Ubon Ratchathani UniversityUbon Ratchathani

Ubon Ratchathani, Thailand

Ubon Ratchathani UniversityUbon Ratchathani

Ubon Ratchathani, Thailand
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Duangjit S.,Ubon Ratchathani UniversityUbon Ratchathani | Kraisit P.,Thammasat UniversityPathumthani | Luangtana-Anan M.,Silpakorn UniversityNakhon Pathom
Thai Journal of Pharmaceutical Sciences | Year: 2016

Objectives: The objectives of this study were to develop the propranolol-loaded chitosan nanoparticles for transmucosal delivery and investigate the effect of molecular weight of chitosan and concentrations of propranolol on physical properties, i.e. pH, zeta potential and particle size of nanoparticles using the Design Expert®. Methods: The investigated nanoparticles composed of tripolyphosphate (0.1 %w/v), various molecular weights of chitosan solution (0.2% w/v) (at 20, 35, 45 kDa), and various concentrations of propranolol at 10, 20 and 30 mg/mL were prepared and characterized for pH, zeta potential and particle size. The data obtained were analyzed using conventional comparative and response surface method. Results: The results indicated that the response surface method showed the relationship between the formulation factors and the physical properties of nanoparticles was more markedly than the conventional comparative method. Using the response surface, the physical properties of other nanoparticles over the model formulations can be predicted. Whiles the conventional comparative method can be explained only the physical properties of model formulations. The formulation factors with a significant influence on pH, zeta potential and particle size were the molecular weight of chitosan (X1) and concentrations of propranolol (X2). The X1 supported a positive influence on pH and particle size, whiles X2 supported a positive influence on particle size. The linear correlation coefficient results for pH and zeta potential were relatively high (0.7–0.9) indicating the reliability of the response surface. Moreover, the vertical spread of the internally studentized residuals was not out of the line from bottom-to-top, indicating all points fall within the limits (at 95% confidence level) Conclusion: The formulation factors i.e. the molecular weight of chitosan and the concentrations of propranolol significantly affected the physical properties, i.e. pH, zeta potential and particle size of the nanoparticles. Using the response surface method, the relationship between the formulation factors and the physical properties was clearly understood. In this study, we were successful in showing the feasibility of the development of transmucosal delivery of propranolol using nanoparticles. Further study is required to confirm the potential of nanoparticels by in vitro and in vivo drug penetration study. © 2016, Faculty of Pharmaceutical Sciences, Chulalongkorn University. All rights reserved.

Chaiwong T.,Ubon Ratchathani UniversityUbon Ratchathani | Tem-Eiam N.,Sisaket HospitalSisaket | Limpavithayakul M.,Office of Disease Prevention and Control 7Ubon Ratchathani | Boongunha N.,Sisaket HospitalSisaket | And 2 more authors.
Tropical Biomedicine | Year: 2014

Herein is reported the first case in Thailand of aural myiasis caused by the flesh fly, Parasarcophaga (Liosarcophaga) dux (Thomson). A 5-day-old infant was taken to hospital with a slightly bloody ear. Two fly larvae exiting the ear and another recovered by a physician were alive, and confirmed as P. dux species from adult examination results. This case brought attention to the need for protection against synanthropic flies, particularly for infants and/or hearing impaired patients. © 2014 MSPTM All rights reserved.

Yoonaiwong W.,Ubon Ratchathani UniversityUbon Ratchathani | Kaewsarn P.,Ubon Ratchathani UniversityUbon Ratchathani | Reanprayoon P.,Surindra Rajabhat University
Sustainable Environment Research | Year: 2011

Biosorption of Pb(II) and Cd(II) ions by non-living macrophytes, Utricularia aurea has been investigated as a function of initial pH, contact time and metal concentrations. Adsorption kinetic and equilibrium tests were carried out in flasks under batch operations. The adsorption data obtained under optimum condition were evaluated by Freundlich and Langmuir isotherm. The biosorption kinetic results have shown that Pb(II) and Cd(II) ions removal rate was at its maximum at the beginning of the process. The equilibrium state was achieved after 30 min for lead and 90 min for cadmium. When experiments were performed with different desorbents the results indicated that 0.1 M HCl was an efficient desorbent for recovery of metal ions from biomass. In addition, Fourier transform infrared spectroscopy (FTIR) spectroscopy was done to identify the chemical functional group present on the biomass. The surface structure of biomass was analyzed by scanning electron microscopy (SEM). The biosorption mechanism of biosorbent was also evaluated by FTIR and SEM. © 2011, Chinese Institute of Environmental Engineering. All rights reserved.

Yingngam B.,University of Graz | Yingngam B.,Ubon Ratchathani UniversityUbon Ratchathani | Monschein M.,University of Graz | Brantner A.H.,University of Graz
Chiang Mai Journal of Science | Year: 2016

The ripe fruits of Antidesma puncticulatum are used as commercial sources for phenolic compounds and anthocyanins for functional foods. The aim of this study was to optimize the process parameters for extraction of phenolic and anthocyanin compounds with good antioxidant activity from the fruits using ultrasonic assisted extraction (UAE). A four-factor, five-level, six-center point central composite design was performed. Effects of solvent-tosolid ratio (X1: 7.5-37.5 mL/g), acidified ethanol solution (X2: 0-80%), extraction temperature (X3: 20-100°C), and extraction time (X4: 0-20 min) on the recovery of total phenolic and total anthocyanin contents with good antioxidant activity were investigated. The second order polynomial models of the response variables were obtained with an R2 of 0.9455, 0.9308 and 0.8998, respectively. The optimal extraction conditions to obtain maximum yields of targeted compounds were 30 mL/g X1, 45% (v/v) X2, 80°C X3 and 12 min X2. The experimental values were in good agreement with the predicted values under the optimized conditions. The major constituents in the optimized extract were organic acids whereas phenolic compounds were minor components. Delphinidin-3-sambubioside-5-rhamnoside and delphinidin were identified by HPLC-ESI/MS as major anthocyanins in the extract. The plant extract showed moderate inhibitory activity on mushroom tyrosinase with a non-competitive inhibitory mechanism. The UAE can be considered as an effective method for extracting the biologically active compounds from fruits of A. puncticulatum. © 2016, Chiang Mai University. All rights reserved.

Prakrankamanant P.,Ubon Ratchathani UniversityUbon Ratchathani | Wongsena M.,Ubon Ratchathani Cancer HospitalUbon Ratchathani
Journal of the Medical Association of Thailand | Year: 2016

Human papillomavirus (HPV) is the most common sexually-transmitted virus and it is known that persistent infection by high-risk HPV is a necessary factor for cervical carcinogenesis. Although cytological screening has decreased the incidence of cervical cancer, the sensitivity and specificity of testing is limited. To date, HPV-driven molecular techniques have provided a number of potential biomarkers for both diagnostic and prognostic use in clinical management. In addition, they can provide insights into the biology of HPV-induced cancers leading to non-surgical therapy. This review summarizes current knowledge of detection methods for HPV and related biomarkers that can be used to discriminate lesions with a high risk of progression of cervical cancer. © 2016, Medical Association of Thailand. All rights reserved.

Pinyachat A.,Ubon Ratchathani UniversityUbon Ratchathani
Journal of the Medical Association of Thailand | Year: 2016

Snake venom metalloproteinases (SVMPs) induces local and systemic effects on patients suffering from snakebite, degrading extracellular matrix (ECM) proteins such as collagen, gelatin, elastin, laminin, fibronectin, nidogen (entactin), and thrombospondin that cause local hemorrhage and tissue damage. They cleave or activate coagulation factors such as fibrinogen, fibrin, prothrombin, factor V, factor IX, factor X and protein C that bring about systemic coagulopathy. SVMPs and their truncated forms cleave or interfere with platelet adhesive proteins such as vWF, fibrinogen and collagen, and cleave or interfere with platelet receptors such as GPVI, alpha2beta1, GPIb, GPIX, and GPIIbIIIa that result in platelet aggregation defect. SVMPs induce cancer cell line to form morphological changes and apoptosis in vitro concordant with skin necrosis after snakebite in some cases. These local effects caused by SVMPs have no certain treatments, even with commercial antivenom. SVMPs researches are focusing on their inhibitors, measurement and replacement of blood coagulation factor defects, or anti-cancer drug. © 2016, Medical Association of Thailand. All rights reserved.

Yuajit C.,Ubon Ratchathani UniversityUbon Ratchathani | Chatsudthipong V.,Mahidol University
Journal of the Medical Association of Thailand | Year: 2016

Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited renal disorder caused by mutations of either PKD1 or PKD2 gene. Cyst formation initiates from a combination of abnormal cell proliferation along with enhanced fluid secretion. ADPKD is characterized by the progressive enlargement of cysts which destroy the renal parenchymal cells, resulting in renal failure. Currently, there is no effective treatment for this disease. Interestingly, several relevant therapeutic effects of herbal medicine relevant to pathogenic process of ADPKD have urged the researchers to search for potential candidate herb as nutraceutical for ADPKD therapy. Up to now, several natural compounds, such as triptolide, curcumin, ginkolide B, and steviol (stevia extract) have been shown to be able to retard cyst progression in ADPKD. The detailed mechanism of these compounds showed that triptolide enhanced calcium restoration, curcumin inhibited ERK & p-STAT 3 pathways, ginkolide B inhibited Ras/MAPK pathway, and steviol activated AMPK, which inhibited CFTR channel and mTOR pathway in cell and mouse models of PKD. In addition, they are currently in preclinical and clinical studies, respectively. This review focuses on the pathophysiology of ADPKD and the recent therapeutic approaches, especially a potential use of nutraceutical for the treatment of autosomal dominant polycystic kidney disease. © 2016, Medical Association of Thailand. All rights reserved.

Wongbutdee J.,Ubon Ratchathani UniversityUbon Ratchathani | Jittimanee J.,Ubon Ratchathani UniversityUbon Ratchathani
Journal of the Medical Association of Thailand | Year: 2016

Background: Leptospirosis, a zoonotic disease caused by Leptospira, has been a health problem in Thailand for several years. Rats are a major reservoir host for Leptospira, and the people who are usually in contact with environments contaminated with rats’ urine are at risk of infection. The prevalence rate of Leptospira infection in rats may result in the spread of leptospirosis in humans. Objective: This study aimed to determine the prevalence rate of Leptospira infection in a total of 28 rats and develop a spatial database for leptospirosis surveillance in Phraroj village in Muang Sam Sip District, Ubon Ratchathani Province. Material and Method: The positions of the households and the rat-trapping area were tagged by using of a Global Positioning System (GPS). DNA samples were isolated from rats’ kidneys. The polymerase chain reaction (PCR) technique was used for the detection of 16s rRNA and LipL32 genes specific to genus and pathogenic Leptospira respectively. All of the data were used to develop a geo-data base by the connection of spatial data and attributed data to be used for query and retrieval. Results: A map of the positions of the households and the rat-trapping area in Phraroj village was created. No rats were found to be infected in the Leptospira survey. Conclusion: There was no trapped rat infected with Leptospira in Phraroj village. This result may involve unreported leptospirosis in patients in this village. The Leptospira survey in rats and the geo-database will be used as a primary resource to support and make decisions about surveillance, prevention, and control of leptospirosis. © 2016, Medical Association of Thailand. All rights reserved.

Pilajun R.,Ubon Ratchathani UniversityUbon Ratchathani | Wanapat M.,Khon Kaen University
Pakistan Journal of Nutrition | Year: 2014

An in vitro study was conducted to evaluate effect of roughage to concentrate ratio (R:C) and coconut oil (CO) and wild almond seed oil (WO) supplementation on gas production, volatile fatty acid (VFA) concentration, methane production and dry matter disappearance. Completely randomized design was used for sixteen treatments. Treatments were 4x4 factorial arrangement where four of R:C ratios (100:0, 75:25, 50:50 and 25:75) and four of oil supplementation (un-supplement, 5% CO, 5% WO and 2.5% CO+2.5% WO). The potential extent of gas production was quadratically responded to R:C ratio (p<0.01) while gas production from all fractions of feed were suppressed by oil supplementation (p<0.05). Total VFA production, propionic acid proportion and calculated methane production were linearly increased with concentrate ratio; while oil supplementation decreased (p<0.05) these parameters especially when supplemented with wild almond seed oil. Dry matter disappearance at 24 h of incubation was increased with concentrate quantity but decreased by oil supplementation (p<0.05). It could be concluded that R:C ratio and seed oil remarkably influenced on fermentation end-product and gas production. © Asian Network for Scientific Information, 2014.

Sroysee W.,Ubon Ratchathani UniversityUbon Ratchathani | Ponlakhet K.,Ubon Ratchathani UniversityUbon Ratchathani | Chairam S.,Ubon Ratchathani UniversityUbon Ratchathani | Jarujamrus P.,Ubon Ratchathani UniversityUbon Ratchathani | Amatatongchai M.,Ubon Ratchathani UniversityUbon Ratchathani
Talanta | Year: 2016

We describe a novel amperometric sulfite biosensor, comprising a carbon-paste electrode (Fe3O4@Au-Cys-FA/CPE) modified with immobilized sulfite oxidase (SOx) on a gold-coated magnetite nanoparticle core, encased within a conjugated folic acid (FA) cysteine (Cys) shell. The biosensor electrode was fabricated using a polydimethylsiloxane (PDMS) and mineral oil mixture as binder, which also enhances the physical stability and sensitivity of the electrode. The developed biosensor displays good electrocatalytic activity toward oxidation of H2O2, which occurs by an enzymatic reaction between SOx and sulfite. The Fe3O4@Au-Cys-FA electrode exhibits good electrocatalytic activity, and has good retention of chemisorbed SOx on the electrode because of its large surface area. Sulfite was quantified using amperometric measurements from the Fe3O4@Au-Cys-FA/CPE biosensor, and using an in-house assembled flow cell at +0.35 V (vs. Ag/AgCl), with a phosphate buffer carrier (0.10 M, pH 7.0) at a flow rate of 0.8 mL min−1. The system detects sulfite over the range 0.1–200 mg L−1 (r2=0.998), with a detection limit of 10 µg L−1 (3σ of blank). The system exhibits acceptable precision (%R.S.D.=3.1%), rapid sample throughput (109 samples h−1), and good stability (2 w). The developed biosensor shows satisfactory tolerance to potential interferences, such as sugars, anions, ascorbic acid, and ethanol. We applied the developed method to the determination of sulfite content in wines and pickled food extracts, and our results are in good agreement with those obtained by the standard iodometric method. © 2016 Elsevier B.V.

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