Pinyachat A.,Ubon Ratchathani UniversityUbon Ratchathani
Journal of the Medical Association of Thailand | Year: 2016
Snake venom metalloproteinases (SVMPs) induces local and systemic effects on patients suffering from snakebite, degrading extracellular matrix (ECM) proteins such as collagen, gelatin, elastin, laminin, fibronectin, nidogen (entactin), and thrombospondin that cause local hemorrhage and tissue damage. They cleave or activate coagulation factors such as fibrinogen, fibrin, prothrombin, factor V, factor IX, factor X and protein C that bring about systemic coagulopathy. SVMPs and their truncated forms cleave or interfere with platelet adhesive proteins such as vWF, fibrinogen and collagen, and cleave or interfere with platelet receptors such as GPVI, alpha2beta1, GPIb, GPIX, and GPIIbIIIa that result in platelet aggregation defect. SVMPs induce cancer cell line to form morphological changes and apoptosis in vitro concordant with skin necrosis after snakebite in some cases. These local effects caused by SVMPs have no certain treatments, even with commercial antivenom. SVMPs researches are focusing on their inhibitors, measurement and replacement of blood coagulation factor defects, or anti-cancer drug. © 2016, Medical Association of Thailand. All rights reserved.
Yuajit C.,Ubon Ratchathani UniversityUbon Ratchathani |
Chatsudthipong V.,Mahidol University
Journal of the Medical Association of Thailand | Year: 2016
Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited renal disorder caused by mutations of either PKD1 or PKD2 gene. Cyst formation initiates from a combination of abnormal cell proliferation along with enhanced fluid secretion. ADPKD is characterized by the progressive enlargement of cysts which destroy the renal parenchymal cells, resulting in renal failure. Currently, there is no effective treatment for this disease. Interestingly, several relevant therapeutic effects of herbal medicine relevant to pathogenic process of ADPKD have urged the researchers to search for potential candidate herb as nutraceutical for ADPKD therapy. Up to now, several natural compounds, such as triptolide, curcumin, ginkolide B, and steviol (stevia extract) have been shown to be able to retard cyst progression in ADPKD. The detailed mechanism of these compounds showed that triptolide enhanced calcium restoration, curcumin inhibited ERK & p-STAT 3 pathways, ginkolide B inhibited Ras/MAPK pathway, and steviol activated AMPK, which inhibited CFTR channel and mTOR pathway in cell and mouse models of PKD. In addition, they are currently in preclinical and clinical studies, respectively. This review focuses on the pathophysiology of ADPKD and the recent therapeutic approaches, especially a potential use of nutraceutical for the treatment of autosomal dominant polycystic kidney disease. © 2016, Medical Association of Thailand. All rights reserved.
Pilajun R.,Ubon Ratchathani UniversityUbon Ratchathani |
Wanapat M.,Khon Kaen University
Pakistan Journal of Nutrition | Year: 2014
An in vitro study was conducted to evaluate effect of roughage to concentrate ratio (R:C) and coconut oil (CO) and wild almond seed oil (WO) supplementation on gas production, volatile fatty acid (VFA) concentration, methane production and dry matter disappearance. Completely randomized design was used for sixteen treatments. Treatments were 4x4 factorial arrangement where four of R:C ratios (100:0, 75:25, 50:50 and 25:75) and four of oil supplementation (un-supplement, 5% CO, 5% WO and 2.5% CO+2.5% WO). The potential extent of gas production was quadratically responded to R:C ratio (p<0.01) while gas production from all fractions of feed were suppressed by oil supplementation (p<0.05). Total VFA production, propionic acid proportion and calculated methane production were linearly increased with concentrate ratio; while oil supplementation decreased (p<0.05) these parameters especially when supplemented with wild almond seed oil. Dry matter disappearance at 24 h of incubation was increased with concentrate quantity but decreased by oil supplementation (p<0.05). It could be concluded that R:C ratio and seed oil remarkably influenced on fermentation end-product and gas production. © Asian Network for Scientific Information, 2014.
Yingngam B.,University of Graz |
Yingngam B.,Ubon Ratchathani UniversityUbon Ratchathani |
Monschein M.,University of Graz |
Brantner A.H.,University of Graz
Chiang Mai Journal of Science | Year: 2016
The ripe fruits of Antidesma puncticulatum are used as commercial sources for phenolic compounds and anthocyanins for functional foods. The aim of this study was to optimize the process parameters for extraction of phenolic and anthocyanin compounds with good antioxidant activity from the fruits using ultrasonic assisted extraction (UAE). A four-factor, five-level, six-center point central composite design was performed. Effects of solvent-tosolid ratio (X1: 7.5-37.5 mL/g), acidified ethanol solution (X2: 0-80%), extraction temperature (X3: 20-100°C), and extraction time (X4: 0-20 min) on the recovery of total phenolic and total anthocyanin contents with good antioxidant activity were investigated. The second order polynomial models of the response variables were obtained with an R2 of 0.9455, 0.9308 and 0.8998, respectively. The optimal extraction conditions to obtain maximum yields of targeted compounds were 30 mL/g X1, 45% (v/v) X2, 80°C X3 and 12 min X2. The experimental values were in good agreement with the predicted values under the optimized conditions. The major constituents in the optimized extract were organic acids whereas phenolic compounds were minor components. Delphinidin-3-sambubioside-5-rhamnoside and delphinidin were identified by HPLC-ESI/MS as major anthocyanins in the extract. The plant extract showed moderate inhibitory activity on mushroom tyrosinase with a non-competitive inhibitory mechanism. The UAE can be considered as an effective method for extracting the biologically active compounds from fruits of A. puncticulatum. © 2016, Chiang Mai University. All rights reserved.
Chaiwong T.,Ubon Ratchathani UniversityUbon Ratchathani |
Tem-Eiam N.,Sisaket HospitalSisaket |
Limpavithayakul M.,Office of Disease Prevention and Control 7Ubon Ratchathani |
Boongunha N.,Sisaket HospitalSisaket |
And 2 more authors.
Tropical Biomedicine | Year: 2014
Herein is reported the first case in Thailand of aural myiasis caused by the flesh fly, Parasarcophaga (Liosarcophaga) dux (Thomson). A 5-day-old infant was taken to hospital with a slightly bloody ear. Two fly larvae exiting the ear and another recovered by a physician were alive, and confirmed as P. dux species from adult examination results. This case brought attention to the need for protection against synanthropic flies, particularly for infants and/or hearing impaired patients. © 2014 MSPTM All rights reserved.