Year: 2013
The aim of this study was to investigate the presence of Salmonella in common quails submitted to forced molting. A total of 240 quails were divided at 40 weeks of age into four groups: CG (control, quails not submitted to molting); FM (fasting method); WM (fed wheat midds ad libitum); and ZM (zinc oxide method). From each group, 10 cloacal swabs, 10 fecal samples, and 20 egg samples were collected before molting (two weeks) and after molting (two weeks). The microbiological procedures for Salmonella spp. identification were performed in four steps. The agglutination test, using somatic and flagellar antigens, was used to confirm Salmonella-suspected colonies. According to the methodology applied, none of the samples was positive for Salmonella spp. The results showed that 20.0% of the egg samples from birds submitted to forced molting were contaminated with enterobacteria. It was concluded that, under the conditions of the present experiment, the stress caused by forced molting did not induce infection by Salmonella spp. or increased Enterobacteriaceae contamination levels in the eggs.
Alcocer J.C.A.,UNILAB en Acarape CE |
Duarte J.B.F.,UNIFOR en Avenida Washington Soares 1321 Fortaleza CE |
Pereira M.C.,UNIFOR y Actualmente Se Encuentra en |
De Olveira M.L.M.,UECE |
And 3 more authors.
Proceedings of the 2014 IEEE Central America and Panama Convention, CONCAPAN 2014 | Year: 2014
Biomass is a renewable energy source, its use has received great attention due to environmental considerations and the increased need for energy worldwide, is clean and emit low greenhouse gas emissions compared with fossil fuels. The bark of cashew nuts (CCC), waste from the processing of almonds, which is excessively generated in our state and has not been administered correctly, so it is wasting its full potential, ie, the main source for generating energy from biomass is in the waste. However, the use of agroindustrial wastes energy is obtained by performing the archaic form of direct burning in ovens and chairs. Through the biomass gasification, process which converts the waste gas with an energy properties. We studied the conditions for utilization of biomass in order to obtain the best thermochemical conversion of biomass (direct combustion, pyrolysis and gasification). Had the opportunity to know the full operation of the gasifier, the phases of operation (heating, drying, pyrolysis and gasification) and to influence and select the ideal operating conditions. Applying methods of thermodynamic analysis and energy found in the literature and using data collected from tests performed in the fluidized bed gasifier installed was possible to determine the efficiency and potential (energy available and dissipated) of the gasifier. © 2014 IEEE.
Cavalcante C.M.,Federal University of Ceará |
Jorge M.S.B.,UECE |
dos Santos D.C.M.,State University of Ceará
Physis | Year: 2012
The paper discusses the current challenges encountered in caring for children with mental health problem in the context of the Family Health Strategy (FHS). Therefore, this study sought to understand how the matrix support (MS) has been used as a tool for care of children with mental health problems in the Family Health Strategy. Interviews were conducted with professionals involved in the work of MS in mental health in the FHS and relatives of children seen by this form of mental health care. The narratives were analyzed by Paul Ricoeur's Hermeneutic- Phenomenology approach. When questioned about cases of child seen at matrix support, it was discussed, even with some controversy, almost no attention to this age group. Therefore, it was realized that not only the issue of child mental health is still poorly studied, but its population is not perceived in the context of the FHS. At the same time, the mother's difficulty in exposing the children's mental health problems in the context of the FHS has also inhibited the care for this population. Moreover, little training in child mental health of health workers has become an obstacle to the effectuation of care for children. Thus, these issues must be discussed and addressed effectively, so that, as recommended to adults, children with mental health problems can be cared for in a comprehensive way, being, however, observed differences in their development and needs.
Junior F.R.F.G.,Federal University of Paraiba |
de Freitas A.A.F.,UECE |
de Almeida Guimaraes L.G.,FMGR |
Primo M.A.M.,Federal University of Pernambuco |
Machado M.A.V.,Federal University of Paraiba
Producao | Year: 2013
The aim of the present research was to perform a discreet choice preference analysis model and assess the oilseeds offer, through Stated Preference data, considering that the Brazilian Law 11.097/2005 establishes compulsory introduction of biodiesel in the Brazilian energy matrix, guaranteeing its demand. Descriptive survey methodology was used. The universe of this study comprised median and small national producers, adding up to a sample of 114 valid answered questionnaires. Quantitative model with Stated Preference Analysis of data treatment was used. Among the main results of this study, it was possible to verify that the most important attributes were economic return and existence of satisfactory national technical assistance. The variable extracted oil content is presented as unimportant to national producers' choice. A suggestion for further studies is the application of similar researches to other Brazilian cities and States.
PubMed | State University of Ceará and UECE
Type: Journal Article | Journal: Revista gaucha de enfermagem | Year: 2015
The objective of this investigation was to identify how adolescents structure the social representations of sexual intercourse and use of condoms. Exploratory and descriptive research was conducted with a convenience sample consisting of 234 students of a public secondary school in Fortaleza-CE, Brazil, between July 2009 and April 2010. Data were collected using a questionnaire with variables on socioeconomic status and sexual behaviour, and Free Association test with three terms: sex, unprotected sex and sex with a condom. Sexual intercourse was represented by love and affection among the women, while men associated sex to pleasure, desire and attraction toward the female body. The condom was considered important by both groups, but men represented condoms as being something bad that restricts pleasure. Health professionals are granted the opportunity to identify vulnerabilities of this population to DST/HIV/AIDS and work with these vulnerabilities in the most appropriate way.