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Campo Grande, Brazil

Ramalho R.T.,Federal University of Mato Grosso do Sul | Aydos R.D.,Federal University of Mato Grosso | Cereda M.P.,UCDB
Acta Cirurgica Brasileira | Year: 2010

Purpose: To evaluate the antitumor effect of acetone cyanohydrin in Ehrlich ascites tumor cells in vitro. Methods: The Ehrlich ascites tumor cells and lymphocytes were incubated with different concentrations of acetone cyanohydrin (0, 0.5, 1.0, 2.0, 10.0, 20.0 and 30.0 μg.mL-1), After 1, 2, 3, 4, 18 and 24 hours cell viability tests were performed by the trypan blue method. Results: The results demonstrated a dose-dependent cytotoxic effect against the cells of Ehrlich ascites tumor. The concentrations of 20 and 30 μg.mL -1 was 100% of cell death in only 1 and 2 hours respectively. In lower doses of 0.5, 1.0 and 2.0 μg.mL-1 the cytotoxic effect was less intense, increasing gradually with time. Conclusions: At low concentrations of 0.5, 1.0 and 2.0 μg.mL-1, more than 90% of cell death was observed only after 24 hours of incubation which is the evidence that the tumor cell has the ability to poison cumulatively and irreversibly itself with the acetone cyanohydrin when compared with the results presented by human lymphocytes that the same doses and at the same time of incubation reached a maximum of 30% of cell death, suggesting an activity of rhodanese differentiated between the two cells. Source


dos Santos W.B.R.,IFAM | dos Santos G.T.,State University of Maringa | Neves C.A.,State University of Maringa | De Marchi F.E.,State University of Maringa | And 4 more authors.
Revista Brasileira de Zootecnia | Year: 2012

Four multiparous Holstein cows averaging 548 kg of body weight and 74 d in lactation were used in a Latin square design with four 21-d experimental periods to determine effects of feeding extruded versus non-extruded canola seed, with or without 50 g/kg lignosulfonate on rumen fermentation, nutrient flow to the omasum, and degradability of dry matter (DM) and N of each diet. The DM effective degradability increased with extrusion and lignosulfonate treatment had no effect. The effective degradability of N was similar between diets. Lignosulfonate treatment of extruded versus non-extruded canola seeds decreased ruminal and total tract apparent digestibility of organic matter. The lowest apparent ruminal and highest intestinal digestibilities of protein, expressed as a percentage of N intake were observed for cows fed extruded canola seeds without lignosulfonate. Lignosulfonate treatment and extrusion had no effect on pH and concentrations of ammonia N and volatile fatty acids in the rumen. Results suggest that extruded canola seed untreated with formaldehyde may stimulate efficiency of microbial protein synthesis and is an effective means of increasing the availability of protein in the small intestine without affecting the total tract apparent digestibility of protein. © 2012 Sociedade Brasileira de Zootecnia. Source


Amorim W.P.,Federal University of Mato Grosso | Pistori H.,UCDB | Pereira M.C.,UCDB | Jacinto M.A.C.,EMBRAPA - Empresa Brasileira de Pesquisa Agropecuaria
Proceedings - 23rd SIBGRAPI Conference on Graphics, Patterns and Images, SIBGRAPI 2010 | Year: 2010

This paper presents a study on attributes reduction, comparing five discriminant analysis techniques: Fisher-Face, CLDA, DLDA, YLDA and KLDA. Attributes reduction has been applied to the problem of leather defect classification using four different classifiers: C4.5, kNN, Naïve Bayes and Support Vector Machines. The results of several experiments on the performance of discriminant analysis applied to the problem of defect detection are reported © 2010 IEEE. Source


Nogueira E.,EMBRAPA - Empresa Brasileira de Pesquisa Agropecuaria | Cardoso G.S.,UCDB | Junior H.R.M.,UCDB | Dias A.M.,UCDB | And 2 more authors.
Revista Brasileira de Zootecnia | Year: 2012

The objective of this study was to evaluate pregnancy rates of recipients of different breed groups (Nellore and crossbreed), as well as the effects of size and type of the corpus luteum (CL) on plasmatic concentrations of progesterone and pregnancy rates of embryo recipients. A total of 152 heifers were synchronized with progesterone implants and on the day of embryo transfer, previously obtained by superovulation and frozen in ethylene glycol, the diameter and type of the corpus luteum (cavitary and compact) was measured and blood was collected for progesterone measurement. The pregnancy rate was 44.1%, with a diameter of corpus luteum higher in recipients that became pregnant (2.03±0.41) compared with nonpregnant ones (1.86±0.34 cm). Plasmatic concentrations of progesterone did not differ between pregnant (1.50±1.05) and non-pregnant (1.31±0.91 ng/mL) animals. The type of corpus luteum did not influence the pregnancy rates. Only Angus and crossbred Marchigiana differ among themselves in pregnancy rates (33.3 and 59.2%, respectively). The pregnancy probability was affected only by CL diameter, but not by P4 plasmatic concentration. Selection of the corpus luteum size at the time of embryo transfer is an important factor to increase pregnancy rates in recipients, and compact and cavitary corpora lutea do not influence the pregnancy rates of bovine embryo recipients. Nellore recipients have pregnancy rates that are satisfactory and comparable to crossbred (Bos taurus × Bos indicus) recipients. ©2012 Sociedade Brasileira de Zootecnia. Source


Nogueira E.,EMBRAPA - Empresa Brasileira de Pesquisa Agropecuaria | Da Silva A.S.,Genesis Reproducao Animal | Amaral T.B.,EMBRAPA - Empresa Brasileira de Pesquisa Agropecuaria | Itavo L.C.V.,UCDB | And 2 more authors.
Revista Brasileira de Zootecnia | Year: 2012

To verify the effects of energy supplementation and fat on follicular dynamics, metabolic profile and the production of oocytes, 15 young heifers (Bos taurus indicus) with an average age of 14 months and with an initial weight of 256.35 kg were assigned to two treatments according to weight and follicular population as evaluated by ultrasonography: in T1 (1.0 × M), animals received 100% of the energy requirements for maintenance; in T2 (1.7 × M), animals received 170% of the energy requirements for maintenance, achieved by the addition of 200 g of Megalac®. After a period of adaptation to the diet, the treatments, blood collection and follicular aspirations were started using a randomized design. The dry matter intake and weight gain were lower in the 1.0 × M group than in the 1.7 × M group. No differences were found in the plasma progesterone concentrations, albumin, glucose, urea or gonadotropin (FSH and LH) levels between the groups. The mean concentrations of cholesterol were higher in the 1.7 × M group. The total number of small (<4 mm) and medium follicles (4-8 mm) was not altered by the treatments, but the number of small follicles increased on days 1 and 2 of the estrous cycle, with higher values found in the 1.7 × M group. The average of the oocytes also increased (9.50±2.1 and 12.5±4.4 for the 1.0 × M and 1.7 × M groups, respectively). The rapid increase in the amount of energy offered in the diet changes the amount of follicles and oocytes available for follicular aspiration (OPU) in young heifers without changing their metabolic profile. © 2012 Sociedade Brasileira de Zootecnia. Source

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